1887

Abstract

A Gram-negative, motile, psychrotolerant, oxidase- and catalase-positive bacterium, designated BSs20135, was isolated from Arctic marine sediment. Cells were straight or slightly curved rods and formed circular, convex and yellowish-brown colonies. Buds and prosthecae could be produced. The strain grew at 4–28 °C (optimum 25 °C) and with 1–5 % (w/v) NaCl (optimum 2 %) and hydrolysed aesculin and DNA, but did not reduce nitrate to nitrite. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain BSs20135 belonged to the genus and shared 93.6–97.7 % sequence similarity with the type strains of known species of the genus . The major cellular fatty acids of strain BSs20135 were summed feature 3 (comprising Cω7 and/or iso-C 2-OH), C, Cω8 and Cω7. The genomic DNA G+C content was 40.3 mol%. Based on 16S rRNA gene sequence analysis, DNA–DNA hybridization data and phenotypic and chemotaxonomic characterization, strain BSs20135 represents a novel species, for which the name sp. nov. is proposed. The type strain is BSs20135 ( = CCTCC AB 209161  = KACC 14537).

Funding
This study was supported by the:
  • Hi-Tech Research and Development Program of China (Award 2007AA021306 and 2007AA091903)
  • National Natural Science Foundation of China (Award 30500001 and 40706001)
  • COMRA Program (Award DYXM-115-02-2-6)
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2011-10-01
2024-03-29
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References

  1. Baik K. S., Park Y.-D., Seong C. N., Kim E. M., Bae K. S., Chun J. 2006; Glaciecola nitratireducens sp. nov., isolated from seawater. Int J Syst Evol Microbiol 56:2185–2188 [View Article][PubMed]
    [Google Scholar]
  2. Bowman J. P., McCammon S. A., Brown J. L., McMeekin T. A. 1998; Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov.: psychrophilic bacteria from Antarctic sea-ice habitats. Int J Syst Bacteriol 48:1213–1222 [View Article]
    [Google Scholar]
  3. Buck J. D. 1982; Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993[PubMed]
    [Google Scholar]
  4. Chen L.-P., Xu H.-Y., Fu S.-Z., Fan H.-X., Liu Y.-H., Liu S.-J., Liu Z.-P. 2009; Glaciecola lipolytica sp. nov., isolated from seawater near Tianjin city, China. Int J Syst Evol Microbiol 59:73–76 [View Article][PubMed]
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  7. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp. 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  9. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  10. Matsuyama H., Hirabayashi T., Kasahara H., Minami H., Hoshino T., Yumoto I. 2006; Glaciecola chathamensis sp. nov., a novel marine polysaccharide-producing bacterium. Int J Syst Evol Microbiol 56:2883–2886 [View Article][PubMed]
    [Google Scholar]
  11. Romanenko L. A., Zhukova N. V., Rohde M., Lysenko A. M., Mikhailov V. V., Stackebrandt E. 2003; Glaciecola mesophila sp. nov., a novel marine agar-digesting bacterium. Int J Syst Evol Microbiol 53:647–651 [View Article][PubMed]
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  13. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  14. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  15. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  16. Van Trappen S., Tan T.-L., Yang J., Mergaert J., Swings J. 2004; Glaciecola polaris sp. nov., a novel budding and prosthecate bacterium from the Arctic Ocean, and emended description of the genus Glaciecola . Int J Syst Evol Microbiol 54:1765–1771 [View Article][PubMed]
    [Google Scholar]
  17. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  18. Yong J.-J., Park S.-J., Kim H.-J., Rhee S.-K. 2007; Glaciecola agarilytica sp. nov., an agar-digesting marine bacterium from the East Sea, Korea. Int J Syst Evol Microbiol 57:951–953 [View Article][PubMed]
    [Google Scholar]
  19. Zhang D.-C., Yu Y., Chen B., Wang H.-X., Liu H.-C., Dong X.-Z., Zhou P.-J. 2006; Glaciecola psychrophila sp. nov., a novel psychrophilic bacterium isolated from the Arctic. Int J Syst Evol Microbiol 56:2867–2869 [View Article][PubMed]
    [Google Scholar]
  20. Zhang X.-Y., Zhang Y.-J., Yu Y., Li H.-J., Gao Z.-M., Chen X.-L., Chen B., Zhang Y.-Z. 2010; Neptunomonas antarctica sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 60:1958–1961 [View Article][PubMed]
    [Google Scholar]
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