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Abstract

Two novel strains of marine lactic acid bacteria, isolated from decaying marine algae collected from a subtropical area of Japan, are described. The isolates, designated O24-2 and O25-2, were Gram-positive, non-sporulating and non-motile. They lacked catalase and quinones. Under anaerobic cultivation conditions, lactate was produced from glucose with the production of formate, acetate and ethanol in a molar ratio of approximately 2 : 1 : 1. Under aerobic cultivation conditions, acetate and lactate were produced from carbohydrates and related compounds. The isolates were slightly halophilic, highly halotolerant and alkaliphilic. They were able to grow in 0–17.0 % (w/v) NaCl, with optimum growth of strains O24-2 and O25-2 at 1.0–3.0 and 1.0–2.0 % (w/v) NaCl, respectively. Growth of strain O24-2 was observed at pH 7.5–9.5, with optimum growth at pH 8.0–8.5. Comparative 16S rRNA gene sequence analysis revealed that the isolates occupied a phylogenetic position within the genus , showing highest similarity (99.6 %) to T129-2-1. Although sequence similarity was high, the DNA–DNA relatedness value between strain O24-2 and . T129-2-1 was 27 %, indicating that they are members of distinct species. The DNA G+C contents of O24-2 and O25-2 were 43.7 and 44.4 mol%, respectively, and DNA–DNA relatedness between the isolates was 89 %. The cell-wall peptidoglycan was type A4β, Orn--Asp. The major cellular fatty acid components were C, C and Cω9. Based on phenotypic characteristics and genetic distinctiveness, the isolates were classified as representatives of a novel species within the genus , for which the name sp. nov. is proposed; the type strain is O24-2 ( = DSM 23664 = NBRC 107172).

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2011-12-01
2020-01-22
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References

  1. Axelsson L. T.. ( 1993;). Lactic acid bacteria: classification and physiology. . In Lactic Acid Bacteria, pp. 1–63. Edited by Salminen S., von Wright A... New York:: Marcel Dekker;.
    [Google Scholar]
  2. Carlsson J., Griffith C. J.. ( 1974;). Fermentation products and bacterial yields in glucose-limited and nitrogen-limited cultures of streptococci. . Arch Oral Biol 19:, 1105–1109. [CrossRef][PubMed]
    [Google Scholar]
  3. Fukui K., Kato K., Kodama T., Ohta H., Shimamoto T., Shimono T.. ( 1988;). Kinetic study of a change in intracellular ATP level associated with aerobic catabolism of ethanol by Streptococcus mutans. . J Bacteriol 170:, 4589–4593.[PubMed]
    [Google Scholar]
  4. Gunsalus I. C., Niven C. F. Jr. ( 1942;). The effect of pH on the lactic acid fermentation. . J Biol Chem 145:, 131–136.
    [Google Scholar]
  5. Ishikawa M., Ishizaki S., Yamamoto Y., Yamasato K.. ( 2002;). Paraliobacillus ryukyuensis gen. nov., sp. nov., a new Gram-positive, slightly halophilic, extremely halotolerant, facultative anaerobe isolated from a decomposing marine alga. . J Gen Appl Microbiol 48:, 269–279. [CrossRef][PubMed]
    [Google Scholar]
  6. Ishikawa M., Nakajima K., Yanagi M., Yamamoto Y., Yamasato K.. ( 2003;). Marinilactibacillus psychrotolerans gen. nov., sp. nov., a halophilic and alkaliphilic marine lactic acid bacterium isolated from marine organisms in temperate and subtropical areas of Japan. . Int J Syst Evol Microbiol 53:, 711–720. [CrossRef][PubMed]
    [Google Scholar]
  7. Ishikawa M., Nakajima K., Itamiya Y., Furukawa S., Yamamoto Y., Yamasato K.. ( 2005;). Halolactibacillus halophilus gen. nov., sp. nov. and Halolactibacillus miurensis sp. nov., halophilic and alkaliphilic marine lactic acid bacteria constituting a phylogenetic lineage in Bacillus rRNA group 1. . Int J Syst Evol Microbiol 55:, 2427–2439. [CrossRef][PubMed]
    [Google Scholar]
  8. Ishikawa M., Tanasupawat S., Nakajima K., Kanamori H., Ishizaki S., Kodama K., Okamoto-Kainuma A., Koizumi Y., Yamamoto Y., Yamasato K.. ( 2009;). Alkalibacterium thalassium sp. nov., Alkalibacterium pelagium sp. nov., Alkalibacterium putridalgicola sp. nov. and Alkalibacterium kapii sp. nov., slightly halophilic and alkaliphilic marine lactic acid bacteria isolated from marine organisms and salted foods collected in Japan and Thailand. . Int J Syst Evol Microbiol 59:, 1215–1226. [CrossRef][PubMed]
    [Google Scholar]
  9. Jones B. E., Grant W. D., Collins N. C., Mwatha W. E.. ( 1994;). Alkaliphiles: diversity and identification. . In Bacterial Diversity and Systematics, pp. 195–230. Edited by Priest F. G., Ramos-Cormenzana A., Tindall B. J... New York:: Plenum Press;. [CrossRef]
    [Google Scholar]
  10. Kushner D. J.. ( 1978;). Life in high salt and solute concentrations: halophilic bacteria. . In Microbial Life in Extreme Environments, pp. 318–346. Edited by Kushner D. J... London:: Academic Press;.
    [Google Scholar]
  11. Kushner D. J., Kamekura M.. ( 1988;). Physiology of halophilic eubacteria. . In Halophilic Bacteria, vol. I, pp. 109–140. Edited by Rodríguez-Valera F... Boca Raton, FL:: CRC Press;.
    [Google Scholar]
  12. Liu J.-R., Tanner R. S., Schumann P., Weiss N., McKenzie C. A., Janssen P. H., Seviour E. M., Lawson P. A., Allen T. D., Seviour R. J.. ( 2002;). Emended description of the genus Trichococcus, description of Trichococcus collinsii sp. nov., and reclassification of Lactosphaera pasteurii as Trichococcus pasteurii comb. nov. and of Ruminococcus palustris as Trichococcus palustris comb. nov. in the low-G+C gram-positive bacteria. . Int J Syst Evol Microbiol 52:, 1113–1126. [CrossRef][PubMed]
    [Google Scholar]
  13. Nakajima K., Hirota K., Nodasaka Y., Yumoto I.. ( 2005;). Alkalibacterium iburiense sp. nov., an obligate alkaliphile that reduces an indigo dye. . Int J Syst Evol Microbiol 55:, 1525–1530. [CrossRef][PubMed]
    [Google Scholar]
  14. Ntougias S., Russell N. J.. ( 2001;). Alkalibacterium olivoapovliticus gen. nov., sp. nov., a new obligately alkaliphilic bacterium isolated from edible-olive wash-waters. . Int J Syst Evol Microbiol 51:, 1161–1170. [CrossRef][PubMed]
    [Google Scholar]
  15. Rhee S. K., Pack M. Y.. ( 1980;). Effect of environmental pH on fermentation balance of Lactobacillus bulgaricus. . J Bacteriol 144:, 217–221.[PubMed]
    [Google Scholar]
  16. Sakamoto M., Komagata K.. ( 1996;). Aerobic growth of and activities of NADH oxidase and NADH peroxidase in lactic acid bacteria. . J Ferment Bioeng 82:, 210–216. [CrossRef]
    [Google Scholar]
  17. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  18. Toffin L., Zink K., Kato C., Pignet P., Bidault A., Bienvenu N., Birrien J.-L., Prieur D.. ( 2005;). Marinilactibacillus piezotolerans sp. nov., a novel marine lactic acid bacterium isolated from deep sub-seafloor sediment of the Nankai Trough. . Int J Syst Evol Microbiol 55:, 345–351. [CrossRef][PubMed]
    [Google Scholar]
  19. Yamada T., Carlsson J.. ( 1975;). Regulation of lactate dehydrogenase and change of fermentation products in streptococci. . J Bacteriol 124:, 55–61.[PubMed]
    [Google Scholar]
  20. Yumoto I., Hirota K., Nodasaka Y., Yokota Y., Hoshino T., Nakajima K.. ( 2004;). Alkalibacterium psychrotolerans sp. nov., a psychrotolerant obligate alkaliphile that reduces an indigo dye. . Int J Syst Evol Microbiol 54:, 2379–2383. [CrossRef][PubMed]
    [Google Scholar]
  21. Yumoto I., Hirota K., Nodasaka Y., Tokiwa Y., Nakajima K.. ( 2008;). Alkalibacterium indicireducens sp. nov., an obligate alkaliphile that reduces indigo dye. . Int J Syst Evol Microbiol 58:, 901–905. [CrossRef][PubMed]
    [Google Scholar]
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vol. , part 12, pp. 2996 - 3002

Phase-contrast micrographs of O24-2 and O25-2.

Phylogenetic relationships between the isolates and related bacteria.

Effect of initial pH of culture medium on the product balance of glucose fermentation by the novel isolates and the type strains of related species.

Products from glucose under aerobic cultivation of strain O24-2 and the type strains of phylogenetically related species.

Cellular fatty acid composition of strain O24-2 and the type strains of phylogenetically related species.

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