1887

Abstract

The taxonomic relationships of two actinomycetes provisionally assigned to the genus were determined using a polyphasic taxonomic approach. The generic assignment was confirmed by 16S rRNA gene similarity data, as the organisms, strains MTCC 1534 and W 4937, were shown to belong to the subclade. These organisms had phenotypic properties typical of rhodococci; they were aerobic, Gram-positive, weakly acid-fast actinomycetes that showed an elementary branching-rod–coccus growth cycle and contained -diaminopimelic acid, arabinose and galactose in whole-organism hydrolysates, -glycolated muramic acid residues, dehydrogenated menaquinones with eight isoprene units as the predominant isoprenologue and mycolic acids that co-migrated with those extracted from the type strain of . The strains had identical phenotypic profiles and belong to the same genomic species, albeit one distinguished from , with which they formed a distinct phyletic line. They were also distinguished from representatives of all of the species classified in the 16S rRNA gene tree using a set of phenotypic features. The genotypic and phenotypic data show that the strains merit recognition as a novel species of . The name proposed is sp. nov., with the type strain W 4937 (=DSM 44689=NCTC 13296).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02756-0
2004-03-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/2/ijs540407.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02756-0&mimeType=html&fmt=ahah

References

  1. Brenner D. J., McWhorter A. C., Leete Knutson J. K., Steigerwalt A. G. 1982; Escherichia vulneris : a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol 15:1133–1140
    [Google Scholar]
  2. Bunch A. W. 1998; Biotransformation of nitriles by rhodococci. Antonie van Leeuwenhoek 74:89–97 [CrossRef]
    [Google Scholar]
  3. Chun J. 1995; Computer-assisted classification and identification of actinomycetes . PhD thesis University of Newcastle; Newcastle upon Tyne, UK:
  4. Collins M. D. 1994; Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics pp  265–309 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  5. Cuello O. H., Caorlin M. J., Reviglio V. E., Carvajal L., Juarez C. P., Palacio de Guerra E., Luna J. D. 2002; Rhodococcus globerulus keratitis after laser in situ keratomileusis. J Cataract Refract Surg 28:2235–2237 [CrossRef]
    [Google Scholar]
  6. Dabbs E. R. 1998; Cloning of genes that have environmental and clinical importance from rhodococci and related bacteria. Antonie van Leeuwenhoek 74:155–167 [CrossRef]
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1993 phylip (Phylogenetic Inference Package) version 3.5c. Distributed by the author Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  9. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284 [CrossRef]
    [Google Scholar]
  10. Goodfellow M., Thomas E. G., Ward A. C., James A. L. 1990; Classification and identification of rhodococci. Zentbl Bakteriol 274:299–315 [CrossRef]
    [Google Scholar]
  11. Goodfellow M., Alderson G., Chun J. 1998; Rhodococcal systematics: problems and developments. Antonie van Leeuwenhoek 74:1–12 [CrossRef]
    [Google Scholar]
  12. Goodfellow M., Isik K., Yates E. 1999; Actinomycete systematics: an unfinished synthesis. Nova Acta Leopold NF80:31247–82
    [Google Scholar]
  13. Goodfellow M., Chun J., Stackebrandt E., Kroppenstedt R. M. 2002; Transfer of Tsukamurella wratislaviensis Goodfellow et al . 1995 to the genus Rhodococcus as Rhodococcus wratislaviensis comb. nov. Int J Syst Evol Microbiol 52:749–755 [CrossRef]
    [Google Scholar]
  14. Goodfellow M., Jones A. L., Maldonado L. A., Salanitro J. 2004; Rhodococcus aetherivorans sp. nov., a new species of methyl t-butyl ether-degrading actinomycetes. Syst Appl Microbiol (in press
    [Google Scholar]
  15. Gordon R. E., Mihm J. M. 1962; Identification of Nocardia caviae nov. comb. Ann NY Acad Sci 98:628–636
    [Google Scholar]
  16. Graves L. M., Swaminathan B. 1993; Universal bacterial DNA isolation procedure. In Diagnostic Molecular Microbiology, Principles and Applications pp  617–621 Edited by Persing D. H., Smith T. F., Tenover F. C., White T. J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  18. Kedlaya I., Ing M. B., Wong S. S. 2001; Rhodococcus equi infections in immunocompetent hosts: case report and review. Clin Infect Dis 32:e39–e47 [CrossRef]
    [Google Scholar]
  19. Kim S. B., Falconer C., Williams E., Goodfellow M. 1998; Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil. Int J Syst Bacteriol 48:59–68 [CrossRef]
    [Google Scholar]
  20. Klugge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  21. Loeffelholz M. J., Scholl D. R. 1989; Method for improved extraction of DNA from Nocardia asteroides . J Clin Microbiol 27:1880–1881
    [Google Scholar]
  22. McMinn E. J., Alderson G., Dodson H. I., Goodfellow M., Ward A. C. 2000; Genomic and phenomic differentiation of Rhodococcus equi and related strains. Antonie van Leeuwenhoek 78:331–340 [CrossRef]
    [Google Scholar]
  23. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid containing bacteria. J Chromatogr 188:221–233 [CrossRef]
    [Google Scholar]
  24. Rainey F. A., Burghardt J., Kroppenstedt R. M., Klatte S., Stackebrandt E. 1995; Phylogenetic analysis of the genera Rhodococcus and Nocardia and evidence for the evolutionary origins of the genus Nocardia from within the radiation of Rhodococcus species. Microbiology 141:523–528 [CrossRef]
    [Google Scholar]
  25. Rowbotham T. J., Cross T. 1977; Ecology of Rhodococcus coprophilus and associated actinomycetes in freshwater and agricultural habitats. J Gen Microbiol 100:231–240 [CrossRef]
    [Google Scholar]
  26. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  27. Schaal K. P. 1985; Identification of clinically significant actinomycetes and related bacteria using chemical techniques. In Chemical Methods in Bacterial Systematics pp  359–381 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  28. Spark R. P., McNeil M. M., Brown J. M., Lasker B. A., Montano M. A., Garfield M. D. 1993; Rhodococcus species fatal infection in an immunocompetent host. Arch Pathol Lab Med 117:515–520
    [Google Scholar]
  29. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  30. Takeuchi M., Hatano K., Sedlácek I., Pácová Z. 2002; Rhodococcus jostii sp. nov., isolated from a medieval grave. Int J Syst Evol Microbiol 52:409–413
    [Google Scholar]
  31. Tsukamura M., Yano I., Kudo T., Miyama A. 1991; Rhodococcus roseus sp. nov., nom. rev. Int J Syst Bacteriol 41:385–389 [CrossRef]
    [Google Scholar]
  32. Uchida K., Kudo T., Suzuki K., Nakase T. 1999; A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56 [CrossRef]
    [Google Scholar]
  33. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  34. Weinstock D. M., Brown A. E. 2002; Rhodococcus equi : an emerging pathogen. Clin Infect Dis 34:1379–1385 [CrossRef]
    [Google Scholar]
  35. Yoon J.-H., Kang S.-S., Cho Y.-G., Lee S. T., Kho Y. H., Kim C.-J., Park Y.-H. 2000; Rhodococcus pyridinivorans sp. nov., a pyridine-degrading bacterium. Int J Syst Evol Microbiol 50:2173–2180 [CrossRef]
    [Google Scholar]
  36. Zhang J., Zhang Y., Xiao C., Liu Z., Goodfellow M. 2002; Rhodococcus maanshanensis sp. nov., a novel actinomycete from soil. Int J Syst Evol Microbiol 52:2121–2126 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02756-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02756-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error