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Volume 61, Issue 10

gen. nov., sp. nov., a marine bacterium isolated from a coral reef sea squirt, and description of fam. nov. Free

Abstract

Two aerobic, Gram-reaction-negative, golden-yellow pigmented and rod-shaped bacteria, designated strains A5Q-118 and A5Q-27, were isolated from an unidentified sea squirt that thrives in the coral reefs off the coast of Okinawa, Japan. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the novel isolates were affiliated with the family ‘’ of the phylum . Strains A5Q-118 and A5Q-27 shared 100 % sequence similarity with each other and showed <92 % similarity with other cultivated members of the family ‘’. The novel isolates were phenotypically and physiologically different from strains described previously. The DNA G+C content was 35.5–36.2 mol%, MK-7 was the major menaquinone and iso-C and Cω5 were the major fatty acids. Based on the results of this polyphasic taxonomic study, it was concluded that strains A5Q-118 and A5Q-27 represent a novel species in a new genus of the family ‘’, for which the name gen. nov., sp. nov. is proposed. Proposal for designation of the fam. nov. is also presented. The type strain of is A5Q-118 ( = KCTC 23232  = NBRC 107587).

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Funding
This study was supported by the:
  • New Energy and Industrial Technology Development Organization (NEDO)
  • Institute for Fermentation (Award 2009-2011)
© 2011 IUMS
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2011-10-01
2023-12-01
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References

  1. Bowman J. P., McCammon S. A., Brown M. V., Nichols D. S., McMeekin T. A. 1997; Diversity and association of psychrophilic bacteria in Antarctic sea ice. Appl Environ Microbiol 63:3068–3078[PubMed]
     [Google Scholar]
  2. Cottrell M. T., Kirchman D. L. 2000; Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization. Appl Environ Microbiol 66:5116–5122 [View Article][PubMed]
     [Google Scholar]
  3. DeLong E. F., Franks D. G., Alldredge A. L. 1993; Phylogenetic diversity of aggregate-attached vs. free-living marine bacterial assemblages. Limnol Oceanogr 38:924–934 [View Article]
     [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  5. Fitch W. M. 1971; Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
     [Google Scholar]
  6. Glöckner F. O., Fuchs B. M., Amann R. 1999; Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl Environ Microbiol 65:3721–3726[PubMed]
     [Google Scholar]
  7. Hosoya S., Yokota A. 2007; Flammeovirga kamogawensis sp. nov., isolated from coastal seawater in Japan. Int J Syst Evol Microbiol 57:1327–1330 [View Article][PubMed]
     [Google Scholar]
  8. Katayama-Fujimura Y., Komatsu Y., Kuraishi H., Kaneko T. 1984; Estimation of DNA base composition by high performance liquid chromatography of its nucleae P1 hydrolysate. Agric Biol Chem 48:3169–3172 [View Article]
     [Google Scholar]
  9. Khan S. T., Nakagawa Y., Harayama S. 2007; Sediminitomix flava gen. nov., sp. nov., of the phylum Bacteroidetes, isolated from marine sediment. Int J Syst Evol Microbiol 57:1689–1693 [View Article][PubMed]
     [Google Scholar]
  10. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef]
     [Google Scholar]
  11. Lewin R. A. 1970; Flexithrix dorotheae gen. et sp. nov. (Flexibacterales); and suggestions for reclassifying sheathed bacteria. Can J Microbiol 16:511–515 [View Article]
     [Google Scholar]
  12. Ludwig W., Klenk H.-P. 2001; Overview: a phylogenetic backbone and taxonomic framework for prokaryotic systematics. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 1 pp. 49–66 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer; [View Article]
     [Google Scholar]
  13. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
     [Google Scholar]
  14. Muramatsu Y., Takahashi M., Kaneyasu M., Iino T., Suzuki K., Nakagawa Y. 2010; Persicobacter psychrovividus sp. nov., isolated from shellfish, and emended descriptions of the genus Persicobacter and Persicobacter diffluens . Int J Syst Evol Microbiol 60:1735–1739 [View Article][PubMed]
     [Google Scholar]
  15. Murray R. G. E., Doetsch R. N., Robinow C. F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp. 21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  16. Nakagawa Y., Hamana K., Sakane T., Yamasato K. 1997; Reclassification of Cytophaga aprica (Lewin 1969) Reichenbach 1989 in Flammeovirga gen. nov. as Flammeovirga aprica comb. nov. and of Cytophaga diffuens (ex Stanier 1940; emend. Lewin 1969) Reichenbach 1989 in Persicobacter gen. nov. as Persicobacter diffuens comb. nov.. Int J Syst Bacteriol 47:220–223 [View Article]
     [Google Scholar]
  17. Nishijima M., Araki-Sakai M., Sano H. 1997; Identification of isoprenoid quinones by frit-FAB liquid chromatography-mass spectrometry for the chemotaxonomy of microorganisms. J Microbiol Methods 28:113–122 [View Article]
     [Google Scholar]
  18. O’Sullivan L. A., Weightman A. J., Fry J. C. 2002; New degenerate Cytophaga-Flexibacter-Bacteroides-specific 16S ribosomal DNA-targeted oligonucleotide probes reveal high bacterial diversity in River Taff epilithon. Appl Environ Microbiol 68:201–210 [View Article][PubMed]
     [Google Scholar]
  19. Perry L. B. 1973; Gliding motility in some non-spreading flexibacteria. J Appl Bacteriol 36:227–232[PubMed] [CrossRef]
     [Google Scholar]
  20. Pinhassi J., Zweifel U. L., Hagström Å. 1997; Dominant marine bacterioplankton species found among colony-forming bacteria. Appl Environ Microbiol 63:3359–3366[PubMed]
     [Google Scholar]
  21. Reichenbach H. 1989; Nonphotosynthetic, nonfruiting gliding bacteria. Genus 1. Cytophaga Winogradsky 1929, 577AL, emend. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 3 pp. 2015–2050 Edited by Staley J., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  22. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  23. Srisukchayakul P., Suwanachart C., Sangnoi Y., Kanjana-Opas A., Hosoya S., Yokota A., Arunpairojana V. 2007; Rapidithrix thailandica gen. nov., sp. nov., a marine gliding bacterium isolated from samples collected from the Andaman sea, along the southern coastline of Thailand. Int J Syst Evol Microbiol 57:2275–2279 [View Article][PubMed]
     [Google Scholar]
  24. Takahashi M., Suzuki K., Nakagawa Y. 2006; Emendation of the genus Flammeovirga and Flammeovirga aprica with the proposal of Flammeovirga arenaria nom. rev., comb. nov. and Flammeovirga yaeyamensis sp. nov.. Int J Syst Evol Microbiol 56:2095–2100 [View Article][PubMed]
     [Google Scholar]
  25. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  27. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703[PubMed]
     [Google Scholar]
  28. Yoon J., Ishikawa S., Kasai H., Yokota A. 2007; Perexilibacter aurantiacus gen. nov., sp. nov., a novel member of the family ‘Flammeovirgaceae’ isolated from sediment. Int J Syst Evol Microbiol 57:964–968 [View Article][PubMed]
     [Google Scholar]
  29. Yoon J., Matsuo Y., Kasai H., Yokota A. 2008; Limibacter armeniacum gen. nov., sp. nov., a novel representative of the family ‘Flammeovirgaceae’ isolated from marine sediment. Int J Syst Evol Microbiol 58:982–986 [View Article][PubMed]
     [Google Scholar]
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Aureibacter tunicatorum gen. nov., sp. nov., a marine bacterium isolated from a coral reef sea squirt, and description of Flammeovirgaceae fam. nov.
Int J Syst Evol Microbiol 61, 2342 (2011); https://doi.org/10.1099/ijs.0.027573-0
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