1887

Abstract

The third biovariant complex (sorbitol-negative and sorbitol-positive) contains unnamed taxa first characterized in 1991. These organisms can cause respiratory infections, a spectrum of soft tissue and skeletal infections, bacteraemia and disseminated disease. To evaluate this group of organisms, clinical reference isolates and the type strains of third biovariant complex sorbitol-negative (=21), third biovariant complex sorbitol-positive (=3), (=3), (pipemidic acid-susceptible) (=1), (=1), (=2) and (=1) were characterized by using conventional phenotypic (morphological, physiological and antimicrobial susceptibilities), chemotaxonomic (HPLC and cellular fatty acids) and genotypic [RFLP of the rRNA gene (ribotyping), PCR-RFLP of a 439 bp segment of the 65 kDa gene (PCR restriction analysis) and 16S rRNA gene sequence] analysis, DNA G+C content and DNA–DNA relatedness analyses. The results of these studies indicated that the strains comprised (=13), (=1) and four novel closely related genetic groups within the third biovariant complex: sp. nov. (=6), sp. nov. (=2), sp. nov. (=1) and sp. nov. (=1), with type strains ATCC 49935 (=W5998=DSM 44677), ATCC 49403 (=W5198=DSM 44676) ATCC 49404 (=W6705=DSM 44679) and ATCC 49938 (=W6743=DSM 44680), respectively.

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2004-09-01
2019-09-21
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Ribotype (RT) groups and DNA relatedness of representative strains used in this study. [PDF](24 KB)

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Signature nucleotides within the hypervariable region A of the 16S rRNA gene for species using as the reference species. The first nucleotide corresponds to position 175. Dots indicate positions that were identical to the sequence. GS, Genomospecies. [PDF](44 KB)

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