Characterization of the marine propionate-degrading, sulfate-reducing bacterium Desulfofaba fastidiosa sp. nov. and reclassification of Desulfomusa hansenii as Desulfofaba hansenii comb. nov.
A rod-shaped, slightly curved sulfate reducer, designated strain P2T, was isolated from the sulfate–methane transition zone of a marine sediment. Cells were motile by means of a single polar flagellum. The strain reduced sulfate, thiosulfate and sulfite to sulfide and used propionate, lactate and 1-propanol as electron donors. Strain P2T also grew by fermentation of lactate. Propionate was oxidized incompletely to acetate and CO2. The DNA G+C content was 48·8mol%. Sequence analysis of the small-subunit rDNA and the dissimilatory sulfite reductase gene revealed that strain P2T was related to the genera Desulfonema, Desulfococcus, Desulfosarcina, ‘Desulfobotulus’, Desulfofaba, Desulfomusa and Desulfofrigus. These genera include incomplete as well as complete oxidizers of substrates. Strain P2T shared important morphological and physiological traits with Desulfofaba gelida and Desulfomusa hansenii, including the ability to oxidize propionate incompletely to acetate. The 16S rRNA gene similarities of P2T to Desulfofaba gelida and Desulfomusa hansenii were respectively 92·9 and 91·5%. Combining phenotypic and genotypic traits, we propose strain P2T to be a member of the genus Desulfofaba. The name Desulfofaba fastidiosa sp. nov. (type strain P2T=DSM 15249T=ATCC BAA-815T) is proposed, reflecting the limited number of substrates consumed by the strain. In addition, the reclassification of Desulfomusa hansenii as a member of the genus Desulfofaba, Desulfofaba hansenii comb. nov., is proposed. A common line of descent and a number of shared phenotypic traits support this reclassification.
BrosiusJ.,
DullT. J.,
SleeterD. D.,
NollerH. F.1981; Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli
. J Mol Biol 148:107–127[CrossRef]
CashionP.,
Holder-FranklinM. A.,
McCullyJ.,
FranklinM.1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466[CrossRef]
DevereuxR.,
HeS.-H.,
DoyleC. L.,
OrklandS.,
StahlD. A.,
LeGallJ.,
WhitmanW. B.1990; Diversity and origin of Desulfovibrio species: phylogenetic definition of a family. J Bacteriol 172:3609–3619
IsaksenM. F.,
FinsterK.1996; Sulphate reduction in the root zone of the seagrass Zostera noltii on the intertidal flats of a coastal lagoon (Arcachon, France). Mar Ecol Prog Ser 137:187–194[CrossRef]
IsaksenM. F.,
TeskeA.1996; Desulforhopalus vacuolatus gen. nov., sp. nov. a new moderately psychrophilic sulfate-reducing bacterium with gas vacuoles isolated from a temperate estuary. Arch Microbiol 166:160–168[CrossRef]
JanssenP. H.,
SchinkB.1995a; Catabolic and anabolic enzyme activities and energetics of acetone metabolism of the sulfate-reducing bacterium Desulfococcus biacutus
. J Bacteriol 177:277–282
LaneD. J.,
PaceB.,
OlsenG. J.,
StahlD. A.,
SoginM. L.,
PaceN. R.1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82:6955–6959[CrossRef]
MesbahM.,
PremachandranU.,
WhitmanW.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167[CrossRef]
ParkesR. J.,
DowlingN. J. E.,
WhiteD. C.,
HerbertR. A.,
GibsonG. R.1993; Characterization of sulphate-reducing bacterial populations within marine and estuarine sediments with different rates of sulphate reduction. FEMS Microbiol Ecol 102:235–250[CrossRef]
PostgateJ. R.1984; Genus Desulfovibrio Kluyver and van Niel 1936, 397AL
. In Bergey's Manual of Systematic Bacteriologyvol. 1 p. 666–672 Edited by
KriegN. R.,
HoltJ. G.
Baltimore: William & Wilkins;
RabusR.,
HansenT.,
WiddelF.2000; Dissimilatory sulfate- and sulfur-reducing prokaryotes. In The Prokaryotes release 3–3 New York: Springer; http://www.prokaryotes.com
ReesG. N.,
PatelB. K. C.2001; Desulforegula conservatrix gen. nov., sp. nov., a long-chain fatty acid-oxidizing, sulfate-reducing bacterium isolated from sediments of a freshwater lake. Int J Syst Evol Microbiol 51:1911–1916[CrossRef]
StamsA. J. M.,
VeenhuisM.,
WenkG. H.,
HansenT. A.1983; Occurrence of polyglucose as a storage polymer in Desulfovibrio species and Desulfobulbus propionicus
. Arch Microbiol 136:54–59[CrossRef]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128[CrossRef]
VisuvanathanS.,
MossM. T.,
StandordJ. L.,
Hermon-TaylorJ.,
McFaddenJ. J.1989; Simple enzymatic method for isolation of DNA from diverse bacteria. J Microbiol Methods 10:59–64[CrossRef]
WagnerM.,
RogerA. J.,
FlaxJ. L.,
BrusseauG. A.,
StahlD. A.1998; Phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration. J Bacteriol 180:2975–2982
WheelerD. L.,
ChurchD. M.,
LashA. E.8 other authors2002; Database resources of the National Center for Biotechnology Information: 2002 update. Nucleic Acids Res 30:13–16[CrossRef]
WiddelF.,
PfennigN.1982; Studies on dissimilatory sulphate-reducing bacteria that decompose fatty acids. II. Incomplete oxidation of propionate by Desulfobulbus propionicus gen. nov., sp. nov. Arch Microbiol 31:360–365
WiddelF.,
PfennigN.1984; Dissimilatory sulfate- or sulfur-reducing bacteria. In Bergey's Manual of Systematic Bacteriologyvol. 1p– 663 Edited by
KriegN. R.,
HoltJ. G.
Baltimore: William & Wilkins;
Characterization of the marine propionate-degrading, sulfate-reducing bacterium Desulfofaba fastidiosa sp. nov. and reclassification of Desulfomusa hansenii as Desulfofaba hansenii comb. nov.