1887

Abstract

A bacterial strain designated XIL08 was isolated from an elm tree affected by Dutch elm disease. Strain XIL08 is Gram-positive, aerobic, rod-shaped and non-motile. The complete 16S rDNA sequence of this micro-organism was obtained and phylogenetic analysis based on the neighbour-joining method indicated that the closest related organism belongs to the genus of the family , suborder . Cell-wall analyses revealed the presence of type A4, -lys–-ala–-Glu peptidoglycan. The cell-wall sugars found were rhamnose in large amounts, fucose, mannose and galactose and traces of arabinose and glucose. HPLC analysis of menaquinones revealed two peaks, the main peak corresponding to MK-9(H) and the smaller one to MK-8(H). The major fatty acid found was anteiso-C. Mycolic acids were absent. The polar lipids detected were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The G+C content of the DNA was 72 mol%. Isolate XIL08 hydrolysed xylan but not cellulose. Growth was observed with many carbohydrates including acetate and xylan as the only carbon source. Catalase activity was not detected. The data from this polyphasic study suggest that this bacterium belongs to a novel genus of the family . It is proposed that isolate XIL08 (=LMG 21721=CECT 5731) be classified in a new genus, gen. nov., as the type strain of sp. nov.

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2004-03-01
2024-11-12
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References

  1. Bakalidou A., Kämpfer P., Berchtold M., Kuhnigk T., Wenzel M., König H. 2002; Cellulosimicrobium variabile sp. nov., a cellulolytic bacterium from the hindgut of the termite Mastotermes darwiniensis . Int J Syst Evol Microbiol 52:1185–1192 [CrossRef]
    [Google Scholar]
  2. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S gene rRNA sequences. Int J Syst Bacteriol 45:240–245 [CrossRef]
    [Google Scholar]
  3. Doetsch R. N. 1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology pp  21–33 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  4. Evtushenko L. I., Levanova G. F., Agre N. S. 1984; Nucleotide composition of DNA and amino acid composition of A4 peptidoglycan in Promicromonospora citrea . Mikrobiologiya 53:519–520
    [Google Scholar]
  5. Goodfellow M. 1989; Suprageneric classification of actinomycetes. In Bergey's Manual of Systematic Bacteriology vol 4 pp  2333–2339 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  6. Kalakoutskii L. V., Agre N. S., Prauser H., Evtushenko L. I. 1989; Genus Promicromonospora Krasil'nikov, Kalakoutskii and Kirillova 1961a, 107AL . In Bergey's Manual of Systematic Bacteriology vol 4 pp  2392–2395 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  7. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  8. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  9. Mackenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N -heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160
    [Google Scholar]
  10. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  11. Rivas R., Sánchez M., Trujillo M. E., Zurdo-Piñeiro J. L., Mateos P. F., Martínez-Molina E., Velázquez E. 2003; Xylanimonas cellulosilytica gen. nov., sp. nov., a xylanolytic bacterium isolated from a decayed tree ( Ulmus nigra ). Int J Syst Evol Microbiol 53:99–103 [CrossRef]
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  13. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  14. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  15. Schumann P., Weiss N., Stackebrandt E. 2001; Reclassification of Cellulomonas cellulans (Stackebrandt and Keddie 1986) as Cellulosimicrobium cellulans gen. nov., comb. nov.. Int J Syst Evol Microbiol 51:1007–1010 [CrossRef]
    [Google Scholar]
  16. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  17. Stackebrandt E., Schumann P. 2000; Description of Bogoriellaceae fam.nov., Dermacoccaceae fam. nov., Rarobacteraceae fam. nov. and Sanguibacteraceae fam. nov.and emendation of some families of the suborder Micrococcineae . Int J Syst Evol Microbiol 50:1279–1285 [CrossRef]
    [Google Scholar]
  18. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  19. Stackebrandt E., Seiler H., Schleifer K. H. 1982; Union of the genera Cellulomonas Bergey et al . and Oerskovia Prauser et al . in a redefined genus Cellulomonas . Zentbl Bakteriol Parasitenkd Infektionskr Hyg Abt 1 C3:401–409
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  21. Zimmermann O., Spröer C., Kroppenstedt R. M., Fuchs E., Köchel H. G., Funke G. 1998; Corynebacterium thomssenii sp. nov., a Corynebacterium with N -acetyl- β -glucosaminidase activity from human clinical specimens. Int J Syst Bacteriol 48:489–494 [CrossRef]
    [Google Scholar]
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