1887

Abstract

A bacterial strain, designated AST4, was isolated from activated sludge. The bacterium did not show significant growth on nutrient broth, but growth was clearly stimulated by addition of supernatant from other bacterial cultures. Culture filtrate of a strain related to the genus in particular increased the cell yield and growth rate of strain AST4. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain AST4 is located within the ‘ group’ in the -3 subclass of , but is clearly distant from related genera in this group such as , and . Strain AST4 is a Gram-negative, non-motile, rod-shaped (0·6–0·8×1·3–2·0 μm) and aerobic bacterium. It was not able to reduce nitrate to nitrite or N. No phototrophic growth was observed. Optimal growth occurred at 30 °C and pH 6·5–7·5. The dominant cellular fatty acid in the isolate was C 11. Ubiquinone-10 was the major respiratory quinone. The G+C content was 64·5 mol% (by HPLC). Based on the phylogenetic and phenotypic traits, the name gen. nov., sp. nov. is proposed for this isolate; the type strain is AST4 (=NBRC 100046=JCM 11959=DSM 15620).

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2004-05-01
2019-09-16
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vol. , part 3, pp. 955 - 959

Phylogenetic tree of strain AST4 and all species in the genera , and based on their 16S rRNA gene sequences. Bootstrap percentages are indicated at branching points. Bar, 1 substitution in 100 nt. Accession numbers of reference sequences are shown in parentheses. [PDF](100 KB)



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