1887

Abstract

Strains Cr9-30 and Cr9-12 were isolated from alpine glacier cryoconite. Both strains were Gram-negative-staining, non-motile, rod-shaped and psychrophilic, showing good growth over the temperature range 1–20 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that the two strains formed a distinct branch within the family and were most closely related to members of the genus . The 16S rRNA gene sequence similarity between strains Cr9-30 and Cr9-12 was 99.0 %. The two strains showed highest 16S rRNA gene sequence pairwise similarity with LMG 23965 (96.6 and 96.1 % for strains Cr9-30 and Cr9-12, respectively), LMG 23964 (96.5 and 96.3 %, respectively) and LMG 21973 (96.4 and 96.2 %, respectively). The predominant cellular fatty acids were summed feature 3 (Cω7 and/or iso-C 2-OH), C and Cω7. The DNA G+C content of strain Cr9-30 was 51.0 mol%. On the basis of phenotypic characteristics and phylogenetic analysis, strains Cr9-30 and Cr9-12 represent a novel species in a new genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is Cr9-30 ( = DSM 23240 = LMG 25547).

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2011-09-01
2020-01-23
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References

  1. Cavicchioli R.. ( 2006;). Cold-adapted archaea. . Nat Rev Microbiol 4:, 331–343. [CrossRef][PubMed]
    [Google Scholar]
  2. de Boer W., Leveau J. H. J., Kowalchuk G. A., Klein Gunnewiek P. J. A., Abeln E. C. A., Figge M. J., Sjollema K., Janse J. D., van Veen J. A.. ( 2004;). Collimonas fungivorans gen. nov., sp. nov., a chitinolytic soil bacterium with the ability to grow on living fungal hyphae. . Int J Syst Evol Microbiol 54:, 857–864. [CrossRef][PubMed]
    [Google Scholar]
  3. Eder W., Wanner G., Ludwig W., Busse H.-J., Ziemke-Kägeler F., Lang E.. ( 2011;). Description of Undibacterium oligocarboniphilum sp. nov., isolated from purified water, and Undibacterium pigrum strain CCUG 49012 as the type strain of Undibacterium parvum sp. nov., and emended descriptions of the genus Undibacterium and the species Undibacterium pigrum. . Int J Syst Evol Microbiol 61:, 384–391. [CrossRef][PubMed]
    [Google Scholar]
  4. Feller G., Gerday C.. ( 2003;). Psychrophilic enzymes: hot topics in cold adaptation. . Nat Rev Microbiol 1:, 200–208. [CrossRef][PubMed]
    [Google Scholar]
  5. Felsenstein J.. ( 2009;). phylip (phylogeny inference package), version 3.69. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  6. Garrity G. M., Bell J. A., Lilburn T.. ( 2005;). Family II. Oxalobacteraceae fam. nov. In Bergey’s Manual of Systematic Bacteriology, 2nd edn, vol. 2C, p. 623. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  7. Höppener-Ogawa S., de Boer W., Leveau J. H. J., van Veen J. A., de Brandt E., Vanlaere E., Sutton H., Dare D. J., Vandamme P.. ( 2008;). Collimonas arenae sp. nov. and Collimonas pratensis sp. nov., isolated from (semi-)natural grassland soils. . Int J Syst Evol Microbiol 58:, 414–419. [CrossRef][PubMed]
    [Google Scholar]
  8. Kämpfer P., Rosselló-Mora R., Hermansson M., Persson F., Huber B., Falsen E., Busse H.-J.. ( 2007;). Undibacterium pigrum gen. nov., sp. nov., isolated from drinking water. . Int J Syst Evol Microbiol 57:, 1510–1515. [CrossRef][PubMed]
    [Google Scholar]
  9. Katayama T., Kato T., Tanaka M., Douglas T. A., Brouchkov A., Fukuda M., Tomita F., Asano K.. ( 2009;). Glaciibacter superstes gen. nov., sp. nov., a novel member of the family Microbacteriaceae isolated from a permafrost ice wedge. . Int J Syst Evol Microbiol 59:, 482–486. [CrossRef][PubMed]
    [Google Scholar]
  10. Kumar S., Tamura K., Nei M.. ( 2004;). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef][PubMed]
    [Google Scholar]
  11. Leveau J. H. J., Uroz S., de Boer W.. ( 2010;). The bacterial genus Collimonas: mycophagy, weathering and other adaptive solutions to life in oligotrophic soil environments. . Environ Microbiol 12:, 281–292. [CrossRef][PubMed]
    [Google Scholar]
  12. Loveland-Curtze J., Miteva V. I., Brenchley J. E.. ( 2009;). Herminiimonas glaciei sp. nov., a novel ultramicrobacterium from 3042 m deep Greenland glacial ice. . Int J Syst Evol Microbiol 59:, 1272–1277. [CrossRef][PubMed]
    [Google Scholar]
  13. Margesin R.. ( 2009;). Effect of temperature on growth parameters of psychrophilic bacteria and yeasts. . Extremophiles 13:, 257–262. [CrossRef][PubMed]
    [Google Scholar]
  14. Margesin R., Gander S., Zacke G., Gounot A. M., Schinner F.. ( 2003;). Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. . Extremophiles 7:, 451–458. [CrossRef][PubMed]
    [Google Scholar]
  15. Margesin R., Schinner F., Marx J. C., Gerday C.. (editors) ( 2008;). Psychrophiles: from Biodiversity to Biotechnology. Berlin/Heidelberg:: Springer;. [CrossRef]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  17. Morita R. Y.. ( 1975;). Psychrophilic bacteria. . Bacteriol Rev 39:, 144–167.[PubMed]
    [Google Scholar]
  18. Reasoner D. J., Geldreich E. E.. ( 1985;). A new medium for the enumeration and subculture of bacteria from potable water. . Appl Environ Microbiol 49:, 1–7.[PubMed]
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T.. ( 1989;). Molecular Cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  20. Sasser M.. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI.
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  22. Zhang D.-C., Schinner F., Margesin R.. ( 2010;). Pedobacter bauzanensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 60:, 2592–2595. [CrossRef][PubMed]
    [Google Scholar]
  23. Zul D., Wanner G., Overmann J.. ( 2008;). Massilia brevitalea sp. nov., a novel betaproteobacterium isolated from lysimeter soil. . Int J Syst Evol Microbiol 58:, 1245–1251. [CrossRef][PubMed]
    [Google Scholar]
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