1887

Abstract

Five strains (Mizu2-1, Gon2-7, Koba6-1, Koyu2-2 and Miya2-2) of lactic acid bacteria (LAB) were isolated from flowers in Oze National Park, Japan, using anaerobic cultivation. The five isolates were found to share identical 16S rRNA gene sequences. The isolates exhibited low levels of 16S rRNA gene sequence similarity to known LAB; the closest recognized relatives of strain Mizu2-1 were the type strains of (94.9 %), (94.1 %) and (93.9 %). Comparative analyses of and gene sequences demonstrated that the novel isolates did not show significant relationships to other species. The strains were Gram-stain-positive, catalase-negative and heterofermentative. Anaerobic growth was better than aerobic growth. The isolates utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose and fructose. On the basis of phenotypic characteristics and phylogenetic data, the isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Mizu2-1 ( = JCM 17196  = DSM 23829).

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2011-10-01
2020-01-23
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Supplements

vol. , part 10, pp. 2435 - 2438

Flowers collected from Oze National Park, from which strains of sp. nov. were isolated.

Phylogenetic trees showing relationships based on 16S rRNA gene sequences between strains of sp. nov. and related species, reconstructed using the maximum-parsimony (Fig. S2) and maximum-likelihood (Fig. S3) methods.

Phylogenetic relationships of strain Mizu2-1 ( sp. nov.) to related species based on (Fig. S4) and (Fig. S5) gene sequences, obtained by the neighbour-joining method.

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