1887

Abstract

Comparative 16S rDNA studies on six strains of actinomycete isolated from clinical specimens revealed that they belong to the genus and are closely related to , , , and . However, the novel organisms consistently formed a clade distinct from that of the five latter species. Determination of DNA–DNA relatedness indicated that these strains could be classified under three novel species. Based on their phenotypic and phylogenetic characters, three novel species of the genus are established: sp. nov. for IFM 0092 (=NBRC 100128=JCM 11891=DSM 44667), sp. nov. for IFM 0265 (=NBRC 100130=JCM 11893=DSM 44669) and sp. nov. for IFM 0330 (=NBRC 100131=JCM 11894=DSM 44670), IFM 0260, IFM 0636 and IFM 0833.

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2004-03-01
2020-01-17
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References

  1. Albuquerque de Barros, E. V. S., Manfio, G. P., Ribeiro Maitan, V., Mendes Bataus, L. A., Kim, S. B., Maldonado, L. A. & Goodfellow, M. ( 2003; ). Nocardia cerradoensis sp. nov., a novel isolate from Cerrado soil in Brazil. Int J Syst Evol Microbiol 53, 29–33.[CrossRef]
    [Google Scholar]
  2. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  5. Goodfellow, M. ( 1998; ). Nocardia and related genera. In Topley and Wilson's Microbiology and Microbial Infections, 9th edn, vol. 2, Systematic Bacteriology, pp. 463–489. Edited by A. Balows & B. I. Duerden. London: Arnold.
  6. Goodfellow, M., Isik, K. & Yates, E. ( 1999; ). Actinomycete systematics: an unfinished synthesis. Nova Acta Leopold 80 (312), 47–82.
    [Google Scholar]
  7. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  8. Gürtler, V., Smith, R., Mayall, B. C., Pötter-Reinemann, G., Stackebrandt, E. & Kroppenstedt, R. M. ( 2001; ). Nocardia veterana sp. nov., isolated from human bronchial lavage. Int J Syst Evol Microbiol 51, 933–936.[CrossRef]
    [Google Scholar]
  9. Hamid, M. E., Maldonado, L., Sharaf Eldin, G. S., Mohamed, M. F., Saeed, N. S. & Goodfellow, M. ( 2001; ). Nocardia africana sp. nov., a new pathogen isolated from patients with pulmonary infections. J Clin Microbiol 39, 625–630.[CrossRef]
    [Google Scholar]
  10. Kimura, M. & Ohta, T. ( 1972; ). On the stochastic model for estimation of mutational distance between homologous proteins. J Mol Evol 2, 87–90.[CrossRef]
    [Google Scholar]
  11. Kinoshita, N., Homma, Y., Igarashi, M., Ikeno, S., Hori, M. & Hamada, M. ( 2001; ). Nocardia vinacea sp. nov. Actinomycetologica 15, 1–5.[CrossRef]
    [Google Scholar]
  12. Kiska, D. L., Hicks, K. & Pettit, D. J. ( 2002; ). Identification of medically relevant Nocardia species with an abbreviated battery of tests. J Clin Microbiol 40, 1346–1351.[CrossRef]
    [Google Scholar]
  13. Lechevalier, M. P. & Lechevalier, H. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  14. Lechevalier, M. P. & Lechevalier, H. A. ( 1980; ). The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy, pp. 227–291. Edited by A. Dietz & D. W. Thayer. Arlington, VA: Society for Industrial Microbiology.
  15. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  16. Maldonado, L., Hookey, J. V., Ward, A. C. & Goodfellow, M. ( 2000; ). The Nocardia salmonicida clade, including descriptions of Nocardia cummidelens sp. nov., Nocardia fluminea sp. nov. and Nocardia soli sp. nov. Antonie van Leeuwenhoek 78, 367–377.[CrossRef]
    [Google Scholar]
  17. Mikami, Y. & Yazawa, K. ( 1989; ). Susceptibility patterns of pathogenic Nocardia to some selected antimicrobial agents and their usefulness in the identification work in a clinical laboratory. Bull JFCC 5, 89–95.
    [Google Scholar]
  18. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M. ( 1980; ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–233.[CrossRef]
    [Google Scholar]
  19. Miyadoh, M. ( 2001; ). Identification procedure at the genus level. In Identification Manual of Actinomycetes, pp. 9–19. Edited by S. Miyadoh, M. Hamada, K. Hotta, T. Kudo, A. Seino, K. Suzuki & A. Yokota. Tokyo: Business Center for Academic Societies.
  20. Orchard, V. A. & Goodfellow, M. ( 1980; ). Numerical classification of some named strains of Nocardia asteroides and related isolates from soil. J Gen Microbiol 118, 295–312.
    [Google Scholar]
  21. Poonwan, N., Kusum, M., Mikami, Y., Yazawa, K., Tanaka, Y., Gonoi, T., Hasegawa, S. & Konyama, K. ( 1995; ). Pathogenic Nocardia isolated from clinical specimens including those of AIDS patients in Thailand. Eur J Epidemiol 11, 507–512.[CrossRef]
    [Google Scholar]
  22. Roth, A., Andrees, S., Kroppenstedt, R. M., Harmsen, D. & Mauch, H. ( 2003; ). Phylogeny of the genus Nocardia based on reassessed 16S rRNA gene sequences reveals underspeciation and division of strains classified as Nocardia asteroides into three established species and two unnamed taxons. J Clin Microbiol 41, 851–856.[CrossRef]
    [Google Scholar]
  23. Saito, H. & Miura, K. ( 1983; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.
    [Google Scholar]
  24. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  25. Schaal, K. P. & Reuterberg, H. ( 1978; ). Numerical taxonomy of Nocardia asteroides. Zentbl Bakteriol Parasitol Infekt Hyg I Abt Suppl 6, 53–62.
    [Google Scholar]
  26. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  27. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  28. Suzuki, S., Yamamoto, K., Okuda, T., Nishino, M., Nakanishi, N. & Komatsubara, S. ( 2000; ). Selective isolation and distribution of Actinomadura rugatobispora strains in soil. Actinomycetologica 14, 27–33.[CrossRef]
    [Google Scholar]
  29. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  30. Thompson, J. D., Higgins, D. G. & Gibson T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  31. Wallace, R. J., Jr, Brown, B. A., Blacklock, Z. & 7 other authors ( 1995; ). New Nocardia taxon among isolates of Nocardia brasiliensis associated with invasive disease. J Clin Microbiol 33, 1528–1533.
    [Google Scholar]
  32. Wang, L., Zhang, Y., Lu, Z., Shi, Y., Liu, Z., Maldonado, L. & Goodfellow, M. ( 2001; ). Nocardia beijingensis sp. nov., a novel isolate from soil. Int J Syst Evol Microbiol 51, 1783–1788.[CrossRef]
    [Google Scholar]
  33. Yassin, A. F., Rainey, F. A., Mendrock, U., Brzezinka, H. & Schaal, K. P. ( 2000; ). Nocardia abscessus sp. nov. Int J Syst Evol Microbiol 50, 1487–1493.[CrossRef]
    [Google Scholar]
  34. Yassin, A. F., Rainey, F. A. & Steiner, U. ( 2001a; ). Nocardia cyriacigeorgici sp. nov. Int J Syst Evol Microbiol 51, 1419–1423.
    [Google Scholar]
  35. Yassin, A. F., Rainey, F. A. & Steiner, U. ( 2001b; ). Nocardia ignorata sp. nov. Int J Syst Evol Microbiol 51, 2127–2131.[CrossRef]
    [Google Scholar]
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