1887

Abstract

A strictly aerobic, nitrate-reducing, motile, rod-shaped member of the class , designated strain JC2671, was isolated from a seashore sand sample from Dokdo, Korea. The isolate reduced nitrate to nitrite, but not to nitrogen, and required NaCl for growth. 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus within the order with sequence similarities of 96.0–97.6 % to strains of species with validly published names. However, in DNA–DNA hybridization studies, a low genomic relatedness (43 %) between strain JC2671 and the type strain of indicated that the isolate represented a novel genomic species. The polar lipid pattern (phosphatidylethanolamine and phosphatidylglycerol), predominant cellular fatty acids [C, summed feature 3 (Cω6 and/or Cω7) and summed feature 8 (Cω6 and/or Cω7)] and the DNA G+C content (59 mol%) of the novel strain were consistent with its assignment to the genus . In contrast, a number of phenotypic characteristics, namely a requirement of NaCl for growth, the inability to grow under facultatively anaerobic conditions, the absence of nitrite reduction and differences in carbohydrate utilization and enzymic activities, clearly distinguished the novel isolate from other species of the genus . Data from this polyphasic study indicate that strain JC2671 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is JC2671 ( = KACC 15081  = JCM 17110). The description of the genus has been emended accordingly.

Funding
This study was supported by the:
  • Priority Research Centers Program (Award 2008-005J00201)
  • National Research Foundation (Award 2010-0017955)
  • the Korean Government (MEST)
  • Regional SubGenBank Support Program of Rural Development Administration, Republic of Korea
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2011-10-01
2024-11-11
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