1887

Abstract

A 426 bp fragment of , a housekeeping gene that encodes translation initiation factor 2, was sequenced from 59 clinical isolates and type strains of species and sequences were compared. Partial sequences of 16S rRNA genes were also obtained. By comparing sequences, , , , , , and two unnamed genomospecies showed more than 85 % similarity to the type strain of the type species of the genus, . The taxonomic position of was unresolved; this species is more remotely related to . The two species and could not be clearly separated by sequence analysis. The phylogeny of the genus based on analysis was essentially congruent with relationships inferred from 16S rRNA sequence comparisons and DNA hybridization studies. Discrepancies were encountered with single strains or taxa at the periphery of the genus. Greater intraspecies variation was observed with sequences than with 16S rRNA gene sequences, with notable exceptions. The apparent subdivision of some species by 16S rRNA analysis was most likely caused by RNA operon heterogeneity.

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2004-05-01
2020-01-29
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References

  1. Berthoud, H., Frey, J. & Kuhnert, P. ( 2002; ). Characterization of Aqx and its operon: the hemolytic RTX determinant of Actinobacillus equuli. Vet Microbiol 87, 159–174.[CrossRef]
    [Google Scholar]
  2. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F. ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef]
    [Google Scholar]
  3. Christensen, H., Bisgaard, M., Angen, Ø. & Olsen, J. E. ( 2002a; ). Final classification of Bisgaard taxon 9 as Actinobacillus arthritidis sp. nov. and recognition of a novel genomospecies for equine strains of Actinobacillus lignieresii. Int J Syst Evol Microbiol 52, 1239–1246.[CrossRef]
    [Google Scholar]
  4. Christensen, H., Bisgaard, M. & Olsen, J. E. ( 2002b; ). Reclassification of equine isolates previously reported as Actinobacillus equuli, variants of A. equuli, Actinobacillus suis or Bisgaard taxon 11 and proposal of A. equuli subsp. equuli subsp. nov. and A. equuli subsp. haemolyticus subsp. nov. Int J Syst Evol Microbiol 52, 1569–1576.[CrossRef]
    [Google Scholar]
  5. Christiansen, M. ( 1917; ). En ejendommelig pyaemisk lidelse hos faar. Maanedsskr Dyrlaeg 29, 449–458 (in Danish).
    [Google Scholar]
  6. Dewhirst, F. E., Paster, B. J., Olsen, I. & Fraser, G. J. ( 1992; ). Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences. J Bacteriol 174, 2002–2013.
    [Google Scholar]
  7. Dibb, W. L., Digranes, A. & Tønjum, S. ( 1981; ). Actinobacillus lignieresii infection after a horse bite. Br Med J 283, 583–584.
    [Google Scholar]
  8. Escande, F., Grimont, F., Grimont, P. A. D. & Bercovier, H. ( 1984; ). Deoxyribonucleic acid relatedness among strains of Actinobacillus spp. and Pasteurella ureae. Int J Syst Bacteriol 34, 309–315.[CrossRef]
    [Google Scholar]
  9. Felsenstein, J. ( 1995; ). phylip (phylogenetic inference package), version 3.57c. Department of Genetics, University of Washington, Seattle, WA, USA.
  10. Fleischmann, R. D., Adams, M. D., White, O. & 37 other authors ( 1995; ). Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269, 496–512.[CrossRef]
    [Google Scholar]
  11. Friis-Møller, A., Christensen, J. J., Fussing, V., Hesselbjerg, A., Christiansen, J. & Bruun, B. ( 2001; ). Clinical significance and taxonomy of Actinobacillus hominis. J Clin Microbiol 39, 930–935.[CrossRef]
    [Google Scholar]
  12. Fussing, V., Paster, B. J., Dewhirst, F. E. & Poulsen, L. K. ( 1998; ). Differentiation of Actinobacillus pleuropneumoniae strains by sequence analysis of 16S rDNA and ribosomal intergenic regions, and development of a species specific oligonucleotide for in situ detection. Syst Appl Microbiol 21, 408–418.[CrossRef]
    [Google Scholar]
  13. Hedegaard, J., Steffensen, S. A., Nørskov-Lauritsen, N., Mortensen, K. K. & Sperling-Petersen, H. U. ( 1999; ). Identification of Enterobacteriaceae by partial sequencing of the gene encoding translation initiation factor 2. Int J Syst Bacteriol 49, 1531–1538.[CrossRef]
    [Google Scholar]
  14. Hedegaard, J., Okkels, H., Bruun, B., Kilian, M., Mortensen, K. K. & Nørskov-Lauritsen, N. ( 2001; ). Phylogeny of the genus Haemophilus as determined by comparison of partial infB sequences. Microbiology 147, 2599–2609.
    [Google Scholar]
  15. Kilian, M. ( 1976; ). A taxonomic study of the genus Haemophilus, with the proposal of a new species. J Gen Microbiol 93, 9–62.[CrossRef]
    [Google Scholar]
  16. Kilian, M., Nicolet, J. & Biberstein, E. L. ( 1978; ). Biochemical and serological characterization of Haemophilus pleuropneumoniae (Matthews and Pattison 1961) Shope 1964 and proposal of a neotype strain. Int J Syst Bacteriol 28, 20–26.[CrossRef]
    [Google Scholar]
  17. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  18. Krieg, N. R. & Garrity, G. M. ( 2001; ). On using the manual. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 15–19. Edited by D. R. Boone & R. W. Castenholz. New York: Springer.
  19. Marchandin, H., Teyssier, C., Siméon de Buochberg, M., Jean-Pierre, H., Carriere, H. & Jumas-Bilak, E. ( 2003; ). Intra-chromosomal heterogeneity between the four 16S rRNA copies in the genus Veillonella: implications for phylogeny and taxonomy. Microbiology 149, 1493–1501.[CrossRef]
    [Google Scholar]
  20. May, B. J., Zhang, Q., Li, L. L., Paustian, M. L., Whittam, T. S. & Kapur, V. ( 2001; ). Complete genomic sequence of Pasteurella multocida, Pm70. Proc Natl Acad Sci U S A 98, 3460–3465.[CrossRef]
    [Google Scholar]
  21. Mutters, R., Mannheim, W. & Bisgaard, M. ( 1989; ). Taxonomy of the group. In Pasteurella and Pasteurellosis, pp 3–34. Edited by C. Adlam & J. M. Rutter. London: Academic Press.
  22. Olsen, I. & Møller, K. ( 2004; ). Genus Actinobacillus Brumpt 1910, 849AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer (in press).
  23. Olsen, I., Dewhirst, F. E., Paster, B. J. & Busse, H.-J. ( 2004; ). Family Pasteurellaceae Pohl 1981, 382VP. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer (in press).
  24. Palys, T., Nakamura, L. K. & Cohan, F. M. ( 1997; ). Discovery and classification of ecological diversity in the bacterial world: the role of DNA sequence data. Int J Syst Bacteriol 47, 1145–1156.[CrossRef]
    [Google Scholar]
  25. Phillips, J. E. ( 1984; ). Genus III. Actinobacillus Brumpt 1910, 849AL. In Bergey's Manual of Systematic Bacteriology, pp. 570–575. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  26. Pohl, S., Bertschinger, H. U., Frederiksen, W. & Mannheim, W. ( 1983; ). Transfer of Haemophilus pleuropneumoniae and the Pasteurella haemolytica-like organism causing porcine necrotic pleuropneumonia to the genus Actinobacillus (Actinobacillus pleuropneumoniae comb. nov.) on the basis of phenotypic and deoxyribonucleic acid relatedness. Int J Syst Bacteriol 33, 510–514.[CrossRef]
    [Google Scholar]
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
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vol. , part 3, pp. 635 - 644

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