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Volume 54, Issue 6

sp. nov. and sp. nov., isolated from diverse environments, and emended descriptions of the genus Finkmann . 2000 and of its type species Free

Abstract

Three mesophilic bacteria (strains AMX 26B, UR374_02 and 12-3) isolated respectively from an anaerobic digester, human urine and urban riverside soil were characterized. Cells were Gram-negative, motile, non-sporulating, straight to curved rods with one polar flagellum and had a strictly respiratory metabolism with O as the preferential terminal electron acceptor. Phylogenetic analysis based on 16S rRNA gene sequences revealed that all strains clustered within the branch of the . Isolates AMX 26B and UR374_02 exhibited 100 % 16S rRNA gene sequence similarity and both were related to strain 12-3 (99·6 % similarity). The closest relative of all the isolates was DSM 12573 (similarity 97·1–97·5 %), and they were equidistantly related to species (95·4–96·6 %), species (95·3–96·1 %) and ATCC BAA-4040 (95·3–95·4 %). Chemotaxonomic and biochemical data (branched-chain cellular fatty acid pattern without C iso 3-OH, ubiquinone with eight isoprenoid units, limited range of substrates used, ability to reduce nitrite but not nitrate with the production of NO) supported their affiliation to the genus . The results of DNA–DNA hybridization and/or phenotypic analysis allowed them to be differentiated from the two species with validly published names and showed that strain 12-3 was genomically and phenotypically distinct from the other two isolates. On the basis of these results, two novel species of the genus are proposed: sp. nov., consisting of strains AMX 26B (=ATCC 700993=CIP 106674=JCM 11524) (type strain) and UR374_02 (=DSM 15133), and sp. nov., consisting of strain 12-3 (=CCUG 48231=CIP 107388=JCM 11525). The report of these two novel species leads to the emendation of the description of the genus and the re-evaluation of the phenotype of DSM 12573 necessitates the emendation of its description.

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Keyword(s): CFA, cellular fatty acids
SGM

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sp. nov. and sp. nov., isolated from diverse environments, and emended descriptions of the genus Finkmann . 2000 and of its type species
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Pseudoxanthomonas mexicana sp. nov. and Pseudoxanthomonas japonensis sp. nov., isolated from diverse environments, and emended descriptions of the genus Pseudoxanthomonas Finkmann et al. 2000 and of its type species
Int J Syst Evol Microbiol 54, 2245 (2004); https://doi.org/10.1099/ijs.0.02810-0
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