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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 61, Issue 9

sp. nov., isolated from farm animals Free

Abstract

Two Gram-negative, rod-shaped, non-spore-forming strains, designated 08RB2639 and 08RB2781-1, were isolated from a sheep () and a domestic boar (), respectively. By 16S rRNA gene sequencing, the isolates revealed identical sequences and were shown to belong to the . They exhibited 97.8 % 16S rRNA gene sequence similarity with PR17, CCUG 50899, SCII24 and CCUG 38531 and 97.4 % sequence similarity with ESC1, LMG 3331 and LUP21. The gene sequences of the two isolates showed only minor differences (99.5 % sequence similarity), and strain 08RB2639 exhibited the highest sequence similarity with CCUG 24694 (91.3 %). The quinone system was ubiquinone Q-10, with minor amounts of Q-9 and Q-11, the major polyamines were spermidine, putrescine and -homospermidine and the major lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine, with moderate amounts of the -specific unidentified aminolipid AL2. The major fatty acids (>20 %) were Cω7 and C cyclo ω8. These traits were in excellent agreement with the assignment of the isolates to the genus . DNA–DNA relatedness and physiological and biochemical tests allowed genotypic and phenotypic differentiation from other members of the genus . Hence, it is concluded that the isolates represent a novel species, for which the name sp. nov. is proposed (type strain 08RB2639  = DSM 23868  = CCUG 60088  = CCM 7822).

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2011-09-01
2025-03-25
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Ochrobactrum pecoris sp. nov., isolated from farm animals
Int J Syst Evol Microbiol 61, 2278 (2011); https://doi.org/10.1099/ijs.0.027631-0
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