sp. nov., a round-spore-forming bacillus isolated from the Mars Odyssey spacecraft Free

Abstract

A round-spore-forming species that produces an exosporium was isolated from the surface of the Mars Odyssey spacecraft. This novel species has been characterized on the basis of phenotypic traits, 16S rDNA sequence analysis and DNA–DNA hybridization. According to the results of these analyses, this strain belongs to the genus and is a Gram-positive, aerobic, rod-shaped, endospore-forming eubacterium. Ultrathin sections of the spores showed the presence of an exosporium, spore coat, cortex and core. 16S rDNA sequence similarities between this strain, and were ∼96 % and DNA–DNA reassociation values with these two bacilli were 23 and 17 %, respectively. Spores of the novel species were resistant to desiccation, HO and UV and gamma radiation. Of all strains tested, the spores of this strain were the most consistently resistant and survived all of the challenges posed, i.e. exposure to conditions of desiccation (100 % survival), HO (26 % survival), UV radiation (10 % survival at 660 J m) and gamma radiation (0·4 % survival). The name proposed for this novel bacterium is sp. nov.; the type strain is 34hs-1 (=ATCC PTA-4993=NRRL B-30641=NBRC 100172).

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2004-01-01
2024-03-29
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References

  1. Anonymous. 1980 NASA Standard Procedures for the Microbiological Examination of Space Hardware , NHB 5340.1B Pasadena, CA: National Aeronautics and Space Administration;
    [Google Scholar]
  2. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett Appl Microbiol 13:202–206
    [Google Scholar]
  3. Chester F. D. 1898; Report of the mycologist: bacteriological work. Del Agric Exp Stn Annu Rep 10:47–137
    [Google Scholar]
  4. Cole R. M., Popkin T. J. 1981; Electron microscopy. In Manual of Methods for General Bacteriology pp  34–51 Edited by Gerhardt P., Murray R. G. E., Costilaw R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Colwell R. R., Grimes D. J. 2000 Nonculturable Microorganisms in the Environment Washington, DC: American Society for Microbiology;
    [Google Scholar]
  6. Coss J. R. 1999 Test Procedure for Total Ionizing Dose Radiation Testing of Piece-Parts #D-15827 Pasadena, CA: National Aeronautics and Space Administration;
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  8. Johnson J. L. 1981; Genetic characterization. In Manual of Methods for General Bacteriology pp  450–472 Edited by Gerhardt P., Murray R. G. E., Costilaw R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  9. La Duc M. T., Nicholson W., Kern R., Venkateswaran K. 2003; Microbial characterization of the Mars Odyssey spacecraft and its encapsulation facility. Environ Microbiol 5:977–985 [CrossRef]
    [Google Scholar]
  10. Nakamura L. K. 2000; Phylogeny of Bacillus sphaericus -like organisms. Int J Syst Evol Microbiol 50:1715–1722
    [Google Scholar]
  11. Nakamura L. K., Shida O., Takagi H., Komagata K. 2002; Bacillus pycnus sp. nov. and Bacillus neidei sp. nov., round-spored bacteria from soil. Int J Syst Evol Microbiol 52:501–505
    [Google Scholar]
  12. Neide E. 1904; Botanische Beschreibung einiger sporenbild- enden Bakterien. Zentbl Bakteriol Parasitenkd Infektionskr Hyg Abt II 12337–352
    [Google Scholar]
  13. Nicholson W. L., Setlow P. 1990; Sporulation, germination, and outgrowth. In Molecular Biological Methods for Bacillus pp  391–450 Edited by Harwood C. R., Cutting S. M. Chichester: Wiley;
    [Google Scholar]
  14. Nicholson W. L., Munakata N., Horneck G., Melosh H. J., Setlow P. 2000; Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol Mol Biol Rev 64:548–572 [CrossRef]
    [Google Scholar]
  15. Priest F. G. 1993; Systematics and ecology of Bacillus . In Bacillus subtilis and Other Gram-positive Bacteria . pp  3–33 Edited by Sonenshein A. L., Hoch J. A., Losick R. Washington, DC: American Society for Microbiology;
  16. Priest F. G., Goodfellow M., Todd C. 1988; A numerical classification of the genus Bacillu s. J Gen Microbiol 134:1847–1882
    [Google Scholar]
  17. Rheims H., Fruhling A., Schumann P., Rohde M., Stackebrandt E. 1999; Bacillus silvestris sp. nov., a new member of the genus Bacillus that contains lysine in its cell wall. Int J Syst Bacteriol 49:795–802 [CrossRef]
    [Google Scholar]
  18. Riesenman P. J., Nicholson W. L. 2000; Role of the spore coat layers in Bacillus subtilis spore resistance to hydrogen peroxide, artificial UV-C, UV-B, and solar UV radiation. Appl Environ Microbiol 66:620–626 [CrossRef]
    [Google Scholar]
  19. Rüger H. J., Fritze D., Spröer C. 2000; New psychrophilic and psychrotolerant Bacillus marinus strains from tropical and polar deep-sea sediments and emended description of the species. Int J Syst Evol Microbiol 50:1305–1313 [CrossRef]
    [Google Scholar]
  20. Ruimy R., Breittmayer V., Elbaze P., Lafay B., Boussemart O., Gauthier M., Christen R. 1994; Phylogenetic analysis and assessment of the genera Vibrio , Photobacterium , Aeromonas , and Plesiomonas deduced from small-subunit rRNA sequences. Int J Syst Bacteriol 44:416–426 [CrossRef]
    [Google Scholar]
  21. Satomi M., Kimura B., Mizoi M., Sato T., Fujii T. 1997; Tetragenococcus muriaticus sp. nov., a new moderately halophilic lactic acid bacterium isolated from fermented squid liver sauce. Int J Syst Bacteriol 47:832–836 [CrossRef]
    [Google Scholar]
  22. Schaeffer P., Millet J., Aubert J.-P. 1965; Catabolic repression of bacterial sporulation. Proc Natl Acad Sci U S A 54:704–711 [CrossRef]
    [Google Scholar]
  23. Swofford D. 1990 paup: phylogenetic analysis using parsimony, version 3.0 Illinois Natural History Survey; Champaign, IL, USA:
    [Google Scholar]
  24. Venkateswaran K., Satomi M., Chung S., Kern R., Koukol R., Basic C., White D. 2001; Molecular microbial diversity of a spacecraft assembly facility. Syst Appl Microbiol 24:311–320 [CrossRef]
    [Google Scholar]
  25. Venkateswaran K., Kempf M., Chen F., Satomi M., Nicholson W., Kern R. 2003; Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are γ -radiation resistant. Int J Syst Evol Microbiol 53:165–172 [CrossRef]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  27. Yoon J.-H., Lee K.-C., Weiss N., Kho Y. H., Kang K. H., Park Y.-H. 2001; Sporosarcina aquimarina sp. nov., a bacterium isolated from seawater in Korea, and transfer of Bacillus globisporus (Larkin and Stokes 1967), Bacillus psychrophilus (Nakamura 1984) and Bacillus pasteurii (Chester 1898) to the genus Sporosarcina as Sporosarcina globispora comb.nov., Sporosarcina psychrophila comb. nov. and Sporosarcina pasteurii comb. nov., and emended description of the genus Sporosarcina . Int J Syst Evol Microbiol 51:1079–1086 [CrossRef]
    [Google Scholar]
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