Proposal of gen. nov., sp. nov. and an additional genomospecies, isolated from seals, as new members of the family Free

Abstract

Phenotypic and phylogenetic studies were performed on eight Gram-negative-staining, rod-shaped bacteria isolated from seals. Biochemical and physiological studies showed identical profiles for all of the isolates and indicated that they were related to the family . 16S rRNA gene sequencing demonstrated that the organism represented a distinct cluster with two sublines within the family with <96 % sequence similarity to any recognized species. Multilocus sequence analysis (MLSA) including , and genes further confirmed these findings with the eight isolates forming a genus-like cluster with two branches. Genome relatedness as deduced from gene sequences suggested that the isolates represented a new genus with two species. On the basis of the results of the phylogenetic analysis and phenotypic criteria, it is proposed that these bacteria from seals are classified as gen. nov., sp. nov. (the type species) and genomospecies 1. The G+C content of the DNA was 39.5 mol%. The type strain of gen. nov., sp. nov. is M327/99/2 ( = CCUG 43067 = NCTC 13475 = 98-D-690B) and the reference strain of genomospecies 1 is M1765/96/5 ( = CCUG 59551 = NCTC 13474).

Funding
This study was supported by the:
  • Scottish Government Marine Directorate
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2011-12-01
2024-03-28
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