sp. nov., an actinobacterium of the family isolated from lichen, and emended description of the genus Free

Abstract

A novel actinobacterium, designated strain CafT13, was isolated from the thallus of the reindeer lichen sampled in the Austrian Alps (Koralpe). The organism was aerobic, with rod- to irregular-shaped cells (often forming dense clusters of cells when grown in liquid medium), Gram-stain-positive, oxidase-negative, catalase-positive and non-motile. It was able to grow at 1 °C and at low to neutral pH, but not above 30 °C or at high pH. The peptidoglycan type was B2β with ornithine as the diagnostic diamino acid. The menaquinones were MK-7 and MK-8. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, three unidentified glycolipids and one unidentified aminolipid. The predominant fatty acids were C, C 2-OH, Cω9, C and anteiso-C. The mean DNA G+C content of strain CafT13 was 69.0±0.17 mol%. 16S rRNA gene sequence analysis showed that strain CafT13 belongs to the family , within the genus . The mean level of DNA–DNA relatedness between strain CafT13 and the type strain of was 35.2±5.23 %. The enzyme spectrum of strain CafT13 differentiated it from recognized species of the genus . Based on molecular, chemotaxonomic and physiological data, strain CafT13 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is CafT13 ( = DSM 23273 = LMG 25550).

Funding
This study was supported by the:
  • Austrian Science Foundation (Award E-1653P19098)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.028324-0
2011-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/12/3033.html?itemId=/content/journal/ijsem/10.1099/ijs.0.028324-0&mimeType=html&fmt=ahah

References

  1. Bates S. T., Cropsey G. W. G., Caporaso J. G., Knight R., Fierer N. 2011; Bacterial communities associated with the lichen symbiosis. Appl Environ Microbiol 77:1309–1314 [View Article][PubMed]
    [Google Scholar]
  2. Bjelland T., Grube M., Hoem S., Jorgensen S. L., Daae F. L., Thorseth I. H., Øvreås L. 2011; Microbial metacommunities in the lichen–rock habitat. Environ Microbiol Rep 3:434–442 [View Article]
    [Google Scholar]
  3. Bright M., Bulgheresi S. 2010; A complex journey: transmission of microbial symbionts. Nat Rev Microbiol 8:218–230 [View Article][PubMed]
    [Google Scholar]
  4. Cardinale M., Vieira de Castro J. Jr, Müller H., Berg G., Grube M. 2008; In situ analysis of the bacterial community associated with the reindeer lichen Cladonia arbuscula reveals predominance of Alphaproteobacteria . FEMS Microbiol Ecol 66:63–71 [View Article][PubMed]
    [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  7. Eck R. V., Dayhoff M. O. 1966 Atlas of Protein Sequence and Structure Silver Springs, MD: National Biomedical Research Foundation;
    [Google Scholar]
  8. Felsenstein J. 1989; phylip – phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  9. Felsenstein J. 2005; phylip (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle
  10. Greene A. C., Euzéby J. P., Tindall B. J., Patel B. K. C. 2009; Proposal of Frondihabitans gen. nov. to replace the illegitimate genus name Frondicola Zhang et al. 2007. Int J Syst Evol Microbiol 59:447–448 [View Article][PubMed]
    [Google Scholar]
  11. Grube M., Berg G. 2009; Microbial consortia of bacteria and fungi with focus on the lichen symbiosis. Fungal Biol Rev 23:72–85 [View Article]
    [Google Scholar]
  12. Grube M., Cardinale M., de Castro J. V. Jr, Müller H., Berg G. 2009; Species-specific structural and functional diversity of bacterial communities in lichen symbioses. ISME J 3:1105–1115 [View Article][PubMed]
    [Google Scholar]
  13. Hodkinson B. P., Lutzoni F. 2009; A microbiotic survey of lichen-associated bacteria reveals a new lineage from the Rhizobiales. Symbiosis 49:163–180 [View Article]
    [Google Scholar]
  14. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  15. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp. 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  16. Kidd K. K., Sgaramella-Zonta L. A. 1971; Phylogenetic analysis: concepts and methods. Am J Hum Genet 23:235–252[PubMed]
    [Google Scholar]
  17. Kluge, Farris J. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [View Article]
    [Google Scholar]
  18. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. et al. other authors 2007; Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  19. Lee S. D. 2010; Frondihabitans peucedani sp. nov., an actinobacterium isolated from rhizosphere soil, and emended description of the genus Frondihabitans Greene et al. 2009. Int J Syst Evol Microbiol 60:1740–1744 [View Article][PubMed]
    [Google Scholar]
  20. MacKenzie S. L. 1984; Amino acids and peptides. In Gas Chromatography/Mass Spectrometry Applications . in Microbiology pp. 157–204 Edited by Odham G., Larsson L., Mardh P. New York: Plenum;
    [Google Scholar]
  21. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  22. Miller J. A., Kalyuzhnaya M. G., Noyes E., Lara J. C., Lidstrom M. E., Chistoserdova L. 2005; Labrys methylaminiphilus sp. nov., a novel facultatively methylotrophic bacterium from a freshwater lake sediment. Int J Syst Evol Microbiol 55:1247–1253 [View Article][PubMed]
    [Google Scholar]
  23. Rzhetsky A., Nei M. 1993; Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10:1073–1095[PubMed]
    [Google Scholar]
  24. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  25. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  26. Schneider T., Schmid E., de Castro J. V. Jr, Cardinale M., Eberl L., Grube M., Berg G., Riedel K. 2011; Structure and function of the symbiosis partners of the lung lichen (Lobaria pulmonaria L. Hoffm.) analyzed by metaproteomics. Proteomics 11:2752–2756 [View Article][PubMed]
    [Google Scholar]
  27. Schumann P., Kämpfer P., Busse H. J., Evtushenko L. I. Subcommittee on the Taxonomy of the Suborder Micrococcineae of the International Committee on Systematics of Prokaryotes 2009; Proposed minimal standards for describing new genera and species of the suborder Micrococcineae . Int J Syst Evol Microbiol 59:1823–1849 [View Article][PubMed]
    [Google Scholar]
  28. Selbmann L., Zucconi L., Ruisi S., Grube M., Cardinale M., Onofri S. 2010; Culturable bacteria associated with Antarctic lichens: affiliation and psychrotolerance. Polar Biol 33:71–83 [View Article]
    [Google Scholar]
  29. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  30. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  31. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  32. Tindall B. J., Kämpfer P., Euzéby J. P., Oren A. 2006; Valid publication of names of prokaryotes according to the rules of nomenclature: past history and current practice. Int J Syst Evol Microbiol 56:2715–2720 [View Article][PubMed]
    [Google Scholar]
  33. Tittsler R. P., Sandholzer L. A. 1936; The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31:575–580[PubMed]
    [Google Scholar]
  34. Zhang L., Xu Z., Patel B. K. C. 2007; Frondicola australicus gen. nov., sp. nov., isolated from decaying leaf litter from a pine forest. Int J Syst Evol Microbiol 57:1177–1182 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.028324-0
Loading
/content/journal/ijsem/10.1099/ijs.0.028324-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed