1887

Abstract

A novel Gram-positive, non-motile, rod-shaped bacterium, designated strain RB-62, was isolated during a study of culturable bacteria from the gut of (Pascoe) and its taxonomic position was investigated. Strain RB-62 grew at 15–30 °C and pH 5.0–8.5. The isoprenoid quinones were menaquinones MK-11 (77.1 %), MK-10 (11.7 %) and MK-12 (11.2 %). The major cellular fatty acids were anteiso-C (34.6 %), anteiso-C (29.8 %), iso-C (17.0 %) and cyclohexyl-C (11.4 %). The diagnostic diamino acid of the cell-wall peptidoglycan was 2,4-diaminobutyric acid. The G+C content of the genomic DNA of strain RB-62 was 70.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RB-62 was affiliated with the genus cluster within the family , and was related most closely to wged11 (98.08 % similarity). The level of DNA–DNA relatedness between strain RB-62 and wged11 was 43.2 % (reciprocal 66.7 %). Phenotypic and phylogenetic characteristics clearly distinguished strain RB-62 from recognized species of the genus . Based on data from the present polyphasic study, strain RB-62 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RB-62 ( = KCTC 19653 = JCM 16117).

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2012-01-01
2020-01-25
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