1887

Abstract

Thirty-one bacteria that belonged to the genus were isolated from cyanobacterial mat samples that were collected from various water bodies in Antarctica. All 31 isolates were psychrophilic; they could be divided into three groups, based on their protein profiles. Representative strains of each of the three groups, namely CMS 35, CMS 38 and CMS 64, were studied in detail. Based on 16S rRNA gene sequence analysis, it was established that the strains were related closely to the group. Phenotypic and chemotaxonomic characteristics further confirmed their affiliation to this group. The three strains could also be differentiated from each other and the closely related species , and , based on phenotypic and chemotaxonomic characteristics and the level of DNA–DNA hybridization. Therefore, it is proposed that strains CMS 35 (=MTCC 4992=DSM 15318), CMS 38 (=MTCC 4993=DSM 15319) and CMS 64 (=MTCC 4994=DSM 15321) should be assigned to novel species of the genus as sp. nov., sp. nov. and sp. nov., respectively.

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2004-05-01
2019-12-08
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References

  1. Anzai, Y., Kim, H., Park, J. Y., Wakabayashi, H. & Oyaizu, H. ( 2000; ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef]
    [Google Scholar]
  2. Baïda, N., Yazourh, A., Singer, E. & Izard, D. ( 2001; ). Pseudomonas brenneri sp. nov., a new species isolated from natural mineral waters. Res Microbiol 152, 493–502.[CrossRef]
    [Google Scholar]
  3. Bradbury, J. F. ( 1987; ). Pseudomonas tolaasi. In CMI Descriptions of Pathogenic Fungi and Bacteria, no. 894. Kew, UK: CAB International Mycological Institute.
  4. Bruni, V., Gugliandolo, C., Maugeri, T. & Allegra, A. ( 1999; ). Psychrotrophic bacteria from a coastal station in the Ross sea (Terra Nova Bay, Antarctica). New Microbiol 22, 357–363.
    [Google Scholar]
  5. Coroler, L., Elomari, M., Hoste, B., Gillis, M., Izard, D. & Leclerc, H. ( 1996; ). Pseudomonas rhodesiae sp. nov., a new species isolated from natural mineral waters. Syst Appl Microbiol 19, 600–607.[CrossRef]
    [Google Scholar]
  6. Dabboussi, F., Hamze, M., Elomari, M., Verhille, S., Baida, N., Izard, D. & Leclerc, H. ( 1999; ). Taxonomic study of bacteria isolated from Lebanese spring waters: proposal for Pseudomonas cedrella sp. nov. and P. orientalis sp. nov. Res Microbiol 150, 303–316.[CrossRef]
    [Google Scholar]
  7. De Ley, J. ( 1992; ). The Proteobacteria: ribosomal RNA cistron similarities and bacterial taxonomy. In The Prokaryotes, 2nd edn, pp. 2111–2140. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. New York: Springer.
  8. Elomari, M., Coroler, L., Hoste, B., Gillis, M., Izard, D. & Leclerc, H. ( 1996; ). DNA relatedness among Pseudomonas strains isolated from natural mineral waters and proposal of Pseudomonas veronii sp. nov. Int J Syst Bacteriol 46, 1138–1144.[CrossRef]
    [Google Scholar]
  9. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Department of Genetics, University of Washington, Seattle, USA.
  10. Higgins, D. G., Bleasby, A. T. & Fuchs, R. ( 1992; ). clustal v: improved software for multiple sequence alignment. Comput Appl Biosci 8, 189–191.
    [Google Scholar]
  11. Holding, A. J. & Collee, J. G. ( 1971; ). Routine biochemical tests. Methods Microbiol 6A, 2–32.
    [Google Scholar]
  12. Hugh, R. & Leifson, E. ( 1953; ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram-negative bacteria. J Bacteriol 66, 24–26.
    [Google Scholar]
  13. Ivanova, E. P., Gorshkova, N. M., Sawabe, T. & 8 other authors ( 2002; ). Pseudomonas extremorientalis sp. nov., isolated from a drinking water reservoir. Int J Syst Evol Microbiol 52, 2113–2120.[CrossRef]
    [Google Scholar]
  14. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  15. Kriss, A. E., Mitskevich, I. N., Rozanova, E. P. & Osnitskaia, L. K. ( 1976; ). Microbiological studies of the Wanda Lake (Antarctica). Mikrobiologiya 45, 1075–1081 (in Russian).
    [Google Scholar]
  16. Laemmli, U. K. ( 1970; ). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef]
    [Google Scholar]
  17. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  18. Maugeri, T. L., Gugliandolo, C. & Bruni, V. ( 1996; ). Heterotrophic bacteria in the Ross Sea (Terra Nova Bay, Antarctica). New Microbiol 19, 67–76.
    [Google Scholar]
  19. Migula, W. ( 1984; ). Über ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe 1, 235–238 (in German).
    [Google Scholar]
  20. Moore, E. R. B., Mau, M., Arnscheidt, A., Böttger, E. C., Hutson, R. A., Collins, M. D., Van De Peer, Y., De Wachter, R. & Timmis, K. N. ( 1996; ). The determination and comparison of the 16S rRNA gene sequences of species of the genus Pseudomonas (sensu stricto) and estimation of the natural intrageneric relationships. Syst Appl Microbiol 19, 478–492.[CrossRef]
    [Google Scholar]
  21. Munsch, P., Alatossava, T., Marttinen, N., Meyer, J.-M., Christen, R. & Gardan, L. ( 2002; ). Pseudomonas costantinii sp. nov., another causal agent of brown blotch disease, isolated from cultivated mushroom sporophores in Finland. Int J Syst Evol Microbiol 52, 1973–1983.[CrossRef]
    [Google Scholar]
  22. Palleroni, N. J., Kunisawa, R., Contopolou, R. & Doudoroff, M. ( 1973; ). Nucleic acid homologies in the genus Pseudomonas. Int J Syst Bacteriol 23, 333–339.[CrossRef]
    [Google Scholar]
  23. Reddy, G. S. N., Aggarwal, R. K., Matsumoto, G. I. & Shivaji, S. ( 2000; ). Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica. Int J Syst Evol Microbiol 50, 1553–1561.[CrossRef]
    [Google Scholar]
  24. Reddy, G. S. N., Prakash, J. S. S., Matsumoto, G. I., Stackebrandt, E. & Shivaji, S. ( 2002; ). Arthrobacter roseus sp. nov., a psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 52, 1017–1021.[CrossRef]
    [Google Scholar]
  25. Reddy, G. S. N., Prakash, J. S. S., Prabahar, V., Matsumoto, G. I., Stackebrandt, E. & Shivaji, S. ( 2003; ). Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 53, 183–187.[CrossRef]
    [Google Scholar]
  26. Sato, N. & Murata, N. ( 1988; ). Membrane lipids. Methods Enzymol 167, 251–259.
    [Google Scholar]
  27. Shinde, P. A. & Lukezic, F. L. ( 1974; ). Isolation, pathogenicity and characterization of fluorescent pseudomonads associated with discoloured alfalfa roots. Phytopathology 64, 865–871.[CrossRef]
    [Google Scholar]
  28. Shivaji, S., Rao, N. S., Saisree, L., Sheth, V., Reddy, G. S. N. & Bhargava, P. M. ( 1989a; ). Isolation and identification of Pseudomonas spp. from Schirmacher Oasis, Antarctica. Appl Environ Microbiol 55, 767–770.
    [Google Scholar]
  29. Shivaji, S., Rao, N. S., Saisree, L., Reddy, G. S. N., Seshu Kumar, G. & Bhargava, P. M. ( 1989b; ). Isolates of Arthrobacter from the soils of Schirmacher Oasis, Antarctica. Polar Biol 10, 225–229.
    [Google Scholar]
  30. Shivaji, S., Ray, M. K., Rao, N. S., Saisree, L., Jagannadham, M. V., Seshu Kumar, G., Reddy, G. S. N. & Bhargava, P. M. ( 1992; ). Sphingobacterium antarcticus sp. nov., a psychrotrophic bacterium from the soils of Schirmacher Oasis, Antarctica. Int J Syst Bacteriol 42, 102–106.[CrossRef]
    [Google Scholar]
  31. Shivaji, S., Vijaya Bhanu, N. & Aggarwal, R. K. ( 2000; ). Identification of Yersinia pestis as the causative organism of plague in India as determined by 16S rDNA sequencing and RAPD-based genomic fingerprinting. FEMS Microbiol Lett 189, 247–252.[CrossRef]
    [Google Scholar]
  32. Stanier, R. Y., Palleroni, N. J. & Doudoroff, M. ( 1966; ). The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43, 159–271.[CrossRef]
    [Google Scholar]
  33. Stolp, H. & Gadkari, D. ( 1981; ). Nonpathogenic members of the genus Pseudomonas. In The Prokaryotes, vol. 1, pp. 719–741. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. Berlin: Springer.
  34. Tourova, T. P. & Antonov, A. S. ( 1987; ). Identification of microorganisms by rapid DNA-DNA hybridisation. Methods Microbiol 19, 333–355.
    [Google Scholar]
  35. Verhille, S., Baïda, N., Dabboussi, F., Hamze, M., Izard, D. & Leclerc, H. ( 1999; ). Pseudomonas gessardii sp. nov. and Pseudomonas migulae sp. nov., two new species isolated from natural mineral waters. Int J Syst Bacteriol 49, 1559–1572.[CrossRef]
    [Google Scholar]
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vol. , part 3, pp. 713 – 719

Comparison of the fatty acid composition of strains CMS 35 ( ), CMS 38 ( ) and CMS 64 ( ) with closely related species of the genus .

DNA–DNA relatedness of CMS 35 , CMS 38 and CMS 64 with closely related species.

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