Cellulosibacter alkalithermophilus gen. nov., sp. nov., an anaerobic alkalithermophilic, cellulolytic-xylanolytic bacterium isolated from soil of a coconut garden
An obligately anaerobic, cellulolytic-xylanolytic bacterium, designated strain A6T, was isolated from soil of a coconut garden in the Bangkuntien district of Bangkok, Thailand. The strain was Gram-stain positive, catalase-negative, endospore-forming, motile and rod-shaped with a cell size of 0.2–0.3×2.0–3.0 µm. Optimal growth of strain A6T occurred at pH55 °C 9.5, 55 °C. Strain A6T fermented various carbohydrates, and the end products from the fermentation of cellobiose were acetate, ethanol, propionate and a small amount of butyrate. The major cellular fatty acids were iso-C14 : 0 3-OH, iso-C15 : 0, iso-C16 : 0 and C16 : 0. The cell-wall peptidoglycan contained meso-diaminopimelic acid. No respiratory quinones were detected. The DNA G+C content was 30.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain represented a new phyletic sublineage within the family Clostridiaceae, with <93.0 % 16S rRNA gene sequence similarity to recognized species of this family. On the basis of phenotypic, genotypic and physiological evidence, strain A6T represents a novel species of a new genus, for which the name Cellulosibacter alkalithermophilus gen. nov., sp. nov. is proposed. The type strain of the type species is A6T ( = TISTR 1915T = KCTC 5874T).
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Funding
This study was supported by the:
Royal Golden Jubilee PhD program of the Thailand Research Fund
Higher Education Research Promotion and National Research University (NRU) Project of Thailand
Office of the Higher Education Commission
Japan International Research Center for Agricultural Sciences (JIRCAS)
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