1887

Abstract

A novel actinobacterium, designated strain CafT13, was isolated from the thallus of the reindeer lichen sampled in the Austrian Alps (Koralpe). The organism was aerobic, with rod- to irregular-shaped cells (often forming dense clusters of cells when grown in liquid medium), Gram-stain-positive, oxidase-negative, catalase-positive and non-motile. It was able to grow at 1 °C and at low to neutral pH, but not above 30 °C or at high pH. The peptidoglycan type was B2β with ornithine as the diagnostic diamino acid. The menaquinones were MK-7 and MK-8. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, three unidentified glycolipids and one unidentified aminolipid. The predominant fatty acids were C, C 2-OH, Cω9, C and anteiso-C. The mean DNA G+C content of strain CafT13 was 69.0±0.17 mol%. 16S rRNA gene sequence analysis showed that strain CafT13 belongs to the family , within the genus . The mean level of DNA–DNA relatedness between strain CafT13 and the type strain of was 35.2±5.23 %. The enzyme spectrum of strain CafT13 differentiated it from recognized species of the genus . Based on molecular, chemotaxonomic and physiological data, strain CafT13 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is CafT13 ( = DSM 23273 = LMG 25550).

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2011-12-01
2019-10-14
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References

  1. Bates S. T. , Cropsey G. W. G. , Caporaso J. G. , Knight R. , Fierer N. . ( 2011; ). Bacterial communities associated with the lichen symbiosis. . Appl Environ Microbiol 77:, 1309–1314. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bjelland T. , Grube M. , Hoem S. , Jorgensen S. L. , Daae F. L. , Thorseth I. H. , Øvreås L. . ( 2011; ). Microbial metacommunities in the lichen–rock habitat. . Environ Microbiol Rep 3:, 434–442. [CrossRef]
    [Google Scholar]
  3. Bright M. , Bulgheresi S. . ( 2010; ). A complex journey: transmission of microbial symbionts. . Nat Rev Microbiol 8:, 218–230. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cardinale M. , Vieira de Castro J. Jr , Müller H. , Berg G. , Grube M. . ( 2008; ). In situ analysis of the bacterial community associated with the reindeer lichen Cladonia arbuscula reveals predominance of Alphaproteobacteria . . FEMS Microbiol Ecol 66:, 63–71. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cashion P. , Holder-Franklin M. A. , McCully J. , Franklin M. . ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef] [PubMed]
    [Google Scholar]
  6. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  7. Eck R. V. , Dayhoff M. O. . ( 1966; ). Atlas of Protein Sequence and Structure. Silver Springs, MD:: National Biomedical Research Foundation;.
    [Google Scholar]
  8. Felsenstein J. . ( 1989; ). phylip – phylogeny inference package (version 3.2). . Cladistics 5:, 164–166.
    [Google Scholar]
  9. Felsenstein J. . ( 2005; ). phylip (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle.
  10. Greene A. C. , Euzéby J. P. , Tindall B. J. , Patel B. K. C. . ( 2009; ). Proposal of Frondihabitans gen. nov. to replace the illegitimate genus name Frondicola Zhang et al. 2007. . Int J Syst Evol Microbiol 59:, 447–448. [CrossRef] [PubMed]
    [Google Scholar]
  11. Grube M. , Berg G. . ( 2009; ). Microbial consortia of bacteria and fungi with focus on the lichen symbiosis. . Fungal Biol Rev 23:, 72–85. [CrossRef]
    [Google Scholar]
  12. Grube M. , Cardinale M. , de Castro J. V. Jr , Müller H. , Berg G. . ( 2009; ). Species-specific structural and functional diversity of bacterial communities in lichen symbioses. . ISME J 3:, 1105–1115. [CrossRef] [PubMed]
    [Google Scholar]
  13. Hodkinson B. P. , Lutzoni F. . ( 2009; ). A microbiotic survey of lichen-associated bacteria reveals a new lineage from the Rhizobiales. . Symbiosis 49:, 163–180. [CrossRef]
    [Google Scholar]
  14. Huß V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192.[CrossRef]
    [Google Scholar]
  15. Jukes T. H. , Cantor C. R. . ( 1969; ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N. . . New York:: Academic Press;.
    [Google Scholar]
  16. Kidd K. K. , Sgaramella-Zonta L. A. . ( 1971; ). Phylogenetic analysis: concepts and methods. . Am J Hum Genet 23:, 235–252.[PubMed]
    [Google Scholar]
  17. Kluge , Farris J. S. . ( 1969; ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32. [CrossRef]
    [Google Scholar]
  18. Larkin M. A. , Blackshields G. , Brown N. P. , Chenna R. , McGettigan P. A. , McWilliam H. , Valentin F. , Wallace I. M. , Wilm A. et al. & other authors ( 2007; ). Clustal W and Clustal X version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef] [PubMed]
    [Google Scholar]
  19. Lee S. D. . ( 2010; ). Frondihabitans peucedani sp. nov., an actinobacterium isolated from rhizosphere soil, and emended description of the genus Frondihabitans Greene et al. 2009. . Int J Syst Evol Microbiol 60:, 1740–1744. [CrossRef] [PubMed]
    [Google Scholar]
  20. MacKenzie S. L. . ( 1984; ). Amino acids and peptides. In Gas Chromatography/Mass Spectrometry Applications . in Microbiology, pp. 157–204. Edited by Odham G. , Larsson L. , Mardh P. . . New York:: Plenum;.
    [Google Scholar]
  21. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  22. Miller J. A. , Kalyuzhnaya M. G. , Noyes E. , Lara J. C. , Lidstrom M. E. , Chistoserdova L. . ( 2005; ). Labrys methylaminiphilus sp. nov., a novel facultatively methylotrophic bacterium from a freshwater lake sediment. . Int J Syst Evol Microbiol 55:, 1247–1253. [CrossRef] [PubMed]
    [Google Scholar]
  23. Rzhetsky A. , Nei M. . ( 1993; ). Theoretical foundation of the minimum-evolution method of phylogenetic inference. . Mol Biol Evol 10:, 1073–1095.[PubMed]
    [Google Scholar]
  24. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  25. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.
    [Google Scholar]
  26. Schneider T. , Schmid E. , de Castro J. V. Jr , Cardinale M. , Eberl L. , Grube M. , Berg G. , Riedel K. . ( 2011; ). Structure and function of the symbiosis partners of the lung lichen (Lobaria pulmonaria L. Hoffm.) analyzed by metaproteomics. . Proteomics 11:, 2752–2756. [CrossRef] [PubMed]
    [Google Scholar]
  27. Schumann P. , Kämpfer P. , Busse H. J. , Evtushenko L. I. . Subcommittee on the Taxonomy of the Suborder Micrococcineae of the International Committee on Systematics of Prokaryotes ( 2009; ). Proposed minimal standards for describing new genera and species of the suborder Micrococcineae . . Int J Syst Evol Microbiol 59:, 1823–1849. [CrossRef] [PubMed]
    [Google Scholar]
  28. Selbmann L. , Zucconi L. , Ruisi S. , Grube M. , Cardinale M. , Onofri S. . ( 2010; ). Culturable bacteria associated with Antarctic lichens: affiliation and psychrotolerance. . Polar Biol 33:, 71–83. [CrossRef]
    [Google Scholar]
  29. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  30. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  31. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  32. Tindall B. J. , Kämpfer P. , Euzéby J. P. , Oren A. . ( 2006; ). Valid publication of names of prokaryotes according to the rules of nomenclature: past history and current practice. . Int J Syst Evol Microbiol 56:, 2715–2720. [CrossRef] [PubMed]
    [Google Scholar]
  33. Tittsler R. P. , Sandholzer L. A. . ( 1936; ). The use of semi-solid agar for the detection of bacterial motility. . J Bacteriol 31:, 575–580.[PubMed]
    [Google Scholar]
  34. Zhang L. , Xu Z. , Patel B. K. C. . ( 2007; ). Frondicola australicus gen. nov., sp. nov., isolated from decaying leaf litter from a pine forest. . Int J Syst Evol Microbiol 57:, 1177–1182. [CrossRef] [PubMed]
    [Google Scholar]
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