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Abstract
A mesophilic, aerotolerant, endospore-forming, fermentative bacterium, designated strain B1T, was isolated from soil polluted by crude oil in the Karamay Oil Field, China. Cells were Gram-positive, rod-shaped, 1.1–1.6 µm wide and 2.3–4.7 µm long, and were motile by means of peritrichous flagella. Growth occurred at 10–40 °C and pH 6.0–8.9; optimal growth occurred at 28–32 °C and pH 7.3. The optimal concentrations of NaCl and sea salts for growth were 0.5 and 1 % (w/v), respectively. The strain was halotolerant and grew in the presence of NaCl or sea salts up to a concentration of 9 % (w/v). Substrates utilized as sole carbon sources were beef extract, yeast extract, peptone, tryptone, casein, d-glucose, d-fructose, d-xylose, d-ribose, d-galactose, maltose, l-rhamnose, trehalose, l-valine, dl-alanine plus l-proline and dl-alanine plus l-glycine. The main products of glucose fermentation were ethanol and acetate. iso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C13 : 0 were the major fatty acids. 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus Tepidibacter, showing 94.7 and 94.1 % similarity to the type strains of Tepidibacter formicigenes and Tepidibacter thalassicus, respectively. The genomic DNA G+C content of strain B1T was 29.8 mol%. On the basis of its phenotypic and genotypic properties, strain B1T is suggested to represent a novel species of the genus Tepidibacter, for which the name Tepidibacter mesophilus sp. nov. is proposed. The type strain is B1T ( = CGMCC 1.5148T = JCM 16806T).
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Funding
- National Natural Science Foundation of China (Award 30970002)
- National Science and Technology Major Projects - water pollution control and treatment project (Award 2008ZX07101-006-3)