1887

Abstract

The third biovariant complex (sorbitol-negative and sorbitol-positive) contains unnamed taxa first characterized in 1991. These organisms can cause respiratory infections, a spectrum of soft tissue and skeletal infections, bacteraemia and disseminated disease. To evaluate this group of organisms, clinical reference isolates and the type strains of third biovariant complex sorbitol-negative (=21), third biovariant complex sorbitol-positive (=3), (=3), (pipemidic acid-susceptible) (=1), (=1), (=2) and (=1) were characterized by using conventional phenotypic (morphological, physiological and antimicrobial susceptibilities), chemotaxonomic (HPLC and cellular fatty acids) and genotypic [RFLP of the rRNA gene (ribotyping), PCR-RFLP of a 439 bp segment of the 65 kDa gene (PCR restriction analysis) and 16S rRNA gene sequence] analysis, DNA G+C content and DNA–DNA relatedness analyses. The results of these studies indicated that the strains comprised (=13), (=1) and four novel closely related genetic groups within the third biovariant complex: sp. nov. (=6), sp. nov. (=2), sp. nov. (=1) and sp. nov. (=1), with type strains ATCC 49935 (=W5998=DSM 44677), ATCC 49403 (=W5198=DSM 44676) ATCC 49404 (=W6705=DSM 44679) and ATCC 49938 (=W6743=DSM 44680), respectively.

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2004-09-01
2021-02-28
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References

  1. Barnes W. M. 1994; PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc Natl Acad Sci U S A 91:2216–2220 [CrossRef]
    [Google Scholar]
  2. Berd D. 1973; Laboratory identification of clinically important aerobic actinomycetes. Appl Microbiol 25:665–681
    [Google Scholar]
  3. Brenner D. J., McWhorter A. C., Knutson J. K., Steigerwalt A. G. 1982; Escherichia vulneris : a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol 15:1133–1140
    [Google Scholar]
  4. Brenner D. J., Hickman-Brenner F. W., Lee J. V. 7 other authors 1983; Vibrio furnissii (formerly aerogenic biogroup of Vibrio fluvialis ), a new species isolated from human feces and the environment. J Clin Microbiol 18:816–824
    [Google Scholar]
  5. Brenner D. J., Grimont P. A., Steigerwalt A. G., Fanning G. R., Ageron E., Riddle C. F. 1993; Classification of citrobacteria by DNA hybridization: designation of Citrobacter farmeri sp.nov., Citrobacter youngae sp. nov.,Citrobacter braakii sp. nov., Citrobacter werkmanii sp. nov., Citrobacter sedlakii sp.nov., and three unnamed Citrobacter genomospecies. Int J Syst Bacteriol 43:645–658 [CrossRef]
    [Google Scholar]
  6. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  7. Butler W. R., Jost K. C. Jr, Kilburn J. O. 1991; Identification of mycobacteria by high-performance liquid chromatography. J Clin Microbiol 29:2468–2472
    [Google Scholar]
  8. Butler W. R., Thibert L., Kilburn J. O. 1992; Identification of Mycobacterium avium complex strains and some similar species by high-performance liquid chromatography. J Clin Microbiol 30:2698–2704
    [Google Scholar]
  9. Grange J. M., Stanford J. L. 1974; Reevaluation of Mycobacterium fortuitum (synonym: Mycobacterium ranae ). Int J Syst Bacteriol 24:320–329 [CrossRef]
    [Google Scholar]
  10. Grimont F., Grimont P. A. 1986; Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol 137B:165–175
    [Google Scholar]
  11. Hall J. A. 1999; BioEdit: a user-friendly sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  12. Ingram C. W., Tanner D. C., Durack D. T., Kernodle G. W. Jr, Corey G. R. 1993; Disseminated infection with rapidly growing mycobacteria. Clin Infect Dis 16:463–471 [CrossRef]
    [Google Scholar]
  13. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  14. Kiehlbauch J. A., Plikaytis B. D., Swaminathan B., Cameron D. N., Wachsmuth I. K. 1991; Restriction fragment length polymorphisms in the ribosomal genes for species identification and subtyping of aerotolerant Campylobacter species. J Clin Microbiol 29:1670–1676
    [Google Scholar]
  15. Kirschner P., Kiekenbeck M., Meissner D., Wolters J., Böttger E. C. 1992; Genetic heterogeneity within Mycobacterium fortuitum complex species: genotypic criteria for identification. J Clin Microbiol 30:2772–2775
    [Google Scholar]
  16. Kirschner P., Springer B., Vogel U., Meier A., Wrede A., Keikenbeck M., Bange F. C., Böttger E. C. 1993; Genotypic identification of mycobacteria by nucleic acid sequencing determination: report of a 2 year experience in a clinical laboratory. J Clin Microbiol 31:2882–2889
    [Google Scholar]
  17. Kusunoki S., Ezaki T. 1992; Proposal of Mycobacterium peregrinum sp. nov., nom. rev., and elevation of Mycobacterium chelonae subsp. abscessus (Kubica et al .) to species status: Mycobacterium abscessus comb. nov. Int J Syst Bacteriol 42:240–245 [CrossRef]
    [Google Scholar]
  18. Lasker B. A., Brown J. M., McNeil M. M. 1992; Identification and epidemiological typing of clinical and environmental isolates of the genus Rhodococcus with use of a digoxigenin-labelled rDNA gene probe. Clin Infect Dis 15:223–233 [CrossRef]
    [Google Scholar]
  19. Loeffelholz M. J., Scholl D. R. 1989; Method for improved extraction of DNA from Nocardia asteroides . J Clin Microbiol 27:1880–1881
    [Google Scholar]
  20. Mandel M., Igambi L., Bergendahl J., Dodson M. L. Jr, Scheltgen E. 1970; Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. J Bacteriol 101:333–338
    [Google Scholar]
  21. NCCLS 1997 Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically . Approved standard M7-A4 Wayne, PA: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  22. Popovic T. C., Bopp C. A., Olsvik O., Kiehlbauch J. 1993; Ribotyping in molecular epidemiology. In Diagnostic Molecular Microbiology pp  573–583 Edited by Persing D. H., Smith T. F., Tenover F. C., White T. J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Scheidl T. M., Miura Y., Yee H. A., Tamaoki T. 1995; Automated fluorescent dye-terminator sequencing of G+C-rich tracts with the aid of dimethyl sulfoxide. Biotechniques 19:691–694
    [Google Scholar]
  25. Schinsky M. F., McNeil M. M., Whitney A. M., Steigerwalt A. G., Lasker B. A., Floyd M. M., Hogg G. G., Brenner D. J., Brown J. M. 2000; Mycobacterium septicum sp. nov., a new rapidly growing species associated with catheter-related bacteraemia. Int J Syst Evol Microbiol 50:575–581 [CrossRef]
    [Google Scholar]
  26. Silcox V. A., Good R. C., Floyd M. M. 1981; Identification of clinically significant Mycobacterium fortuitum complex isolates. J Clin Microbiol 14:686–691
    [Google Scholar]
  27. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  28. Steingrube V. A., Gibson J. L., Brown B. A., Zhang Y., Wilson R. W., Rajagopalan M., Wallace R. J. Jr 1995; PCR amplification and restriction endonuclease analysis of a 65-kilodalton heat shock protein gene sequence for taxonomic separation of rapidly growing mycobacteria. J Clin Microbiol 33:149–153
    [Google Scholar]
  29. Steingrube V. A., Wilson R. W., Brown B. A., Jost K. C. Jr, Blacklock Z., Gibson J. L., Wallace R. J. Jr 1997; Rapid identification of clinically significant species and taxa of aerobic actinomycetes, including Actinomadura , Gordona , Nocardia , Rhodococcus , Streptomyces , and Tsukamurella isolates by DNA amplification and restriction endonuclease analysis. J Clin Microbiol 35:817–822
    [Google Scholar]
  30. Takeuchi M., Hatano K. 1998; Gordonia rhizosphera sp. nov. isolated from the mangrove rhizosphere. Int J Syst Bacteriol 48:907–912 [CrossRef]
    [Google Scholar]
  31. Telenti A., Marchesi F., Balz M., Bally F., Böttger E. C., Bodmer T. 1993; Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31:175–178
    [Google Scholar]
  32. Thompson J. D., Gibson T. J., Plewniak F. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  33. Tsukamura M., Nemoto H., Yugi H. 1983; Mycobacterium porcinum sp. nov., a porcine pathogen. Int J Syst Bacteriol 33:162–165 [CrossRef]
    [Google Scholar]
  34. Turenne C. Y., Tschetter L., Wolfe J., Kabani A. 2001; Necessity of quality-controlled 16S rRNA gene sequence databases: identifying nontuberculous Mycobacterium species. J Clin Microbiol 39:3637–3648 [CrossRef]
    [Google Scholar]
  35. Vestal A. L. 1975; Procedures for the Isolation and Identification of Mycobacteria . Publication (CDC) 76-8230 Atlanta, GA: Centers for Disease Control;
    [Google Scholar]
  36. Wallace R. J. Jr, Swenson J. M., Silcox V. A., Good R. C., Tschen J. A., Stone M. S. 1983; Spectrum of disease due to rapidly growing mycobacteria. Rev Infect Dis 5:657–679 [CrossRef]
    [Google Scholar]
  37. Wallace R. J. Jr, Brown B. A., Silcox V. A. 8 other authors 1991; Clinical disease, drug susceptibility, and biochemical patterns of the unnamed third biovariant complex of Mycobacterium fortuitum . J Infect Dis 163:598–603 [CrossRef]
    [Google Scholar]
  38. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  39. Weyant R. S., Moss C. W., Weaver R. E., Hollis D. G., Jordan J. J., Cook E. C., Daneshvar M. I. 1996 Identification of Unusual Pathogenic Gram-negative Aerobic and Facultatively Anaerobic Bacteria , 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  40. Wilson R. W., Steingrube V. A., Brown B. A., Wallace R. J. Jr 1998; Clinical application of PCR-restriction enzyme pattern analysis for rapid identification of aerobic actinomycete isolates. J Clin Microbiol 36:148–152
    [Google Scholar]
  41. Yassin A. F., Brzezinka H., Schaal K. P. 1993; Cellular fatty acid methyl ester profiles as a tool in the differentiation of members of the genus Mycobacterium . Zentralbl Bakteriol 279:316–329 [CrossRef]
    [Google Scholar]
  42. Yassin A. F., Rainey F. A., Brzezinka H., Burghardt J., Lee H. J., Schaal K. P. 1995; Tsukamurella inchonensis sp. nov. Int J Syst Bacteriol 45:522–527 [CrossRef]
    [Google Scholar]
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