1887

Abstract

An orange-coloured, rod-shaped, non-motile, Gram-reaction-negative, strictly aerobic bacterial strain, designated JC2680, was isolated from a seawater sample of Jeju Island, Korea. The isolate required sea salts for growth. Flexirubin-type pigments were absent. 16S rRNA gene sequence analysis indicated that the test strain belonged to the genus within the family , but shared relatively low levels of similarity (93.6–95.9 %) with the type strains of recognized species. The predominant cellular fatty acids [iso-C 3-OH, iso-C and summed feature 9 (comprising 10-methyl C and/or iso-Cω9) and DNA G+C content (35 mol%) were consistent with the assignment of strain JC2680 to the genus . However, a number of phenotypic characteristics, namely inability to grow under microaerophilic conditions, differences in enzyme reactions, and absence of flexirubin-type pigments and gliding motility, clearly distinguished strain JC2680 from recognized species of the genus . The data presented thus indicate that strain JC2680 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC2680 ( = KACC 14156  = JCM 17106).

Funding
This study was supported by the:
  • Priority Research Centers Program (Award 2008-005J00201)
  • National Research Foundation (Award 2010-0017955)
  • Korean government (MEST)
  • National Institute of Biological Resources (NIBR)
  • Ministry of Environment
  • Regional SubGenBank Support Program of Rural Development Administration, Republic of Korea
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.027433-0
2011-10-01
2024-10-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/10/2445.html?itemId=/content/journal/ijsem/10.1099/ijs.0.027433-0&mimeType=html&fmt=ahah

References

  1. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  2. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:1861–1868[PubMed]
    [Google Scholar]
  3. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [View Article][PubMed]
    [Google Scholar]
  4. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  6. Felsenstein J. 1993; phylip (phylogenetic inference package) version 3.5.1. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA
  7. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [View Article]
    [Google Scholar]
  8. Jeon Y. S., Chung H., Park S., Hur I., Lee J. H., Chun J. 2005; jphydit: a JAVA-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21:3171–3173 [View Article][PubMed]
    [Google Scholar]
  9. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp. 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  10. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  11. Miyazaki M., Nagano Y., Fujiwara Y., Hatada Y., Nogi Y. 2010; Aquimarina macrocephali sp. nov., isolated from sediment adjacent to sperm whale carcasses. Int J Syst Evol Microbiol 60:2298–2302 [View Article][PubMed]
    [Google Scholar]
  12. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Frolova G. M., Mikhailov V. V., Lee K. H., Bae K. S. 2005; Description of Aquimarina muelleri gen. nov., sp. nov., and proposal of the reclassification of [Cytophaga] latercula Lewin 1969 as Stanierella latercula gen. nov., comb. nov.. Int J Syst Evol Microbiol 55:225–229 [View Article][PubMed]
    [Google Scholar]
  13. Nedashkovskaya O. I., Vancanneyt M., Christiaens L., Kalinovskaya N. I., Mikhailov V. V., Swings J. 2006; Aquimarina intermedia sp. nov., reclassification of Stanierella latercula (Lewin 1969) as Aquimarina latercula comb. nov. and Gaetbulimicrobium brevivitae Yoon et al. 2006 as Aquimarina brevivitae comb. nov. and emended description of the genus Aquimarina . Int J Syst Evol Microbiol 56:2037–2041 [View Article][PubMed]
    [Google Scholar]
  14. Ronquist F., Huelsenbeck J. P. 2003; MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574 [View Article][PubMed]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  16. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  18. Swofford D. L. 1998; Phylogenetic analysis using parsimony (paup), version 4. Sunderland, MA: Sinauer Associates;
  19. Weeks O. B. 1981; Preliminary studies of the pigments of Flavobacterium breve NCTC 11099 and Flavobacterium odoratum NCTC 11036. In The Flavobacterium-Cytophaga Group pp. 108–114 Edited by Reichenbach H., Weeks O. B. Weinheim: Gesellschaft für Biotechnologische Forschung;
    [Google Scholar]
  20. Yoon J. H., Kang S. J., Jung S. Y., Oh H. W., Oh T. K. 2006; Gaetbulimicrobium brevivitae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 56:115–119 [View Article][PubMed]
    [Google Scholar]
  21. Yoon B. J., You H. S., Lee D. H., Oh D. C. 2011; Aquimarina spongiae sp. nov., isolated from marine sponge Halichondria oshoro . Int J Syst Evol Microbiol 61:417–421 [View Article][PubMed]
    [Google Scholar]
  22. ZoBell C. E. 1941; Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4:42–75
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.027433-0
Loading
/content/journal/ijsem/10.1099/ijs.0.027433-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error