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Abstract

A Gram-staining-negative, motile, agarolytic bacterium, designated M-M1, was isolated from marine sand obtained from Geoje Island, South Sea, Korea, and its taxonomic position was investigated using a polyphasic taxonomic approach. Strain M-M1 grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. It did not grow in the presence of >7 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M-M1 fell within the clade comprising members of the genus , clustering with TMA1, CBMAI 722 and JC2041, with which it exhibited 16S rRNA gene sequence similarity values of 96.4, 96.0 and 94.9 % respectively. Strain M-M1 exhibited 94.7–95.2 % 16S rRNA gene sequence similarity to the other species of the genus . Strain M-M1 contained Q-8 as the predominant ubiquinone and Cω7 and/or iso-C 2-OH, C and Cω7 as the major fatty acids. The DNA G+C content was 44.2 mol%. Strain M-M1 could be differentiated from phylogenetically related species of the genus by differences in some phenotypic properties. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain M-M1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M-M1 ( = KCTC 23343  = CCUG 60020).

Funding
This study was supported by the:
  • , 21C Frontier Program of Microbial Genomics and Applications , (Award 11-2008-00-002-00)
  • , Ministry of Education, Science and Technology (MEST) of the Republic of Korea
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2011-11-01
2020-09-29
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