sp. nov., a medium-sized proteolytic spirochaete isolated from lesions of human periodontitis and acute necrotizing ulcerative gingivitis Free

Abstract

So far, little phenotypic heterogeneity has been detected in cultured oral treponemes with trypsin-like proteolytic activity, and all have been assigned to the species . However, comparisons of protein patterns and antigen expression in our collection of proteolytic oral treponemes occasionally identified isolates with a unique phenotype; e.g. strain OMZ 830 (=ATCC 700768), which qualified as a ‘pathogen-related oral spirochaete’ due to the presence of a ∼37 kDa protein reactive with the FlaA-specific mAb H9-2. In addition to such single isolates, a homogeneous group of seven independent strains is described that were highly motile, medium-sized, proteolytic but asaccharolytic spirochaetes and were cultured from human gingivitis, periodontitis and acute necrotizing ulcerative gingivitis in medium OMIZ-Pat supplemented with 1 % human serum and antibiotics. Growth of these spirochaetes in OMIZ-Pat was not dependent on, but was stimulated by, human or bovine serum. Carbohydrates were neither required nor stimulatory for growth. The protein and antigen patterns of total cell extracts of these organisms separated by SDS-PAGE were distinct from those of all previously cultured spirochaetes, with highest similarity to . The novel spirochaete has a 2 : 4 : 2 arrangement of the periplasmic flagella, similar to . However, the flagellin pattern as detected by immunostaining or glycan staining of Western blots readily distinguished the novel group from . Also, distinct from reference strains of , none of the novel isolates displayed sialidase or dentilisin activities, both of which are expressed by most strains of . Trypsin-like activity and other enzymes as detected by API ZYM test were similar to those of . The status of a novel species is supported by the 16S rRNA gene sequence, with 98·5 % similarity to its closest cultured relative, . The name sp. nov. is proposed (type strain OMZ 758=ATCC 700334=CIP 108088).

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2004-07-01
2024-03-29
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References

  1. Becker M. R., Paster B. J., Leys E. J., Moeschberger M. L., Kenyon S. G., Galvin J. L., Boches S. K., Dewhirst F. E., Griffen A. L. 2002; Molecular analysis of bacterial species associated with childhood caries. J Clin Microbiol 40:1001–1009 [CrossRef]
    [Google Scholar]
  2. Bickar D., Reid P. D. 1992; A high-affinity protein stain for Western blots, tissue prints, and electrophoretic gels. Anal Biochem 203:109–115 [CrossRef]
    [Google Scholar]
  3. Chan E. C. S., Siboo R., Keng T., Psarra N., Hurley R., Cheng S. L., Iugovaz I. 1993; Treponema denticola (ex Brumpt 1925 sp. nov., nom. rev and identification of new spirochete isolates from periodontal pockets. Int J Syst Bacteriol 43196–203 [CrossRef]
    [Google Scholar]
  4. Choi B.-K., Paster B. J., Dewhirst F. E., Göbel U. B. 1994; Diversity of cultivable and uncultivable oral spirochetes from a patient with severe destructive periodontitis. Infect Immun 62:1889–1895
    [Google Scholar]
  5. Dewhirst F. E., Tamer M. A., Ericson R. E., Lau C. N., Levanos V. A., Boches S. K., Galvin J. L., Paster B. J. 2000; The diversity of periodontal spirochetes by 16S rRNA analysis. Oral Microbiol Immunol 15:196–202 [CrossRef]
    [Google Scholar]
  6. Haffajee A. D., Socransky S. S. 1994; Microbial etiological agents of destructive periodontal diseases. Periodontol 2000; 5:78–111 [CrossRef]
    [Google Scholar]
  7. Lukehart S. A., Tam M. R., Hom J., Baker-Zander S. A., Holmes K. K., Nowinski R. C. 1985; Characterization of monoclonal antibodies to Treponema pallidum . J Immunol 134:585–592
    [Google Scholar]
  8. Moter A., Hoenig C., Choi B.-K., Riep B., Göbel U. B. 1998a; Molecular epidemiology of oral treponemes associated with periodontal disease. J Clin Microbiol 36:1399–1403
    [Google Scholar]
  9. Moter A., Leist G., Rudolph R., Schrank K., Choi B.-K., Wagner M., Göbel U. B. 1998b; Fluorescence in situ hybridization shows spatial distribution of as yet uncultured treponemes in biopsies from digital dermatitis lesions. Microbiology 144:2459–2467 [CrossRef]
    [Google Scholar]
  10. Page R. C. 1995; Critical issues in periodontal research. J Dent Res 74:1118–1128 [CrossRef]
    [Google Scholar]
  11. Paster B. J., Dewhirst F. E., Coleman B. C., Lau C. N., Ericson R. L. 1998; Phylogenetic analysis of cultivable oral treponemes from the Smibert collection. Int J Syst Bacteriol 48:713–722 [CrossRef]
    [Google Scholar]
  12. Paster B. J., Boches S. K., Galvin J. L., Ericson R. E., Lau C. N., Levanos V. A., Sahasrabudhe A., Dewhirst F. E. 2001; Bacterial diversity in human subgingival plaque. J Bacteriol 183:3770–3783 [CrossRef]
    [Google Scholar]
  13. Riviere G. R., Wagoner M. A., Baker-Zander S. A., Weisz K. S., Adams D. F., Simonson L., Lukehart S. A. 1991; Identification of spirochetes related to Treponema pallidum in necrotizing ulcerative gingivitis and chronic periodontitis. N Engl J Med 325:539–543 [CrossRef]
    [Google Scholar]
  14. Riviere G. R., Smith K. S., Carranza N. Jr, Tzagaoulaki E., Kay S. L., Dock M. 1995; Subgingival distribution of Treponema denticola , Treponema socranskii , and pathogen-related oral spirochetes: prevalence and relationship to periodontal status of sampled sites. J Periodontol 66:829–837 [CrossRef]
    [Google Scholar]
  15. Willis S. G., Smith K. S., Dunn V. L., Gapter L. A., Riviere K. H., Riviere G. R. 1999; Identification of seven Treponema species in health- and disease-associated dental plaque by nested PCR. J Clin Microbiol 37:687–689
    [Google Scholar]
  16. Wyss C. 1989; Dependence of proliferation of Bacteroides forsythus on exogenous N-acetylmuramic acid. Infect Immun 57:1757–1759
    [Google Scholar]
  17. Wyss C. 1992; Growth of Porphyromonas gingivalis , Treponema denticola , T. pectinovorum , T. socranskii , and T. vincentii in a chemically defined medium. J Clin Microbiol 30:2225–2229
    [Google Scholar]
  18. Wyss C. 1998; Flagellins, but not endoflagellar sheath proteins, of Treponema pallidum and of pathogen-related oral spirochetes are glycosylated. Infect Immun 66:5751–5754
    [Google Scholar]
  19. Wyss C., Choi B. K., Schüpbach P., Guggenheim B., Göbel U. B. 1996; Treponema maltophilum sp. nov., a small oral spirochete isolated from human periodontal lesions. Int J Syst Bacteriol 46:745–752 [CrossRef]
    [Google Scholar]
  20. Wyss C., Choi B. K., Schüpbach P., Guggenheim B., Göbel U. B. 1997; Treponema amylovorum sp. nov., a saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion. Int J Syst Bacteriol 47:842–845 [CrossRef]
    [Google Scholar]
  21. Wyss C., Choi B.-K., Schüpbach P., Moter A., Guggenheim B., Göbel U. B. 1999; Treponema lecithinolyticum sp. nov., a small saccharolytic spirochaete with phospholipase A and C activities associated with periodontal diseases. Int J Syst Bacteriol 49:1329–1339 [CrossRef]
    [Google Scholar]
  22. Wyss C., Dewhirst F. E., Gmür R., Thurnheer T., Xue Y., Schüpbach P., Guggenheim B., Paster B. J. 2001; Treponema parvum sp. nov., a small, glucuronic or galacturonic acid-dependent oral spirochaete from lesions of human periodontitis and acute necrotizing ulcerative gingivitis. Int J Syst Evol Microbiol 51:955–962 [CrossRef]
    [Google Scholar]
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