@article{mbs:/content/journal/ijsem/10.1099/ijs.0.02792-0, author = "Nishiguchi, Michele K. and Nair, Vinod S.", title = "Evolution of symbiosis in the Vibrionaceae: a combined approach using molecules and physiology", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2003", volume = "53", number = "6", pages = "2019-2026", doi = "https://doi.org/10.1099/ijs.0.02792-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02792-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "TBR, tree bisection and reconnection", abstract = "The family Vibrionaceae is considered to be one of the most diverse and well-studied groups of bacteria. Here, evolution is assessed within the Vibrionaceae to determine whether multiple origins of eukaryotic associations have occurred within this diverse group of bacteria. Analyses were based on a large molecular dataset, along with a matrix that consisted of 100 biochemical and restriction digest characters. By using direct optimization methods to analyse both datasets individually and in combination, a total-evidence cladogram has been produced, which supports the hypothesis that several important symbionts (both mutualistic and pathogenic) within the Vibrionaceae are not monophyletic. This leads us to consider that symbiosis (and subsequently, associations with Eukarya) has evolved multiple times within the Vibrionaceae lineage.", }