1887

Abstract

A strictly aerobic, nitrate-reducing, motile, rod-shaped member of the class , designated strain JC2671, was isolated from a seashore sand sample from Dokdo, Korea. The isolate reduced nitrate to nitrite, but not to nitrogen, and required NaCl for growth. 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus within the order with sequence similarities of 96.0–97.6 % to strains of species with validly published names. However, in DNA–DNA hybridization studies, a low genomic relatedness (43 %) between strain JC2671 and the type strain of indicated that the isolate represented a novel genomic species. The polar lipid pattern (phosphatidylethanolamine and phosphatidylglycerol), predominant cellular fatty acids [C, summed feature 3 (Cω6 and/or Cω7) and summed feature 8 (Cω6 and/or Cω7)] and the DNA G+C content (59 mol%) of the novel strain were consistent with its assignment to the genus . In contrast, a number of phenotypic characteristics, namely a requirement of NaCl for growth, the inability to grow under facultatively anaerobic conditions, the absence of nitrite reduction and differences in carbohydrate utilization and enzymic activities, clearly distinguished the novel isolate from other species of the genus . Data from this polyphasic study indicate that strain JC2671 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is JC2671 ( = KACC 15081  = JCM 17110). The description of the genus has been emended accordingly.

Funding
This study was supported by the:
  • , Priority Research Centers Program , (Award 2008-005J00201)
  • , National Research Foundation , (Award 2010-0017955)
  • , the Korean Government (MEST)
  • , Regional SubGenBank Support Program of Rural Development Administration, Republic of Korea
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2011-10-01
2020-11-30
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References

  1. Bowman J. P., McMeekin T. A. 2005; Order X. Alteromonadales ord. nov.. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2 pp. 443 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  2. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [CrossRef][PubMed]
    [Google Scholar]
  3. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef][PubMed]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1993; phylip (phylogenetic inference package) version 3.5.1. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA
  6. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  7. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  8. Jeon Y. S., Chung H., Park S., Hur I., Lee J. H., Chun J. 2005; jphydit: a java-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21:3171–3173 [CrossRef][PubMed]
    [Google Scholar]
  9. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp. 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  10. Lin Y. T., Shieh W. Y. 2006; Zobellella denitrificans gen. nov., sp. nov. and Zobellella taiwanensis sp. nov., denitrifying bacteria capable of fermentative metabolism. Int J Syst Evol Microbiol 56:1209–1215 [CrossRef]
    [Google Scholar]
  11. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  12. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  13. Ronquist F., Huelsenbeck J. P. 2003; MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574 [CrossRef][PubMed]
    [Google Scholar]
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  15. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society forMicrobiology;
    [Google Scholar]
  16. Swofford D. L. 1998; Phylogenetic analysis using parsimony (paup), version 4. Sunderland, MA: Sinauer Associates;
  17. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  18. Yi H., Chun J. 2006; Thalassobius aestuarii sp. nov., isolated from tidal flat sediment. J Microbiol 44:171–176[PubMed]
    [Google Scholar]
  19. ZoBell C. E. 1941; Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4:42–75
    [Google Scholar]
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