1887

Abstract

Three genera in the Rubiaceae (, and ) harbour bacterial endosymbionts within leaf nodules or galls. The present paper identifies the bacterial endophytes in three leaf-nodulating species. In order to reveal their identity and assess their phylogenetic position, 16S rRNA, and genes were sequenced from an extensive sampling of strains. This multigene approach results in a robust phylogeny, which places the bacterial endosymbionts of at two distinct positions within the genus (class ), suggesting that leaf-nodulating endosymbionts within have different origins. The endophytes of nodulated species were recognized as the closest relatives to the endosymbionts. Our results suggest that the endosymbionts of represent novel species, which can be classified as ‘ Burkholderia hispidae’, ‘ Burkholderia rigidae’ and ‘ Burkholderia schumannianae’.

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2012-01-01
2019-12-15
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