1887

Abstract

Apple proliferation (AP), pear decline (PD) and European stone fruit yellows (ESFY) are among the most economically important plant diseases that are caused by phytoplasmas. Phylogenetic analyses revealed that the 16S rDNA sequences of strains of each of these pathogens were identical or nearly identical. Differences between the three phytoplasmas ranged from 1·0 to 1·5 % of nucleotide positions and were thus below the recommended threshold of 2·5 % for assigning species rank to phytoplasmas under the provisional status ‘’. However, supporting data for distinguishing the AP, PD and ESFY agents at the species level were obtained by examining other molecular markers, including the 16S–23S rDNA spacer region, protein-encoding genes and randomly cloned DNA fragments. The three phytoplasmas also differed in serological comparisons and showed clear differences in vector transmission and host-range specificity. From these results, it can be concluded that the AP, PD and ESFY phytoplasmas are coherent but discrete taxa that can be distinguished at the putative species level, for which the names ‘ Phytoplasma mali’, ‘ Phytoplasma pyri’ and ‘ Phytoplasma prunorum’, respectively, are proposed. Strains AP15, PD1 and ESFY-G1 were selected as reference strains. Examination of available data on the peach yellow leaf roll (PYLR) phytoplasma, which clusters with the AP, PD and ESFY agents, confirmed previous results showing that it is related most closely to the PD pathogen. The two phytoplasmas share 99·6 % 16S rDNA sequence similarity. Significant differences were only observed in the sequence of a gene that encodes an immunodominant membrane protein. Until more information on this phytoplasma is available, it is proposed that the PYLR phytoplasma should be regarded as a subtype of ‘ Phytoplasma pyri’.

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2004-07-01
2019-10-17
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References

  1. Ahrens, U., Lorenz, K.-H. & Seemüller, E. ( 1993; ). Genetic diversity among mycoplasmalike organisms associated with stone fruit diseases. Mol Plant–Microbe Interact 6, 686–691.[CrossRef]
    [Google Scholar]
  2. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  3. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. ( 1987; ). Current Protocols in Molecular Biology. New York: Wiley.
  4. Barbara, D. J., Morton, A., Clark, M. F. & Davies, D. L. ( 2001; ). Molecular variation in immunodominant membrane proteins from phytoplasmas. Acta Hortic 550, 405–408.
    [Google Scholar]
  5. Barbara, D. J., Morton, A., Clark, M. F. & Davies, D. L. ( 2002; ). Immunodominant membrane proteins from two phytoplasmas in the aster yellows clade (chlorante aster yellows and clover phyllody) are highly divergent in the major hydrophilic region. Microbiology 148, 157–167.
    [Google Scholar]
  6. Berg, M. ( 1998; ). Elongationsfaktoren und immunogene Membranproteine des Apfeltriebsucht-Phytoplasmas. PhD thesis, Technische Universität Darmstadt, Germany (in German).
  7. Berg, M., Davies, D. L., Clark, M. F., Vetten, H. J., Maier, G., Marcone, C. & Seemüller, E. ( 1999; ). Isolation of the gene encoding an immunodominant membrane protein of the apple proliferation phytoplasma, and expression and characterization of the gene product. Microbiology 145, 1937–1943.[CrossRef]
    [Google Scholar]
  8. Berges, R. ( 1999; ). Untersuchungen zur molekularbiologischen Identifizierung und ätiologischen Bedeutung von Phytoplasmen in Wald- und Feldgehölzen und zur Quantifizierung der Erreger in Wirtspflanzen mit unterschiedlicher Besiedlungsdichte. PhD thesis, Universität Heidelberg, Germany (in German).
  9. Blomquist, C. L. & Kirkpatrick, B. C. ( 2002; ). Identification of phytoplasma taxa and insect vectors of peach yellow leaf roll disease in California. Plant Dis 86, 759–763.[CrossRef]
    [Google Scholar]
  10. Bonnet, F., Saillard, C., Kollar, A., Seemüller, E. & Bové, J. M. ( 1990; ). Detection and differentiation of the mycoplasmalike organism associated with apple proliferation disease using cloned DNA probes. Mol Plant–Microbe Interact 3, 438–443.[CrossRef]
    [Google Scholar]
  11. Bradbury, J. M., Abdul-Wahab, O. M. S., Yavari, C. A., Dupiellet, J.-P. & Bové, J. M. ( 1993; ). Mycoplasma imitans sp. nov. is related to Mycoplasma gallisepticum and found in birds. Int J Syst Bacteriol 43, 721–728.[CrossRef]
    [Google Scholar]
  12. Carraro, L., Osler, R., Refatti, E. & Poggi Pollini, C. ( 1988; ). Transmission of the possible agent of apple proliferation to Vinca rosea by dodder. Riv Patol Veg 24, 43–52.
    [Google Scholar]
  13. Carraro, L., Loi, N., Ermacora, P., Gregoris, A. & Osler, R. ( 1998a; ). Transmission of pear decline by using naturally infected Cacopsylla pyri L. Acta Hortic 472, 665–668.
    [Google Scholar]
  14. Carraro, L., Osler, R., Loi, N., Ermacora, P. & Refatti, E. ( 1998b; ). Transmission of European stone fruit yellows phytoplasma by Cacopsylla pruni. J Plant Pathol 80, 233–239.
    [Google Scholar]
  15. Carraro, L., Ferrini, F., Ermacora, P. & Loi, N. ( 2002; ). Role of wild Prunus species in the epidemiology of European stone fruit yellows. Plant Pathol 51, 513–517.[CrossRef]
    [Google Scholar]
  16. Christiansen, G. ( 1992; ). Genetic variation in natural populations. In Mycoplasmas: Molecular Biology and Pathogenesis, pp. 561–573. Edited by J. Maniloff, R. N. McElhaney, L. R. Finch & J. B. Baseman. Washington, DC: American Society for Microbiology.
  17. Davies, D. L. & Adams, A. N. ( 2001; ). European stone fruit yellows phytoplasmas in southern England. Acta Hortic 550, 389–393.
    [Google Scholar]
  18. Del Serrone, P., La Starza, S., Krystai, L., Kölber, M. & Barba, M. ( 1998; ). Occurrence of apple proliferation and pear decline phytoplasmas in diseased pear trees in Hungary. J Plant Pathol 80, 53–58.
    [Google Scholar]
  19. Deng, S. & Hiruki, C. ( 1991; ). Amplification of 16S rRNA genes from culturable and nonculturable mollicutes. J Microbiol Methods 14, 53–61.[CrossRef]
    [Google Scholar]
  20. Frisinghelli, C., Delaiti, L., Grando, M. S., Forti, D. & Vindimian, M. E. ( 2000; ). Cacopsylla costalis (Flor 1861), as a vector of apple proliferation in Trentino. J Phytopathol 148, 425–431.[CrossRef]
    [Google Scholar]
  21. Goidanich, G. ( 1933; ). Un deperimento dei susini. Boll Stn Patol Veg Roma 13, 160–173 (in Italian).
    [Google Scholar]
  22. Griffiths, H. M., Sinclair, W. A., Smart, C. D. & Davis, R. E. ( 1999; ). The phytoplasma associated with ash yellows and lilac witches’-broom: ‘Candidatus Phytoplasma fraxini’. Int J Syst Bacteriol 49, 1605–1614.[CrossRef]
    [Google Scholar]
  23. Guerra, L. J. ( 1997; ). Biological and molecular characterization of phytoplasmas infecting fruit and nut trees in California. PhD thesis, University of California, CA, USA.
  24. Heldtander, M., Pettersson, B., Tully, J. G. & Johansson, K.-E. ( 1998; ). Sequences of the 16S rRNA genes and phylogeny of the goat mycoplasmas Mycoplasma adleri, Mycoplasma auris, Mycoplasma cottewii and Mycoplasma yeatsii. Int J Syst Bacteriol 48, 263–268.[CrossRef]
    [Google Scholar]
  25. IRPCM Phytoplasma/Spiroplasma Working Team – Phytoplasma taxonomy group ( 2004; ).Candidatus Phytoplasma’, a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects. Int J Syst Evol Microbiol 54, 1257–1269.[CrossRef]
    [Google Scholar]
  26. Jarausch, W., Saillard, C., Dosba, F. & Bové, J.-M. ( 1994; ). Differentiation of mycoplasmalike organisms (MLOs) in European fruit trees by PCR using specific primers derived from the sequence of a chromosomal fragment of the apple proliferation MLO. Appl Environ Microbiol 60, 2916–2923.
    [Google Scholar]
  27. Jarausch, W., Saillard, C., Broquaire, J. M., Garnier, M. & Dosba, F. ( 2000a; ). PCR-RFLP and sequence analysis of a non-ribosomal fragment for genetic characterization of European stone fruit yellows phytoplasmas infecting various Prunus species. Mol Cell Probes 14, 171–179.[CrossRef]
    [Google Scholar]
  28. Jarausch, W., Saillard, C., Helliot, B., Garnier, M. & Dosba, F. ( 2000b; ). Genetic variability of apple proliferation phytoplasmas as determined by PCR-RFLP and sequencing of a non-ribosomal fragment. Mol Cell Probes 14, 17–24.[CrossRef]
    [Google Scholar]
  29. Jarausch, W., Jarausch-Wehrheim, B., Danet, J. L., Broquaire, J. M., Dosba, F., Saillard, C. & Garnier, M. ( 2001; ). Detection and identification of European stone fruit yellows and other phytoplasmas in wild plants in the surroundings of apricot chlororic leaf roll-affected orchards in southern France. Eur J Plant Pathol 107, 209–217.[CrossRef]
    [Google Scholar]
  30. Jensen, D. D., Griggs, W. H., Gonzales, C. Q. & Schneider, H. ( 1964; ). Pear decline virus transmission by pear psylla. Phytopathology 54, 1346–1351.
    [Google Scholar]
  31. Kartte, S. & Seemüller, E. ( 1991; ). Susceptibility of grafted Malus taxa and hybrids to apple proliferation disease. J Phytopathol 131, 137–148.[CrossRef]
    [Google Scholar]
  32. Kirkpatrick, B. C., Smart, C. D., Gardner, S. & 9 other authors ( 1994; ). Phylogenetic relationship of plant pathogenic MLOs established by 16/23S rDNA spacer sequences. IOM Lett 3, 228–229.
    [Google Scholar]
  33. Kison, H. & Seemüller, E. ( 2001; ). Differences in strain virulence of the European stone fruit yellows phytoplasma and susceptibility of stone fruit trees on various rootstocks to this pathogen. J Phytopathol 149, 533–541.[CrossRef]
    [Google Scholar]
  34. Kison, H., Schneider, B. & Seemüller, E. ( 1994; ). Restriction fragment length polymorphism within the apple proliferation mycoplasmalike organism. J Phytopathol 141, 395–401.[CrossRef]
    [Google Scholar]
  35. Kison, H., Kirkpatrick, B. C. & Seemüller, E. ( 1997; ). Genetic comparison of the peach yellow leaf roll agent with European fruit tree phytoplasmas of the apple proliferation group. Plant Pathol 46, 538–544.[CrossRef]
    [Google Scholar]
  36. Kollar, A. & Seemüller, E. ( 1989; ). Base composition of the DNA of mycoplasmalike organisms associated with various plant diseases. J Phytopathol 127, 177–186.[CrossRef]
    [Google Scholar]
  37. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetic analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  38. Lauer, U. & Seemüller, E. ( 2000; ). Physical map of the chromosome of the apple proliferation phytoplasma. J Bacteriol 182, 1415–1418.[CrossRef]
    [Google Scholar]
  39. Lee, I.-M., Davis, R. E., Chen, T. A., Chiykowski, L. N., Fletcher, J., Hiruki, C. & Schaff, D. A. ( 1992; ). A genotype-based system for identification and classification of mycoplasmalike organisms (MLOs) in the aster yellows MLO strain cluster. Phytopathology 82, 977–986.[CrossRef]
    [Google Scholar]
  40. Lee, I.-M., Bertaccini, A., Vibio, M. & Gundersen, D. E. ( 1995; ). Detection of multiple phytoplasmas in perennial fruit trees with decline symptoms in Italy. Phytopathology 85, 728–735.[CrossRef]
    [Google Scholar]
  41. Lee, I.-M., Davis, R. E. & Gundersen-Rindal, D. E. ( 2000; ). Phytoplasma: phytopathogenic mollicutes. Annu Rev Microbiol 54, 221–255.[CrossRef]
    [Google Scholar]
  42. Lim, P.-O. & Sears, B. B. ( 1989; ). 16S rRNA sequence indicates that plant-pathogenic mycoplasmalike organisms are evolutionarily distinct from animal mycoplasmas. J Bacteriol 171, 5901–5906.
    [Google Scholar]
  43. Loi, N., Carraro, L., Musetti, R., Pertot, I. & Osler, R. ( 1995; ). Dodder transmission of two different MLOs from plum trees affected by “leptonecrosis”. Acta Hortic 386, 465–470.
    [Google Scholar]
  44. Loi, N., Ermacora, P., Carraro, L., Osler, R. & Chen, T. A. ( 2002; ). Production of monoclonal antibodies against apple proliferation phytoplasma and their use in serological detection. Eur J Plant Pathol 108, 81–86.[CrossRef]
    [Google Scholar]
  45. Lorenz, K.-H., Dosba, F., Poggi Pollini, C., Llacer, G. & Seemüller, E. ( 1994; ). Phytoplasma diseases of Prunus species in Europe are caused by genetically similar organisms. Z Pflanzenkr Pflanzenschutz 101, 567–575.
    [Google Scholar]
  46. Lorenz, K.-H., Schneider, B., Ahrens, U. & Seemüller, E. ( 1995; ). Detection of the apple proliferation and pear decline phytoplasmas by PCR amplification of ribosomal and nonribosomal DNA. Phytopathology 85, 771–776.[CrossRef]
    [Google Scholar]
  47. Marcone, C. & Seemüller, E. ( 2001; ). A chromosome map of the European stone fruit yellows phytoplasma. Microbiology 147, 1213–1221.
    [Google Scholar]
  48. Marcone, C., Ragozzino, A., Del Serrone, P., Aloj, B., Barba, M. & Seemüller, E. ( 1996a; ). Detection of apple proliferation and pear decline in southern Italy. Petria 6, 149–157.
    [Google Scholar]
  49. Marcone, C., Ragozzino, A., Schneider, B., Lauer, U., Smart, C. D. & Seemüller, E. ( 1996b; ). Genetic characterization and classification of two phytoplasmas associated with spartium witches’-broom disease. Plant Dis 80, 365–371.[CrossRef]
    [Google Scholar]
  50. Marcone, C., Ragozzino, A. & Seemüller, E. ( 1996c; ). Association of phytoplasmas with the decline of European hazel in southern Italy. Plant Pathol (Oxf) 45, 857–863.[CrossRef]
    [Google Scholar]
  51. Marcone, C., Hergenhahn, F., Ragozzino, A. & Seemüller, E. ( 1999a; ). Dodder transmission of pear decline, European stone fruit yellows, rubus stunt, picris echioides yellows and cotton phyllody phytoplasmas to periwinkle. J Phytopathol 147, 187–192.[CrossRef]
    [Google Scholar]
  52. Marcone, C., Neimark, H., Ragozzino, A., Lauer, U. & Seemüller, E. ( 1999b; ). Chromosome sizes of phytoplasmas composing major phylogenetic groups and subgroups. Phytopathology 89, 805–810.[CrossRef]
    [Google Scholar]
  53. Marwitz, R., Petzold, H. & Özel, M. ( 1974; ). Untersuchungen zur Übertragbarkeit des möglichen Erregers der Triebsucht des Apfels auf einen krautigen Wirt. Phytopathol Z 81, 85–91 (in German).[CrossRef]
    [Google Scholar]
  54. Mäurer, R. & Seemüller, E. ( 1996; ). Witches' broom of Rhamnus catharticus: a new phytoplasma disease. J Phytopathol 144, 221–223.[CrossRef]
    [Google Scholar]
  55. McLarty, H. R. ( 1948; ). Killing of pear trees. Ann Rep Canad Plant Dis Surv 28, 77.
    [Google Scholar]
  56. Morton, A., Davies, D. L., Blomquist, C. L. & Barbara, D. J. ( 2003; ). Characterization of homologues of the apple proliferation immunodominant membrane protein gene from three related phytoplasmas. Mol Plant Pathol 4, 109–114.[CrossRef]
    [Google Scholar]
  57. Murray, R. G. E. & Schleifer, K. H. ( 1994; ). Taxonomic notes: a proposal for recording the properties of putative taxa of procaryotes. Int J Syst Bacteriol 44, 174–176.[CrossRef]
    [Google Scholar]
  58. Murray, R. G. E. & Stackebrandt, E. ( 1995; ). Taxonomic note: implementation of the provisional status Candidatus for incompletely described procaryotes. Int J Syst Bacteriol 45, 186–187.[CrossRef]
    [Google Scholar]
  59. Rui, D., Ciferri, R. & Refatti, E. ( 1950; ). La virosi degli “scopazzi del melo” nel Veronese. Not Mal Piante 13, 7–11 (in Italian).
    [Google Scholar]
  60. Schneider, B. & Seemüller, E. ( 1994a; ). Presence of two sets of ribosomal genes in phytopathogenic mollicutes. Appl Environ Microbiol 60, 3409–3412.
    [Google Scholar]
  61. Schneider, B. & Seemüller, E. ( 1994b; ). Studies on the taxonomic relationships of mycoplasmalike organisms by Southern blot analysis. J Phytopathol 141, 173–185.[CrossRef]
    [Google Scholar]
  62. Schneider, B., Seemüller, E., Smart, C. D. & Kirkpatrick, B. C. ( 1995; ). Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas. In Molecular and Diagnostic Procedures in Mycoplasmology, vol. 1, pp. 369–380. Edited by S. Razin & J. G. Tully. San Diego, CA: Academic Press.
  63. Schneider, B., Marcone, C., Kampmann, M., Ragozzino, A., Lederer, W., Cousin, M.-T. & Seemüller, E. ( 1997; ). Characterization and classification of phytoplasmas from wild and cultivated plants by RFLP and sequence analysis of ribosomal DNA. Eur J Plant Pathol 103, 675–686.[CrossRef]
    [Google Scholar]
  64. Seemüller, E. ( 1992; ). Pear decline. In Plant Diseases of International Importance: Diseases of Fruit Crops, pp. 308–334. Edited by J. Kumar, U. S. Singh, A. N. Mukhopadhyay & H. S. Chaube. Englewood Cliffs, NJ: Prentice Hall.
  65. Seemüller, E. ( 1999; ). On some problems in temperate fruit tree phytoplasma research. In First Internet Conference on Phytopathogenic Mollicutes (http://www.Uniud.It/phytoplasma/pap/seem2700.html).
  66. Seemüller, E. ( 2002; ). Apple proliferation: etiology, epidemiology and detection. In Atti Giornata Fitopatologiche, pp. 3–6. Baselga di Piné (Trento), 7–11 April 2002.
  67. Seemüller, E., Schneider, B., Mäurer, R. & 8 other authors ( 1994; ). Phylogenetic classification of phytopathogenic mollicutes by sequence analysis of 16S ribosomal DNA. Int J Syst Bacteriol 44, 440–446.[CrossRef]
    [Google Scholar]
  68. Seemüller, E., Kison, H., Lorenz, K.-H., Schneider, B., Marcone, C., Smart, C. D. & Kirkpatrick, B. C. ( 1998a; ). Detection and identification of fruit tree phytoplasmas by PCR amplification of ribosomal and nonribosomal DNA. In COST 823: New Technologies to Improve Phytodiagnosis. Advances in the Detection of Plant Pathogens by Polymerase Chain Reaction, pp. 56–66. Edited by C. Manceau. Luxembourg: European Community.
  69. Seemüller, E., Marcone, C., Lauer, U., Ragozzino, A. & Göschl, M. ( 1998b; ). Current status of molecular classification of the phytoplasmas. J Plant Pathol 80, 3–26.
    [Google Scholar]
  70. Seemüller, E., Garnier, M. & Schneider, B. ( 2002; ). Mycoplasmas of plants and insects. In Molecular Biology and Pathology of Mycoplasmas, pp. 91–116. Edited by S. Razin & R. Herrmann. London: Kluwer Academic/Plenum Publishers.
  71. Shalla, T. A., Chiarappa, L., Blodgett, E. C., Refatti, E. & Baldacci, E. ( 1961; ). The probable coidentity of the moria disease of pear in Italy and pear decline in North America. Plant Dis Rep 45, 912–915.
    [Google Scholar]
  72. Tedeschi, R., Bosco, D. & Alma, A. ( 2002; ). Population dynamics of Cacopsylla melanoneura (Homoptera: Psyllidae), a vector of apple proliferation phytoplasma in northwestern Italy. J Econ Entomol 95, 544–551.[CrossRef]
    [Google Scholar]
  73. Topchiiska, M., Marcone, C. & Seemüller, E. ( 2000; ). Detection of pear decline and European stone fruit yellows in Bulgaria. Z Pflanzenkr Pflanzenschutz 107, 658–663.
    [Google Scholar]
  74. Woodbridge, C. G., Blodgett, E. C. & Diener, T. O. ( 1957; ). Pear decline in the Pacific Northwest. Plant Dis Rep 41, 569–572.
    [Google Scholar]
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vol. , part 4, pp. 1217 – 1226

Restriction maps of rDNA obtained from P1/P7 PCR products from the apple proliferation, pear decline and European stone fruit yellows phytoplasmas [PDF](161 KB).



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