1887

Abstract

A novel actinomycete producing 2-allyloxyphenol, designated strain MS1/7, was isolated from sediments of the Sundarbans mangrove forest, India. Growth on International Project (ISP) media 2, 3, 4, 5 and 7 produced olive green to grey aerial hyphae that carried smooth-surfaced spores in a flexuous () arrangement. The strain contained -diaminopimelic acid, but no diagnostic sugars in whole-cell hydrolysates. Hexa-, octa- and a minor amount of tetra-hydrogenated menaquinones with nine isoprene units [MK-9 (H, H, H and H)] were present as isoprene analogues. Diagnostic phospholipids were phosphatidylethanolamine and diphosphatidylglycerol. The predominant fatty acids were anteiso-C (34.80 %), iso-C (16.45 %), C (10.53 %) and anteiso-C (10.92 %). The strain showed greater than 99 % 16S rRNA gene sequence similarity to the type strains of several recognized species of the genus , but in the phylogenetic tree based on 16S rRNA gene sequences it formed a distinct phyletic line and demonstrated closest relationships to viomycin-producers ( NRRL B-1221, MTCC 2534 and NRRL B-2895). However, strain MS1/7 could be distinguished from these and other closely related species based on low levels of DNA–DNA relatedness (<44 %) and disparate physiological features, principally amino acid utilization and growth in NaCl. Strain MS1/7 is therefore suggested to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MS1/7 ( = MTCC 10621 = DSM 42019).

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2011-11-01
2019-10-23
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