As part of an undergraduate microbiology course, a yellow–orange pigmented, Gram-staining negative, rod-shaped, non-motile bacterial strain, designated CTMT, was isolated from a creek in North-central Pennsylvania during the winter of 2006. The 16S rRNA gene sequence of the strain showed ~97 % similarity to that of Chryseobacterium soldanellicola PSD1-4T and Chryseobacterium soli JS6-6T, while the protein-coding gyrB gene sequence of strain CTMT showed <87 % similarity to those of its two closest relatives. Using a polyphasic approach, strain CTMT was characterized and compared to these and other closely related species of the genus Chryseobacterium. Strain CTMT was similar to other strains of the genus Chryseobacterium in that it contained MK-6 as its major respiratory quinone, produced flexirubin-type pigments, oxidase and catalase, hydrolysed DNA, gelatin and aesculin and contained the fatty acids iso-C15 : 0, iso-C17 : 1ω9c, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω6c, C16 : 1ω7c and/or iso-C15 : 0 2-OH). Based on the results of this study, strain CTMT represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium piperi sp. nov. is proposed. The type strain is CTMT ( = ATCC BAA-1782T = CCUG 57707T = JCM 15960T = DSM 22249T = KCTC 23267T).
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