A Gram-staining-negative bacterium, designated strain BLN2T, was isolated from within the roots of reeds (Phragmites australis) in Beijing Cuihu Wetland (China) and characterized using a polyphasic taxonomic approach. The cells were yellow-pigmented, rod-shaped, strictly aerobic and devoid of flagella, but showed gliding motility. Strain BLN2T produced yellow, translucent, circular and convex colonies, with optimal growth at 30 °C and pH 7.0. The major respiratory quinone was menaquinone 6 (MK-6) and the predominant fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 3-OH, C16 : 0, iso-C17 : 0 3-OH and iso-C15 : 0 3-OH. The G+C content of the genomic DNA was 34.8 mol%. The 16S rRNA gene sequence analysis showed that strain BLN2T belonged to the genus Flavobacterium and was most closely related to Flavobacterium anhuiense CGMCC 1.6859T (97.0 % sequence similarity). The DNA–DNA relatedness between strain BLN2T and F. anhuiense CGMCC 1.6859T was 25.7 %. Based on the phenotypic data and phylogenetic inference presented, it is concluded that strain BLN2T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium phragmitis sp. nov. is proposed. The type strain is BLN2T ( = DSM 23314T = CGMCC 1.10370T).
Published Online:
Funding
This study was supported by the:
Scientific Research Program of Beijing Municipal Commission of Education
BernardetJ. F.,
SegersP.,
VancanneytM.,
BertheF.,
KerstersK.,
VandammeP.1996; Cutting a Gordian knot: Emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [View Article]
BernardetJ. F.,
NakagawaY.,
HolmesB.Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
ChunJ.,
LeeJ. H.,
JungY.,
KimM.,
KimS.,
KimB. K.,
LimY. W.2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
DongX.-Z.,
CaiM.-Y.
(editors) 2001; Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria pp. 370–398 Beijing: Science Press; (in Chinese)
GillisM.,
De LeyJ.,
De CleeneM.1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur J Biochem 12:143–153 [View Article][PubMed]
HornM. A.,
IhssenJ.,
MatthiesC.,
SchrammA.,
AckerG.,
DrakeH. L.2005; Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa
. Int J Syst Evol Microbiol 55:1255–1265 [View Article][PubMed]
LaneD. J.1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175 Edited by
StackebrandtE.,
GoodfellowM.
Chichester: Wiley;
LiuY.,
JinJ. H.,
ZhouY. G.,
LiuH. C.,
LiuZ. P.2010; Flavobacterium caeni sp. nov., isolated from a sequencing batch reactor for the treatment of malachite green effluents. Int J Syst Evol Microbiol 60:417–421 [View Article][PubMed]
MarmurJ.,
DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
SmibertR. M.,
KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
StackebrandtE.,
FrederiksenW.,
GarrityG. M.,
GrimontP. A. D.,
KämpferP.,
MaidenM. C. J.,
NesmeX.,
Rosselló-MoraR.,
SwingsJ. et al.2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [View Article][PubMed]