Two Gram-negative, rod-shaped, non-spore-forming strains, designated 08RB2639T and 08RB2781-1, were isolated from a sheep (Ovis aries) and a domestic boar (Sus scrofa domestica), respectively. By 16S rRNA gene sequencing, the isolates revealed identical sequences and were shown to belong to the Alphaproteobacteria. They exhibited 97.8 % 16S rRNA gene sequence similarity with Ochrobactrum rhizosphaerae PR17T, O. pituitosum CCUG 50899T, O. tritici SCII24T and O. haematophilum CCUG 38531T and 97.4 % sequence similarity with O. cytisi ESC1T, O. anthropi LMG 3331T and O. lupini LUP21T. The recA gene sequences of the two isolates showed only minor differences (99.5 % recA sequence similarity), and strain 08RB2639T exhibited the highest recA sequence similarity with Ochrobactrum intermedium CCUG 24694T (91.3 %). The quinone system was ubiquinone Q-10, with minor amounts of Q-9 and Q-11, the major polyamines were spermidine, putrescine and sym-homospermidine and the major lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine, with moderate amounts of the Ochrobactrum-specific unidentified aminolipid AL2. The major fatty acids (>20 %) were C18 : 1ω7c and C19 : 0 cyclo ω8c. These traits were in excellent agreement with the assignment of the isolates to the genus Ochrobactrum. DNA–DNA relatedness and physiological and biochemical tests allowed genotypic and phenotypic differentiation from other members of the genus Ochrobactrum. Hence, it is concluded that the isolates represent a novel species, for which the name Ochrobactrum pecoris sp. nov. is proposed (type strain 08RB2639T = DSM 23868T = CCUG 60088T = CCM 7822T).
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
(editors) 1994Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
HolmesB.,
PopoffM.,
KiredjianM.,
KerstersK.1988; Ochrobactrum anthropi gen. nov., sp. nov. from human clinical specimens and previously known as Group Vd. Int J Syst Bacteriol 38:406–416 [View Article]
KämpferP.,
KroppenstedtR. M.1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [View Article]
KämpferP.,
SteiofM.,
DottW.1991; Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [View Article]
KämpferP.,
BuczolitsS.,
AlbrechtA.,
BusseH.-J.,
StackebrandtE.2003; Towards a standardized format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov.. Int J Syst Evol Microbiol 53:893–896 [View Article][PubMed]
LechnerU.,
BaumbachR.,
BeckerD.,
KitunenV.,
AulingG.,
Salkinoja-SalonenM.1995; Degradation of 4-chloro-2-methylphenol by an activated sludge isolate and its taxonomic description. Biodegradation 6:83–92 [View Article][PubMed]
TeyssierC.,
MarchandinH.,
Jean-PierreH.,
MasnouA.,
DusartG.,
Jumas-BilakE.2007; Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae, isolated from human clinical samples. Int J Syst Evol Microbiol 57:1007–1013 [View Article][PubMed]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
TindallB. J.1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130[CrossRef]
TripathiA. K.,
VermaS. C.,
ChowdhuryS. P.,
LebuhnM.,
GattingerA.,
SchloterM.2006; Ochrobactrum oryzae sp. nov., an endophytic bacterial species isolated from deep-water rice in India. Int J Syst Evol Microbiol 56:1677–1680 [View Article][PubMed]
TrujilloM. E.,
WillemsA.,
AbrilA.,
PlanchueloA. M.,
RivasR.,
LudeñaD.,
MateosP. F.,
Martínez-MolinaE.,
VelázquezE.2005; Nodulation of Lupinus albus by strains of Ochrobactrum lupini sp. nov.. Appl Environ Microbiol 71:1318–1327 [View Article][PubMed]
VelascoJ.,
RomeroC.,
López-GoñiI.,
LeivaJ.,
DíazR.,
MoriyónI.1998; Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella spp.. Int J Syst Bacteriol 48:759–768 [View Article][PubMed]
YokotaA.,
Akagawa-MatsushitaM.,
HiraishiA.,
KatayamaY.,
UrakamiT.,
YamasatoK.1992; Distribution of quinone systems in microorganisms: Gram-negative eubacteria. Bull Jpn Fed Cult Coll 8:136–171
ZiemkeF.,
HöfleM. G.,
LalucatJ.,
Rosselló-MoraR.1998; Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov.. Int J Syst Bacteriol 48:179–186 [View Article][PubMed]