1887

Abstract

A novel Gram-positive, strictly aerobic and non-motile bacterial strain, S6-4, was isolated from a Korean alcohol fermentation starter. Optimal growth occurred at 37 °C, at pH 8 and in 1 % (w/v) NaCl. The isolate was positive for oxidase and catalase. It assimilated various sugars and acids were produced from several carbohydrates. The major cell-wall sugars were galactose and arabinose. The major fatty acids of strain S6-4 were C, Cω9, Cω9 and 10-methyl C (tuberculostearic acid). The predominant isoprenoid quinone was menaquinone MK-9(H) and peptidoglycan amino acids were -diaminopimelic acid, alanine, glycine and glutamic acid. The strain contained mycolic acids. According to phylogenetic analysis based on 16S rRNA gene sequences, strain S6-4 was most closely related to DSM 20132 (98.1 % similarity). The genomic DNA G+C content of strain S6-4 was 73.6 mol% and DNA–DNA hybridization values with related strains were below 33±4 %. On the basis of phenotypic, genotypic and phylogenetic data, strain S6-4 represents a novel species in the genus , for which the name sp. nov. is proposed; the type strain is S6-4 ( = KACC 15032  = JCM 17162).

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2011-10-01
2020-01-23
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vol. , part 10, pp. 2430 - 2434

Fluorescence images of DNA–DNA hybridization through the genome-spotted microarrays. DNA–DNA hybridization values obtained with 1 µg of Cy5-dUTP labelled target DNA from (a) strain S6-4 , (b) DSM 20132 and (c) IFO 14764



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