1887

Abstract

A Gram-negative bacterium designated LA1 was isolated from water collected in hypersaline Lake Laysan on Laysan Island in the Northwestern Hawaiian Islands. Cells occurred singly as fine rods to short filaments. Growth in 50 % strength marine broth occurred optimally when the medium contained 7·5–10 % (w/v) NaCl. The major fatty acids in LA1 grown at 15 and 30 °C were 12-methyl tetradecanoic acid and 13-methyl tetradecanoic acid, respectively. The nucleotide sequence of the 16S rRNA gene showed that LA1 belonged in the (CFB) group in the domain . The closest described neighbour in terms of 16S rRNA gene sequence identity was ACAM 623 (94·4 % over 1423 bases), an obligate psychrophile from Antarctic sea-ice. The G+C content of 35·0 mol% was consistent with this affiliation. Phenotypic and genotypic analyses, including DNA hybridization, indicated that LA1 could be assigned to the genus but, based on significant differences, including growth at 43 °C, it constitutes a novel species, sp. nov., for which LA1 (=ATCC BAA-734=DSM 15496) is the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02733-0
2004-05-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/3/ijs540935.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02733-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M. 1997 In Handbook of Microbiological Media , p. 7 Edited by Parks L. C. Boca Raton, FL: CRC Press;
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  3. Bowman J. P., McCammon S. A., Lewis T., Skerratt J. H., Brown J. L., Nichols D. S., McMeekin T. A. 1998; Psychroflexus torquis gen. nov., sp. nov., a psychrophilic species from Antarctic sea ice, and reclassification of Flavobacterium gondwanense (Dobson et al ., 1993) as Psychroflexus gondwanense gen. nov., comb. nov. Microbiology 144:1601–1609 [CrossRef]
    [Google Scholar]
  4. Dobson S. J., Colwell R. R., McMeekin T. A., Franzmann P. D. 1993; Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake. Int J Syst Bacteriol 43:77–83 [CrossRef]
    [Google Scholar]
  5. Donachie S. P., Christenson B. W., Kunkel D. D., Malahoff A., Alam M. 2002; Microbial community in acidic hydrothermal waters of volcanically active White Island. New Zealand. Extremophiles 6:419–425 [CrossRef]
    [Google Scholar]
  6. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  7. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp  115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  8. Manz W., Wendt-Potthoff K., Neu T. R., Szewzyk U., Lawrence J. R. 1999; Phylogenetic composition, spatial structure, and dynamics of lotic bacterial biofilms investigated by fluorescence in situ hybridization and confocal laser scanning microscopy. Microb Ecol 37:225–237 [CrossRef]
    [Google Scholar]
  9. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  10. Page R. D. M. 1996; TREEVIEW: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
    [Google Scholar]
  11. Perrière G., Gouy M. 1996; WWW-Query: an on-line retrieval system for biological sequence banks. Biochimie 78:364–369 [CrossRef]
    [Google Scholar]
  12. Ravenschlag K., Sahm K., Amann R. 2001; Quantitative molecular analysis of the microbial community in marine Arctic sediments (Svalbard. Appl Environ Microbiol 67:387–395 [CrossRef]
    [Google Scholar]
  13. Reichenbach H. 1989; Order I. Cytophagales Leadbetter 1974. In Bergey's Manual of Systematic Bacteriology vol 3 pp  2011–2013 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  15. Sasser M. 1997; Identification of bacteria by gas chromatography of cellular fatty acids . MIDI Technical Note 101: Newark, DE: MIDI Inc;
    [Google Scholar]
  16. Sly L. I., Blackall L. L., Kraat P. C., Tian-Shen T., Sangkhobol V. 1986; The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5:139–156 [CrossRef]
    [Google Scholar]
  17. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  18. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02733-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02733-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error