1887

Abstract

An arsenic- and radiation-resistant bacterium, strain Wt/1a, was isolated from water from an arsenic-contaminated aquifer located in the Chakdah district of West Bengal, India. The bacterium stains Gram-negative and is rod-shaped, non-motile, non-sporulating and red-pigmented. Cell-wall peptidoglycan contains ornithine as the diamino acid, MK-8 is the major menaquinone, C and C are the major fatty acids and the DNA G+C content of the organism is 65·8 mol%. Based on these phenotypic and chemotaxonomic characteristics, strain Wt/1a was identified as a member of the genus . Strain Wt/1a exhibited maximum 16S rRNA gene sequence similarity (95 %) with ; however, strain Wt/1a exhibited only 14 % similarity to IAM 13005 at the DNA–DNA level. Furthermore, strain Wt/1a (compared to IAM 13005) is more resistant to arsenate and arsenite, is positive for arginine dihydrolase, utilizes a number of carbon sources and exhibits quantitative differences in fatty acid composition and qualitative differences in lipid composition. Strain Wt/1a is identified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is Wt/1a (=MTCC 4913=DSM 15307).

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2004-03-01
2019-09-16
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References

  1. Bansal, N., Sinha, I. & Virdi, J. S. ( 2000; ). Arsenic and cadmium resistance in environmental isolates of Yersinia enterocolitica and Yersinia intermedia. Can J Microbiol 46, 481–484.[CrossRef]
    [Google Scholar]
  2. Bhattacharya, P., Chatterjee, D. & Jacks, G. ( 1997; ). Occurrence of arsenic-contaminated groundwater in alluvial aquifers from Delta Plains, eastern India: options for safe drinking water supply. Int J Water Resour Dev 13, 79–92.[CrossRef]
    [Google Scholar]
  3. Counsell, T. J. & Murray, R. G. E. ( 1986; ). Polar lipid profiles of the genus Deinococcus. Int J Syst Bacteriol 36, 202–206.[CrossRef]
    [Google Scholar]
  4. de Vicente, A., Avilès, M., Codina, J. C., Borrego, J. J. & Romero, P. ( 1990; ). Resistance to antibiotics and heavy metals of Pseudomonas aeruginosa isolated from natural waters. J Appl Bacteriol 68, 625–632.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Department of Genetics, University of Washington, Seattle, USA.
  6. Friedrich, W., Bormann, E. J. & Grafe, U. ( 1984; ). Isolation and biological properties of arsenate-resistant strains of Streptomyces noursei. Z Allg Mikrobiol 24, 13–19.[CrossRef]
    [Google Scholar]
  7. Gihring, T. M., Druschel, G. K., McCleskey, R. B., Hamers, R. J. & Banfield, J. F. ( 2001; ). Rapid arsenite oxidation by Thermus aquaticus and Thermus thermophilus: field and laboratory investigations. Environ Sci Technol 35, 3857–3862.[CrossRef]
    [Google Scholar]
  8. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  9. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19, 161–206.
    [Google Scholar]
  10. Laemmli, U. K. ( 1970; ). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef]
    [Google Scholar]
  11. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  12. Murray, R. G. E. & Brooks, B. W. ( 1986; ). Genus I. Deinococcus Brooks and Murray 1981, 354VP. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1035–1043. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  13. Nickson, R., McArthur, J., Burgess, W., Ahmed, K. M., Ravenscroft, P. & Rahman, M. ( 1998; ). Arsenic poisoning of Bangladesh groundwater. Nature 395, 338.[CrossRef]
    [Google Scholar]
  14. Niggemyer, A., Spring, S., Stackebrandt, E. & Rosenzweig, R. F. ( 2001; ). Isolation and characterization of a novel As(V)-reducing bacterium: implications for arsenic mobilization and the genus Desulfitobacterium. Appl Environ Microbiol 67, 5568–5580.[CrossRef]
    [Google Scholar]
  15. Oyaizu, H., Stackebrandt, E., Schleifer, K. H., Ludwig, W., Pohla, H., Ito, H., Hirata, A., Oyaizu, Y. & Komagata, K. ( 1987; ). A radiation-resistant rod-shaped bacterium, Deinobacter grandis gen. nov., sp. nov., with peptidoglycan containing ornithine. Int J Syst Bacteriol 37, 62–67.[CrossRef]
    [Google Scholar]
  16. Prithivirajsingh, S., Mishra, S. K. & Mahadevan, A. ( 2001; ). Detection and analysis of chromosomal arsenic resistance in Pseudomonas fluorescens strain MSP3. Biochem Biophys Res Commun 280, 1393–1401.[CrossRef]
    [Google Scholar]
  17. Rainey, F. A., Nobre, M. F., Schumann, P., Stackebrandt, E. & Da Costa, M. S. ( 1997; ). Phylogenetic diversity of the deinococci as determined by 16S ribosomal DNA sequence comparison. Int J Syst Bacteriol 47, 510–514.[CrossRef]
    [Google Scholar]
  18. Reddy, G. S. N., Aggarwal, R. K., Matsumoto, G. I. & Shivaji, S. ( 2000; ). Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica. Int J Syst Evol Microbiol 50, 1553–1561.[CrossRef]
    [Google Scholar]
  19. Reddy, G. S. N., Prakash, J. S. S., Matsumoto, G. I., Stackebrandt, E. & Shivaji, S. ( 2002a; ). Arthrobacter roseus sp. nov. a psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 52, 1017–1021.[CrossRef]
    [Google Scholar]
  20. Reddy, G. S. N., Prakash, J. S. S., Vairamani, M., Prabhakar, S., Matsumoto, G. I. & Shivaji, S. ( 2002b; ). Planococcus antarcticus and Planococcus psychrophilus spp. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. Extremophiles 6, 253–261.[CrossRef]
    [Google Scholar]
  21. Reddy, G. S. N., Matsumoto, G. I. & Shivaji, S. ( 2003; ). Sporosarcina macmurdoensis sp. nov., from a cyanobacterial mat sample from a pond in the McMurdo Dry Valleys, Antarctica. Int J Syst Evol Microbiol 53, 1363–1367.[CrossRef]
    [Google Scholar]
  22. Sato, N. S. & Murata, N. ( 1988; ). Membrane lipids. Methods Enzymol 167, 251–259.
    [Google Scholar]
  23. Sato, T. & Kobayashi, Y. ( 1998; ). The ars operon in the skin element of Bacillus subtilis confers resistance to arsenate and arsenite. J Bacteriol 180, 1655–1661.
    [Google Scholar]
  24. Shivaji, S., Rao, N. S., Saisree, L., Reddy, G. S. N., Seshu Kumar, G. & Bhargava, P. M. ( 1989; ). Isolates of Arthrobacter from the soils of Schirmacher Oasis, Antarctica. Polar Biol 10, 225–229.
    [Google Scholar]
  25. Shivaji, S., Ray, M. K., Rao, N. S., Saisree, L., Jagannadham, M. V., Seshu Kumar, G., Reddy, G. S. N. & Bhargava, P. M. ( 1992; ). Sphingobacterium antarcticus sp. nov., a psychrotrophic bacterium from the soils of Schirmacher Oasis, Antarctica. Int J Syst Bacteriol 42, 102–106.[CrossRef]
    [Google Scholar]
  26. Shivaji, S., Vijaya Bhanu, N. & Aggarwal, R. K. ( 2000; ). Identification of Yersinia pestis as the causative organism of plague in India as determined by 16S rDNA sequencing and RAPD-based genomic fingerprinting. FEMS Microbiol Lett 189, 247–252.[CrossRef]
    [Google Scholar]
  27. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–655. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  28. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  29. Tourova, T. P. & Antonov, A. S. ( 1987; ). Identification of microorganisms by rapid DNA–DNA hybridisation. Methods Microbiol 19, 333–355.
    [Google Scholar]
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vol. , part 2, pp. 457 – 461

Percentage survival of colonies when exposed to UV radiation.

One-dimensional TLC of total lipid extracts.

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