1887

Abstract

The taxonomy of heterocystous cyanobacteria belonging to the genera and has long been a matter of debate, but their phylogenetic relationships are still not well understood. Our aim was to compare the phylogeny and morphology of members of these genera, which exhibit basal–apical polarity. A phylogeny was reconstructed on the basis of 16S rRNA gene sequences and compared with the morphological characterization of new isolates and environmental samples. Strains isolated from several rivers and streams showed a high degree of tapering when they were cultured in a nutrient-rich medium. However, clear differences were apparent when they were transferred to a nutrient-poor medium. Some strains showed a low degree of tapering and other morphological features corresponding to the genus , such as false branching, whereas others maintained the morphological characteristics of the genus . Phylogenetic analysis was congruent with the phenotypic characterization, in which the strains and environmental samples of the and morphotypes could be clearly separated. Isolates with a low degree of tapering and natural samples of were grouped in the same cluster, but strains of the genus fell into well separated clades. Results from this study showed that representatives of the genus share most morphological and developmental properties and a high degree of 16S rRNA gene sequence similarity. However, although similar and sometimes overlapping morphologies may occur in isolates of the genus , these morphotypes may be distinguished on the basis of their clear genetic divergence.

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2011-12-01
2020-01-21
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vol. , part 12, pp. 3039 - 3051

Neighbour-joining tree based on analysis of 16S rRNA gene of representatives of the families Microchaetaceae and Rivulariaceae (Subsection IV.II of the current bacteriological approach) showing the position of the sequences obtained in the present study (in bold). The family Scytonemataceae (Subsection IV.I) was used as outgroup. Numbers at nodes indicate bootstrap values ≥50%. Bar, 0.01 substitutions per nucleotide position. . Maximum-parsimony tree based on analysis of 16S rRNA gene of representatives of the families Microchaetaceae and Rivulariaceae (Subsection IV.II), showing the position of the sequences obtained in the present study (in bold). The family Scytonemataceae (Subsection IV.I) was used as outgroup . Numbers at nodes indicate bootstrap values ≥50%.

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