1887

Abstract

A novel, Gram-positive, rod-shaped, motile, endospore-forming, halophilic bacterial strain, J18, was isolated from a traditional salt-fermented seafood made of gizzard shad in Korea. Colonies were convex, cream-coloured and 1.0–2.0 mm in diameter after incubation for 3 days on marine agar. Growth occurred at pH 7.0–11.0 (optimum, pH 10.0), at 4–40 °C (optimum, 37 °C) and in the presence of 0–30 % NaCl (optimum, 9–10 %). On the basis of 16S rRNA gene sequence analysis, strain J18 was related most closely to ISL-24 (96.3 % similarity), LMG 20964 (96.2 %), DSM 10037 (96.0 %), Hal 1 (95.5 %) and LMG 19488 (95.5 %). The major fatty acids were anteiso-C and anteiso-C. The DNA G+C content of strain J18 was 37.0 mol%. The cell-wall peptidoglycan was of the -diaminopimelic acid type. The major quinone was menaquinone 7 (MK-7). Based on phenotypic, chemotaxonomic and phylogenetic data, strain J18 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is J18 ( = KACC 14624  = JCM 16994).

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2011-12-01
2019-12-06
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vol. , part 12, pp. 2851 - 2855

Two-dimensional thin-layer chromatograms of the polar lipids from strain J18 . Phospholipids and total polar lipids were detected with Zinzadze reagent (a) and molybdatophosphoric acid (b). DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; L1, L2, unknown lipids.



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