sp. nov., a moderate halophile isolated from a marine solar saltern in Korea, with emended description of the genus Free

Abstract

A Gram-positive, motile, round to ellipsoidal, endospore-forming, rod-shaped bacterial strain, SF-57, was isolated from a marine solar saltern in Korea. This organism grew between 4 and 39 °C, with optimum growth at 30 °C. Strain SF-57 grew in the presence of 0·5–15·0 % NaCl, with optimum growth at 2–3 % NaCl. The peptidoglycan type of strain SF-57 was A1 linked directly through -Lys. In strain SF-57, menaquinone-7 (MK-7) was the predominant isoprenoid quinone and anteiso-C was the major fatty acid. The DNA G+C content was 41·8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SF-57 formed a coherent cluster with , with a bootstrap resampling value of 100 %. The level of 16S rRNA gene sequence similarity between strain SF-57 and DSM 1297 was 98·9 %. The mean DNA–DNA relatedness level between strain SF-57 and the type strain of was 20·6 %. Based on phenotypic properties, phylogenetic analyses and genomic data, strain SF-57 merits placement in the genus as a representative of a novel species, for which the name sp. nov. is proposed. The type strain is SF-57 (=KCCM 41644=JCM 11810).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02779-0
2004-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/4/ijs541317.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02779-0&mimeType=html&fmt=ahah

References

  1. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett Appl Microbiol 13:202–206
    [Google Scholar]
  2. Baumann L., Baumann P. 1981 The marine Gram-negative eubacteria; genera Photobacterium ,Beneckea , Alteromonas , Pseudomonas , and Alcaligenes . In The Prokaryotes pp  1302–1330 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [CrossRef]
    [Google Scholar]
  4. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  6. Farrow J. A. E., Wallbanks S., Collins M. D. 1994; Phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-spore-forming genera Caryophanon , Exiguobacterium , Kurthia , and Planococcus . Int J Syst Bacteriol 44:74–82 [CrossRef]
    [Google Scholar]
  7. Komagata K., Suzuki K. I. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–206
    [Google Scholar]
  8. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  9. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  10. Levring T. 1946; Some culture experiments with Ulva and artificial seawater. K Fysiogr Sallsk Lund Forh 16:45–56
    [Google Scholar]
  11. MacKenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N -heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160
    [Google Scholar]
  12. Rheims H., Frühling A., Schumann P., Rohde M., Stackebrandt E. 1999; Bacillus silvestris sp. nov., a new member of the genus Bacillus that contains lysine in its cell wall. Int J Syst Bacteriol 49:795–802 [CrossRef]
    [Google Scholar]
  13. Rüger H.-J. 1983; Differentiation of Bacillus globisporus , Bacillus marinus comb. nov., Bacillus aminovorans , and Bacillus insolitus . Int J Syst Bacteriol 33:157–161 [CrossRef]
    [Google Scholar]
  14. Rüger H. J., Richter G. 1979; Bacillus globisporus subsp. marinus subsp. nov. Int J Syst Bacteriol 29:196–203 [CrossRef]
    [Google Scholar]
  15. Rüger H. J., Fritze D., Spröer C. 2000; New psychrophilic and psychrotolerant Bacillus marinus strains from tropical and polar deep-sea sediments and emended description of the species. Int J Syst Evol Microbiol 50:1305–1313 [CrossRef]
    [Google Scholar]
  16. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101 Newark, DE: MIDI;
    [Google Scholar]
  17. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  18. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  19. Stackebrandt E., Ludwig W., Weizenegger M., Dorn S., McGill T. J., Fox G. E., Woese C. E., Schubert W., Schleifer K.-H. 1987; Comparative 16S rRNA oligonucleotide analyses and murein types of round-spore-forming bacilli and non-spore-forming relatives. J Gen Microbiol 133:2523–2529
    [Google Scholar]
  20. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  23. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  24. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Lee K.-C., Weiss N., Kho Y. H., Kang K. H., Park Y.-H. 2001a; Sporosarcina aquimarina sp. nov., a bacterium isolated from seawater in Korea, and transfer of Bacillus globisporus (Larkin and Stokes 1967), Bacillus psychrophilus (Nakamura 1984) and Bacillus pasteurii (Chester 1898) to the genus Sporosarcina as Sporosarcina globispora comb.nov., Sporosarcina psychrophila comb. nov. and Sporosarcina pasteurii comb. nov., and emended description of the genus Sporosarcina . Int J Syst Evol Microbiol 51:1079–1086 [CrossRef]
    [Google Scholar]
  26. Yoon J.-H., Weiss N., Lee K.-C., Lee I.-S., Kang K. H., Park Y.-H. 2001b; Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with l-lysine in the cell wall, and reclassification of Bacillus marinus Rüger 1983 as Marinibacillus marinus gen. nov., comb. nov. Int J Syst Evol Microbiol 51, 2087–2093 [CrossRef]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02779-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02779-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed