1887

Abstract

The taxonomic positions of and were examined using phenotypic tests, 16S rRNA gene sequencing and DNA–DNA hybridization. The two species displayed very similar sugar-fermentation patterns and produced the -form of lactic acid. Phylogenetic analysis showed that is the closest neighbour of , sharing about 99·7 % similarity in the 16S rRNA gene sequence. DNA–DNA hybridization experiments further reinforced these results, demonstrating a 98·2 % reassociation level between LMG 17699 and DSM 14295. On the other hand, strain RO5, an isolate from paddy rice silage, exhibited 99·4 % 16S rRNA gene sequence similarity and a DNA reassociation level of more than 97 % with both type strains. Therefore, it is proposed that the species should be reclassified as .

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2004-03-01
2020-01-24
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vol. , part 2, pp. 463–465

A phylogenetic tree constructed using a larger data set than shown in Fig. 1, details of acid production from carbohydrates (Table I) and DNA–DNA reassociation data (Table II) are available to download. [PDF]



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