1887

Abstract

A novel methanotroph, designated strain E10, was isolated from a rice paddy field in Uruguay. Strain E10 grew on methane and methanol as sole carbon and energy sources. Cells were Gram-negative, non-motile, non-pigmented, slightly curved rods showing type I intracytoplasmic membranes arranged in stacks. The strain was neutrophilic and mesophilic; optimum growth occurred at 30–35 °C with no growth above 37 °C. The strain possessed only a particulate methane monooxygenase (). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain was most closely related to the moderately thermophilic strains OR2 (91.6 % sequence similarity) and Bath (91.5 %). Comparative sequence analysis of genes also confirmed that strain E10 formed a new lineage among the genera and with 89 and 84 % derived amino acid sequence identity to Bath and VKM-14L, respectively. The DNA G+C content was 63.1 mol% and the major cellular fatty acid was C (62.05 %). Thus, strain E10 ( = JCM 16910  = DSM 23452) represents the type strain of a novel species within a new genus, for which the name gen. nov., sp. nov. is proposed.

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2011-11-01
2020-01-24
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vol. , part 11, pp. 2568 - 2572

Neighbour-joining tree based on derived amino acid sequences (148 amino acids) of the pmoA gene of strain E10 and other type I methanotrophs. [PDF](276KB)



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