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Abstract

A novel actinobacterium, designated strain CafT13, was isolated from the thallus of the reindeer lichen sampled in the Austrian Alps (Koralpe). The organism was aerobic, with rod- to irregular-shaped cells (often forming dense clusters of cells when grown in liquid medium), Gram-stain-positive, oxidase-negative, catalase-positive and non-motile. It was able to grow at 1 °C and at low to neutral pH, but not above 30 °C or at high pH. The peptidoglycan type was B2β with ornithine as the diagnostic diamino acid. The menaquinones were MK-7 and MK-8. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, three unidentified glycolipids and one unidentified aminolipid. The predominant fatty acids were C, C 2-OH, Cω9, C and anteiso-C. The mean DNA G+C content of strain CafT13 was 69.0±0.17 mol%. 16S rRNA gene sequence analysis showed that strain CafT13 belongs to the family , within the genus . The mean level of DNA–DNA relatedness between strain CafT13 and the type strain of was 35.2±5.23 %. The enzyme spectrum of strain CafT13 differentiated it from recognized species of the genus . Based on molecular, chemotaxonomic and physiological data, strain CafT13 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is CafT13 ( = DSM 23273 = LMG 25550).

Funding
This study was supported by the:
  • Austrian Science Foundation (Award E-1653P19098)
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2011-12-01
2021-05-06
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