1887

Abstract

A mesophilic, aerotolerant, endospore-forming, fermentative bacterium, designated strain B1, was isolated from soil polluted by crude oil in the Karamay Oil Field, China. Cells were Gram-positive, rod-shaped, 1.1–1.6 µm wide and 2.3–4.7 µm long, and were motile by means of peritrichous flagella. Growth occurred at 10–40 °C and pH 6.0–8.9; optimal growth occurred at 28–32 °C and pH 7.3. The optimal concentrations of NaCl and sea salts for growth were 0.5 and 1 % (w/v), respectively. The strain was halotolerant and grew in the presence of NaCl or sea salts up to a concentration of 9 % (w/v). Substrates utilized as sole carbon sources were beef extract, yeast extract, peptone, tryptone, casein, -glucose, -fructose, -xylose, -ribose, -galactose, maltose, -rhamnose, trehalose, -valine, -alanine plus -proline and -alanine plus -glycine. The main products of glucose fermentation were ethanol and acetate. iso-C, iso-C, C and iso-C were the major fatty acids. 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus , showing 94.7 and 94.1 % similarity to the type strains of and , respectively. The genomic DNA G+C content of strain B1 was 29.8 mol%. On the basis of its phenotypic and genotypic properties, strain B1 is suggested to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is B1 ( = CGMCC 1.5148  = JCM 16806).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30970002)
  • National Science and Technology Major Projects - water pollution control and treatment project (Award 2008ZX07101-006-3)
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2012-01-01
2024-03-29
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References

  1. Alain K., Pignet P., Zbinden M., Quillevere M., Duchiron F., Donval J. P., Lesongeur F., Raguenes G., Crassous P. et al. other authors 2002; Caminicella sporogenes gen. nov., sp. nov., a novel thermophilic spore-forming bacterium isolated from an East-Pacific Rise hydrothermal vent. Int J Syst Evol Microbiol 52:1621–1628 [View Article][PubMed]
    [Google Scholar]
  2. Bryant M. P. 1972; Commentary on the Hungate technique for culture of anaerobic bacteria. Am J Clin Nutr 25:1324–1328[PubMed]
    [Google Scholar]
  3. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  4. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  5. Grishchenkov V. G., Townsend R. T., McDonald T. J., Autenrieth R. L., Bonner J. S., Boronin A. M. 2000; Degradation of petroleum hydrocarbons by facultative anaerobic bacteria under aerobic and anaerobic conditions. Process Biochem 35:889–896 [View Article]
    [Google Scholar]
  6. Hungate R. E. 1969; A roll tube method for cultivation of strict anaerobes. In Methods in Microbiology vol. 3B pp. 117–132 Edited by Norris J. R., Ribbons R. W. London: Academic Press; [View Article]
    [Google Scholar]
  7. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  8. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [View Article]
    [Google Scholar]
  9. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  11. Ogg C. D., Patel B. K. C. 2009; Thermotalea metallivorans gen. nov., sp. nov., a thermophilic, anaerobic bacterium from the Great Artesian Basin of Australia aquifer. Int J Syst Evol Microbiol 59:964–971 [View Article][PubMed]
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  13. Slobodkin A. I., Tourova T. P., Kostrikina N. A., Chernyh N. A., Bonch-Osmolovskaya E. A., Jeanthon C., Jones B. E. 2003; Tepidibacter thalassicus gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, fermentative bacterium from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 53:1131–1134 [View Article][PubMed]
    [Google Scholar]
  14. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  15. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  16. Urios L., Cueff V., Pignet P., Barbier G. 2004; Tepidibacter formicigenes sp. nov., a novel spore-forming bacterium isolated from a Mid-Atlantic Ridge hydrothermal vent. Int J Syst Evol Microbiol 54:439–443 [View Article][PubMed]
    [Google Scholar]
  17. Widdel F., Kohring G. W., Mayer F. 1983; Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. Arch Microbiol 134:286–294 [View Article]
    [Google Scholar]
  18. Zhang X. Q., Ying Y., Ye Y., Xu X. W., Zhu X. F., Wu M. 2010; Thermus arciformis sp. nov., a thermophilic species from a geothermal area. Int J Syst Evol Microbiol 60:834–839 [View Article][PubMed]
    [Google Scholar]
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