1887

International Journal of Systematic and Evolutionary Microbiology

Volume 54, Issue 4

Identification of the bacterial endosymbionts of the marine ciliate (Ciliophora, Hypotrichia) and proposal of ‘ Devosia euplotis’ Free

Abstract

This paper reports the identification of bacterial endosymbionts that inhabit the cytoplasm of the marine ciliated protozoon . Ultrastructural and full-cycle rRNA approaches were used to reveal the identity of these bacteria. Based on analysis of 16S rRNA gene sequences, evolutionary trees were constructed; these placed the endosymbiont in the genus in the -. The validity of this finding was also shown by fluorescence hybridization with a -specific oligonucleotide probe. Differences at the 16S rRNA gene level (which allowed the construction of a species-specific oligonucleotide probe) and the peculiar habitat indicate that the endosymbiont represents a novel species. As its cultivation has not been successful to date, the provisional name ‘ Devosia euplotis' is proposed. The species- and group-specific probes designed in this study could represent convenient tools for the detection of ‘ Devosia euplotis' and -like bacteria in the environment.

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Keyword(s): FISH, fluorescence in situ hybridization
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2004-07-01
2019-10-16
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Identification of the bacterial endosymbionts of the marine ciliate Euplotes magnicirratus (Ciliophora, Hypotrichia) and proposal of ‘Candidatus Devosia euplotis’
Int J Syst Evol Microbiol 54, 1151 (2004); https://doi.org/10.1099/ijs.0.02759-0
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vol. , part 4, pp. 1151 – 1156

Similarity matrix for 16S rRNA gene sequences of ' Devosia euplotis' and related organisms.

FISH, demonstrating the identity of ' Devosia euplotis' by using species-specific bacterial probe DevEup_993.

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