1887

Abstract

A Gram-staining-positive coccus, designated CC-SPL15-2, was isolated from the rhizosphere of . By 16S rRNA gene sequence analysis, it was shown that strain CC-SPL15-2 belonged to the genus . The isolate was most closely related to DSM 5352 (98.3 % 16S rRNA gene sequence similarity) and DSM 5351 (96.7 %); similarities to all other members of the genus were <96.5 %. In accordance with characteristics of the genus , the quinone system was mainly composed of menaquinone MK-6. The polar lipid profile exhibited the major components diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. In the polyamine pattern, spermidine was the predominant compound. The fatty acids were anteiso-C, iso-C, iso-C and anteiso-C, which supported the affiliation of strain CC-SPL15-2 to the genus . DNA–DNA relatedness between strain CC-SPL15-2 and CCUG 43288 was 42 and 32 % (reciprocal analysis). From these data as well as from physiological and biochemical tests, a clear differentiation of strain CC-SPL15-2 from and other members of the genus was possible. We propose that strain CC-SPL15-2 be assigned to a novel species, with the name sp. nov. The type strain is CC-SPL15-2 ( = DSM 23267  = CCM 7756).

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2011-10-01
2019-10-19
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vol. , part 10, pp. 2348–2352

Polar lipid profile of sp. nov. CC-SPL15-2 after separation by two-dimensional TLC and detection with molybdatophosphoric acid. DPG, Diphosphatidylglycerol; GL, unidentified glycolipid; L, unidentified lipid not reacting with any of the specific spray reagents used to detect amino, phosphate or sugar moieties; PG, phosphatidylglycerol.



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