RT Journal Article SR Electronic(1) A1 Henson, Brian J. A1 Hartman, Linda A1 Watson, Linda E. A1 Barnum, Susan R.YR 2011 T1 Evolution and variation of the nifD and hupL elements in the heterocystous cyanobacteria JF International Journal of Systematic and Evolutionary Microbiology, VO 61 IS 12 SP 2938 OP 2949 DO https://doi.org/10.1099/ijs.0.028340-0 PB Microbiology Society, SN 1466-5034, AB In heterocystous cyanobacteria, heterocyst differentiation is accompanied by developmentally regulated DNA rearrangements that occur within the nifD and hupL genes, referred to as the nifD and hupL elements. These elements are segments of DNA that are embedded within the coding region of each gene and range from 4 to 24 kb in length. The nifD and hupL elements are independently excised from the genome during the later stages of differentiation by the site-specific recombinases, XisA and XisC, respectively, which are encoded within the elements themselves. Here we examine the variation and evolution of the nifD and hupL elements by comparing full-length nifD and hupL element sequences and by phylogenetic analysis of xisA and xisC gene sequences. There is considerable variation in the size and composition of the nifD and hupL elements, however, conserved regions are also present within representatives of each element. The data suggest that the nifD and hupL elements have undergone a complex pattern of insertions, deletions, translocations and sequence divergence over the course of evolution, but that conserved regions remain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.028340-0