1887

Abstract

An obligately anaerobic, cellulolytic-xylanolytic bacterium, designated strain A6, was isolated from soil of a coconut garden in the Bangkuntien district of Bangkok, Thailand. The strain was Gram-stain positive, catalase-negative, endospore-forming, motile and rod-shaped with a cell size of 0.2–0.3×2.0–3.0 µm. Optimal growth of strain A6 occurred at pH 9.5, 55 °C. Strain A6 fermented various carbohydrates, and the end products from the fermentation of cellobiose were acetate, ethanol, propionate and a small amount of butyrate. The major cellular fatty acids were iso-C 3-OH, iso-C, iso-C and C. The cell-wall peptidoglycan contained -diaminopimelic acid. No respiratory quinones were detected. The DNA G+C content was 30.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain represented a new phyletic sublineage within the family , with <93.0 % 16S rRNA gene sequence similarity to recognized species of this family. On the basis of phenotypic, genotypic and physiological evidence, strain A6 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is A6 ( = TISTR 1915 = KCTC 5874).

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2012-10-01
2019-10-22
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