1887

Abstract

The taxonomic positions of and were examined using phenotypic tests, 16S rRNA gene sequencing and DNA–DNA hybridization. The two species displayed very similar sugar-fermentation patterns and produced the -form of lactic acid. Phylogenetic analysis showed that is the closest neighbour of , sharing about 99·7 % similarity in the 16S rRNA gene sequence. DNA–DNA hybridization experiments further reinforced these results, demonstrating a 98·2 % reassociation level between LMG 17699 and DSM 14295. On the other hand, strain RO5, an isolate from paddy rice silage, exhibited 99·4 % 16S rRNA gene sequence similarity and a DNA reassociation level of more than 97 % with both type strains. Therefore, it is proposed that the species should be reclassified as .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02783-0
2004-03-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/2/ijs540463.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02783-0&mimeType=html&fmt=ahah

References

  1. Björkroth K. J., Schillinger U., Geisen R., Weiss N., Hoste B., Holzapfel W. H., Korkeala H. J., Vandamme P. 2002; Taxonomic study of Weissella confusa and description of Weissella cibaria sp. nov., detected in food and clinical samples. Int J Syst Evol Microbiol 52:141–148
    [Google Scholar]
  2. Cai Y., Ohmomo S., Kumai S. 1994; Distribution and lactate fermentation characteristics of lactic acid bacteria on forage crops and grasses. J Jpn Soc Grassl Sci 39:420–428
    [Google Scholar]
  3. Cai Y., Benno Y., Ogawa M., Ohmomo S., Kumai S., Nakase T. 1998; Influence of Lactobacillus spp. from an inoculant and of Weissella and Leuconostoc spp. from forage crops on silage fermentation. Appl Environ Microbiol 64:2982–2987
    [Google Scholar]
  4. Choi H.-J., Cheigh C.-I., Kim S.-B., Lee J.-C., Lee D.-W., Choi S.-W., Park J.-M., Pyun Y.-R. 2002; Weissella kimchii sp. nov., a novel lactic acid bacterium from kimchi. Int J Syst Evol Microbiol 52:507–511
    [Google Scholar]
  5. Collins M. D., Samelis J., Metaxopoulos J., Wallbanks S. 1993; Taxonomic studies on some leuconostoc-like organisms from fermented sausages: description of a new genus Weissella for the Leuconostoc paramesenteroides group of species. J Appl Bacteriol 75:595–603 [CrossRef]
    [Google Scholar]
  6. Ennahar S., Cai Y., Fujita Y. 2003; Phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16S ribosomal DNA analysis. Appl Environ Microbiol 69:444–451 [CrossRef]
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  8. Lee J.-S., Lee K. C., Ahn J.-S., Mheen T.-I., Pyun Y.-R., Park Y.-H. 2002; Weissella koreensis sp. nov., isolated from kimchi. Int J Syst Evol Microbiol 52:1257–1261 [CrossRef]
    [Google Scholar]
  9. Magnusson J., Jonsson H., Schnürer J., Roos S. 2002; Weissella soli sp. nov., a lactic acid bacterium isolated from soil. Int J Syst Evol Microbiol 52:831–834 [CrossRef]
    [Google Scholar]
  10. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Satomi M., Kimura B., Mizoi M., Sato T., Fujii T. 1997; Tetragenococcus muriaticus sp. nov., a new moderately halophilic lactic acid bacterium isolated from fermented squid liver sauce. Int J Syst Bacteriol 47:832–836 [CrossRef]
    [Google Scholar]
  12. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 124:11–16
    [Google Scholar]
  13. Tanasupawat S., Shida O., Okada S., Komagata K. 2000; Lactobacillus acidipiscis sp. nov. and Weissella thailandensis sp. nov., isolated from fermented fish in Thailand. Int J Syst Evol Microbiol 50:1479–1485 [CrossRef]
    [Google Scholar]
  14. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02783-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02783-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error