1887

Abstract

Two Gram-negative, aerobic strains, Y215 and Y226, were isolated from sediment from Yueqing Bay, Zhejiang Province, China. The two novel strains were both positive for oxidase activity, nitrate reduction, and aesculin and casein decomposition, but negative for gelatin and tyrosine decomposition. Catalase activity, and starch and Tween 80 decomposition differed between the two strains. Cells of both novel strains were rod-shaped in young cultures and ovoid in older cultures. Optimum NaCl concentration and pH range for growth of both strains were 2.0–3.0 % (w/v) and 7.0–8.0, respectively, whereas the optimum growth temperature for strain Y215 (25–30 °C) was lower than that for strain Y226 (30–37 °C). The genomic DNA G+C contents of strains Y215 and Y226 were 54.0 and 56.7 mol%, respectively. The major fatty acids in both isolates were iso-C and iso-Cω9, which was also the case in the reference strains apart from , which possessed Cω7 as the predominant fatty acid. The predominant isoprenoid quinone was Q-8 and the major polar lipids of both strains were phosphatidylethanolamine, phosphatidylglycerol and an unknown glycolipid. Both strains had highest 16S rRNA gene sequence similarity to members of the genus . Strain Y215 was closely related to the type strains of (97.6 %) and (97.5 %), whereas strain Y226 was closely related to the type strain of (97.6 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strains Y215 and Y226 fell into two separate clusters. The DNA–DNA relatedness values of strain Y215 with TF-17 and CN85 were 34.1 and 32.8 %, respectively, whereas that between strain Y226 and SM-1 was 38.0 %; these values are significantly lower than the threshold value for the delineation of bacterial species. On the basis of their distinct taxonomic characteristics, the two isolates represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed; the type strains are Y215 ( = CGMCC 1.10657 = JCM 17211) and Y226 ( = CGMCC 1.10658 = JCM 17212), respectively.

Funding
This study was supported by the:
  • Chinese Offshore Investigation and Assessment (Award 908-ZC-I-02)
  • Scientific Research Fund of the Second Institute of Oceanography (Award JG1024)
  • National Natural Science Foundation of China (Award 30970002)
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2012-03-01
2024-12-12
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References

  1. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  2. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  3. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  4. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  5. González J. M., Mayer F., Moran M. A., Hodson R. E., Whitman W. B. 1997; Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47:369–376 [View Article][PubMed]
    [Google Scholar]
  6. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  7. Kates M. 1986 Techniques of Lipidology, 2nd edn. Amsterdam: Elsevier;
    [Google Scholar]
  8. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  9. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  10. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184[PubMed]
    [Google Scholar]
  11. Mata J. A., Martínez-Cánovas J., Quesada E., Béjar V. 2002; A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25:360–375 [View Article][PubMed]
    [Google Scholar]
  12. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr A 479:297–306 [View Article][PubMed]
    [Google Scholar]
  13. Mikhailov V. V., Romanenko L. A., Ivanova E. P. 2006; The genus Alteromonas and related proteobacteria. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn. vol. 6 pp. 597–645 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  14. Miyazaki M., Nogi Y., Ohta Y., Hatada Y., Fujiwara Y., Ito S., Horikoshi K. 2008; Microbulbifer agarilyticus sp. nov. and Microbulbifer thermotolerans sp. nov., agar-degrading bacteria isolated from deep-sea sediment. Int J Syst Evol Microbiol 58:1128–1133 [View Article][PubMed]
    [Google Scholar]
  15. Nishijima M., Takadera T., Imamura N., Kasai H., An K.-D., Adachi K., Nagao T., Sano H., Yamasato K. 2009; Microbulbifer variabilis sp. nov. and Microbulbifer epialgicus sp. nov., isolated from Pacific marine algae, possess a rod–coccus cell cycle in association with the growth phase. Int J Syst Evol Microbiol 59:1696–1707 [View Article][PubMed]
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  17. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  18. Tang S.-K., Wang Y., Cai M., Lou K., Mao P.-H., Jin X., Jiang C.-L., Xu L.-H., Li W.-J. 2008; Microbulbifer halophilus sp. nov., a moderately halophilic bacterium from north-west China. Int J Syst Evol Microbiol 58:2036–2040 [View Article][PubMed]
    [Google Scholar]
  19. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  20. Wang C.-S., Wang Y., Xu X.-W., Zhang D.-S., Wu Y.-H., Wu M. 2009; Microbulbifer donghaiensis sp. nov., isolated from marine sediment of the East China Sea. Int J Syst Evol Microbiol 59:545–549 [View Article][PubMed]
    [Google Scholar]
  21. Wayne L. G., Brenner J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  22. Xu X.-W., Wu Y.-H., Zhou Z., Wang C.-S., Zhou Y.-G., Zhang H.-B., Wang Y., Wu M. 2007; Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. Int J Syst Evol Microbiol 57:1619–1624 [View Article][PubMed]
    [Google Scholar]
  23. Xu X.-W., Wu Y.-H., Wang C.-S., Yang J.-Y., Oren A., Wu M. 2008; Marinobacter pelagius sp. nov., a moderately halophilic bacterium. Int J Syst Evol Microbiol 58:637–640 [View Article][PubMed]
    [Google Scholar]
  24. Yoon J. H., Kim I. G., Shin D. Y., Kang K. H., Park Y. H. 2003a; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [View Article][PubMed]
    [Google Scholar]
  25. Yoon J. H., Kim H., Kang K. H., Oh T. K., Park Y. H. 2003b; Transfer of Pseudomonas elongata Humm 1946 to the genus Microbulbifer as Microbulbifer elongatus comb. nov.. Int J Syst Evol Microbiol 53:1357–1361 [View Article][PubMed]
    [Google Scholar]
  26. Yoon J. H., Kim I. G., Oh T. K., Park Y. H. 2004; Microbulbifer maritimus sp. nov., isolated from an intertidal sediment from the Yellow Sea, Korea. Int J Syst Evol Microbiol 54:1111–1116 [View Article][PubMed]
    [Google Scholar]
  27. Yoon J. H., Jung S. Y., Kang S. J., Oh T. K. 2007; Microbulbifer celer sp. nov., isolated from a marine solar saltern of the Yellow Sea in Korea. Int J Syst Evol Microbiol 57:2365–2369 [View Article][PubMed]
    [Google Scholar]
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