1887

Abstract

Three genera in the Rubiaceae (, and ) harbour bacterial endosymbionts within leaf nodules or galls. The present paper identifies the bacterial endophytes in three leaf-nodulating species. In order to reveal their identity and assess their phylogenetic position, 16S rRNA, and genes were sequenced from an extensive sampling of strains. This multigene approach results in a robust phylogeny, which places the bacterial endosymbionts of at two distinct positions within the genus (class ), suggesting that leaf-nodulating endosymbionts within have different origins. The endophytes of nodulated species were recognized as the closest relatives to the endosymbionts. Our results suggest that the endosymbionts of represent novel species, which can be classified as ‘ Burkholderia hispidae’, ‘ Burkholderia rigidae’ and ‘ Burkholderia schumannianae’.

Funding
This study was supported by the:
  • Institute for the Promotion of Innovation by Science and Technology (Award 71488)
  • Research Program of the Fund for Scientific Research – Flanders (Belgium) (Award G.0343.09N)
  • K.U. Leuven (Award OT/05/35)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.028019-0
2012-01-01
2024-12-02
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/1/202.html?itemId=/content/journal/ijsem/10.1099/ijs.0.028019-0&mimeType=html&fmt=ahah

References

  1. Edgar R. C. 2004; MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797 [View Article][PubMed]
    [Google Scholar]
  2. Gordon J. F. 1963; The nature and distribution within the plant of the bacteria associated with certain leaf-nodulated species of the families Myrsinaceae and Rubiaceae . PhD thesis, Imperial College, University of London, UK.
    [Google Scholar]
  3. Huelsenbeck J. P., Ronquist F. 2001; mrbayes: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755 [View Article][PubMed]
    [Google Scholar]
  4. Maddison D. R., Maddison W. P. 2002 MacClade 4: Analysis of Phylogeny and Character Evolution, Version 4.01 Sunderland, Massachusetts: Sinauer Associates;
    [Google Scholar]
  5. Miller I. M. 1990; Bacterial leaf nodule symbiosis. Adv Bot Res 17:163–234 [View Article]
    [Google Scholar]
  6. Murray R. G. E., Stackebrandt E. 1995; Taxonomic note: implementation of the provisional status Candidatus for incompletely described procaryotes. Int J Syst Bacteriol 45:186–187 [View Article][PubMed]
    [Google Scholar]
  7. Posada D., Crandall K. A. 1998; MODELTEST: testing the model of DNA substitution. Bioinformatics 14:817–818 [View Article][PubMed]
    [Google Scholar]
  8. Rambaut A., Drummond A. J. 2007; Tracer v1. http://beast.bio.ed.ac.uk/Main_Page
  9. Ronquist F., Huelsenbeck J. P. 2003; MrBayes3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574 [View Article][PubMed]
    [Google Scholar]
  10. Shimodaira H. 2002; An approximately unbiased test of phylogenetic tree selection. Syst Biol 51:492–508 [View Article][PubMed]
    [Google Scholar]
  11. Shimodaira H., Hasegawa M. 1999; Multiple comparisons of log-likelihoods with applications to phylogenetic inference. Mol Biol Evol 16:1114–1116 [CrossRef]
    [Google Scholar]
  12. Shimodaira H., Hasegawa M. 2001; CONSEL: for assessing the confidence of phylogenetic tree selection. Bioinformatics 17:1246–1247 [View Article][PubMed]
    [Google Scholar]
  13. Spilker T., Baldwin A., Bumford A., Dowson C. G., Mahenthiralingam E., LiPuma J. J. 2009; Expanded multilocus sequence typing for Burkholderia species. J Clin Microbiol 47:2607–2610 [View Article][PubMed]
    [Google Scholar]
  14. Staden R., Beal K., Bonfield J. 1998; The Staden Package. In Computer Methods in Molecular Biology vol. 132 pp. 115–130 Edited by Misener S., Krawetz S. Totowa, NJ: Humana Press;
    [Google Scholar]
  15. Stamatakis A. 2006; RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690 [View Article][PubMed]
    [Google Scholar]
  16. Swofford D. L. 2002; paup*: Phylogenetic analysis using parsimony (and other methods), version 4. Sunderland, MA: Sinauer Associates;
  17. Van Oevelen S., Prinsen E., De Wachter R., Robbrecht E. 2001; The taxonomic value of bacterial symbiont identification in African Psychotria (Rubiaceae). Syst Geogr Plants 71:557–563 [View Article]
    [Google Scholar]
  18. Van Oevelen S., De Wachter R., Vandamme P., Robbrecht E., Prinsen E. 2002; Identification of the bacterial endosymbionts in leaf galls of Psychotria (Rubiaceae, angiosperms) and proposal of ‘Candidatus Burkholderia kirkii’ sp. nov.. Int J Syst Evol Microbiol 52:2023–2027 [View Article][PubMed]
    [Google Scholar]
  19. Van Oevelen S., De Wachter R., Vandamme P., Robbrecht E., Prinsen E. 2004; Candidatus Burkholderia calva’ and ‘Candidatus Burkholderia nigropunctata’ as leaf gall endosymbionts of African Psychotria . Int J Syst Evol Microbiol 54:2237–2239 [View Article][PubMed]
    [Google Scholar]
  20. Vanlaere E., Baldwin A., Gevers D., Henry D., De Brandt E., LiPuma J. J., Mahenthiralingam E., Speert D. P., Dowson C., Vandamme P. 2009; Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp. nov. and Burkholderia lata sp. nov.. Int J Syst Evol Microbiol 59:102–111 [View Article][PubMed]
    [Google Scholar]
  21. von Faber F. C. 1912; Das erbliche Zusammenleben von Bakterien und tropischen Pflanzen. Jahrb Wiss Bot 54:285–375
    [Google Scholar]
  22. von Teichman I., Robbertse P. J., van der Merwe C. F. 1982; Contributions to the floral morphology and embryology of Pavetta gardeniifolia A. Rich. Part 2. The ovule and megasporogenesis. S Afr J Bot 1:22–27
    [Google Scholar]
  23. Werle E., Schneider C., Renner M., Völker M., Fiehn W. 1994; Convenient single-step, one tube purification of PCR products for direct sequencing. Nucleic Acids Res 22:4354–4355 [View Article][PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.028019-0
Loading
/content/journal/ijsem/10.1099/ijs.0.028019-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error