sp. nov., a novel actinobacterium isolated from marine sediment Free

Abstract

An actinobacterial strain was isolated from marine sediment taken from the Troitsa Bay of the Gulf of Peter the Great, East Siberian Sea, and subjected to a taxonomic investigation. The isolate, designated KMM 3905, was Gram-positive, aerobic, non-motile and coccoid. The strain tolerated up to 15 % NaCl in growth media, although its presence was not essential for growth. Chemotaxonomic and phylogenetic properties of the strain were consistent with its classification in the genus . The strain formed an independent phylogenetic line in the 16S rRNA gene tree encompassed by members of . Comparison of phenotypic characteristics also supported its assignment to a novel species within . The name sp. nov. (type strain KMM 3905=KCTC 9943) is proposed for this halotolerant actinobacterium from the marine environment.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02742-0
2004-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/5/ijs541617.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02742-0&mimeType=html&fmt=ahah

References

  1. Boháček J., Kocur M., Martinec T. 1969; Deoxyribonucleic acid base composition of Micrococcus roseus . Antonie van Leeuwenhoek 35:185–188 [CrossRef]
    [Google Scholar]
  2. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1993 phylip (Phylogenetic Inference Package), version 3.5c. Distributed by the author Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  4. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284 [CrossRef]
    [Google Scholar]
  5. Han S. K., Nedashkovskaya O. I., Mikhailov V. V., Kim S. B., Bae K. S. 2003; Salinibacterium amurskyense gen. nov., sp. nov. a novel genus of the family Microbacteriaceae from the marine environment. Int J Syst Evol Microbiol 53:2061–2066 [CrossRef]
    [Google Scholar]
  6. Kim S. B., Falconer C., Williams E., Goodfellow M. 1998; Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species isolated from soil. Int J Syst Bacteriol 48:59–68 [CrossRef]
    [Google Scholar]
  7. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  8. Kloos W. E. 1969; Transformation of Micrococcus lysodeikticus by various members of the family Micrococcaceae . J Gen Microbiol 59:247–255 [CrossRef]
    [Google Scholar]
  9. Kloos W. E., Tornabene T. G., Schleifer K. H. 1974; Isolation and characterization of micrococci from human skin, including two new species: Micrococcus lylae and Micrococcus kristinae . Int J Syst Bacteriol 24:79–101 [CrossRef]
    [Google Scholar]
  10. Kocur M. 1986; Genus Micrococcus Cohn 1872. In Bergey's Manual of Systematic Bacteriology vol 2 pp  1004–1008 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  11. Kocur M., Bergan T., Mortensen N. 1971; DNA base composition of Gram-positive cocci. J Gen Microbiol 69:167–183 [CrossRef]
    [Google Scholar]
  12. Kocur M., Kloos W. E., Schleifer K. H. 1991; The genus Micrococcus . In The Prokaryotes , 2nd edn. vol 2 pp  1300–1311 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  13. Kovács G., Burghardt J., Pradella S., Schumann P., Stackebrandt E., Màrialigeti K. 1999; Kocuria palustris sp. nov. and Kocuria rhizophila sp. nov., isolated from the rhizoplane of the narrow-leaved cattail ( Typha angustifolia . Int J Syst Bacteriol 49:167–173 [CrossRef]
    [Google Scholar]
  14. Minnikin D. E., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  15. Ogasawara-Fujita N., Sakaguchi K. 1976; Classification of micrococci on the basis of deoxyribonucleic acid homology. J Gen Microbiol 94:97–106 [CrossRef]
    [Google Scholar]
  16. Reddy G. S. N., Prakash J. S. S., Prabahar V., Matsumoto G. I., Stackebrandt E., Shivaji S. 2003; Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 53:183–187 [CrossRef]
    [Google Scholar]
  17. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  18. Stackebrandt E., Koch C., Gvozdiak O., Schumann P. 1995; Taxonomic dissection of the genus Micrococcus : Kocuria gen.nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45:682–692 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02742-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02742-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed