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Abstract

A mesophilic, aerotolerant, endospore-forming, fermentative bacterium, designated strain B1, was isolated from soil polluted by crude oil in the Karamay Oil Field, China. Cells were Gram-positive, rod-shaped, 1.1–1.6 µm wide and 2.3–4.7 µm long, and were motile by means of peritrichous flagella. Growth occurred at 10–40 °C and pH 6.0–8.9; optimal growth occurred at 28–32 °C and pH 7.3. The optimal concentrations of NaCl and sea salts for growth were 0.5 and 1 % (w/v), respectively. The strain was halotolerant and grew in the presence of NaCl or sea salts up to a concentration of 9 % (w/v). Substrates utilized as sole carbon sources were beef extract, yeast extract, peptone, tryptone, casein, -glucose, -fructose, -xylose, -ribose, -galactose, maltose, -rhamnose, trehalose, -valine, -alanine plus -proline and -alanine plus -glycine. The main products of glucose fermentation were ethanol and acetate. iso-C, iso-C, C and iso-C were the major fatty acids. 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus , showing 94.7 and 94.1 % similarity to the type strains of and , respectively. The genomic DNA G+C content of strain B1 was 29.8 mol%. On the basis of its phenotypic and genotypic properties, strain B1 is suggested to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is B1 ( = CGMCC 1.5148  = JCM 16806).

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2012-01-01
2019-10-20
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