1887

Abstract

A novel Gram-negative, aerobic and moderately thermophilic bacterium, strain 4BON, was isolated from a non-water-flooded Australian terrestrial oil reservoir. Cells were non-spore-forming straight rods, which were motile by means of a polar flagellum. The optimum growth conditions were 55 °C, pH 6·9 and 0·5 % NaCl. Strain 4BON was oxidase- and catalase-positive; it grew on fumarate, pyruvate, succinate, formate, ethanol and yeast extract in the presence of oxygen or nitrate as terminal electron acceptor. Nitrate was reduced to nitrous oxide. The DNA G+C content of the strain was 58·6 mol%. The closest phylogenetic relative of strain 4BON was (similarity of 91·8 %), of the -. As strain 4BON is physiologically and phylogenetically different from , it is proposed that it be assigned to a novel species of a novel genus, gen. nov., sp. nov. The type strain is 4BON (=DSM 15512=CIP 107790).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02732-0
2004-05-01
2020-07-02
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/3/ijs540645.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02732-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum R. J., Woese C. R., Wolfe R. S. 1979; Methanogens: reevaluation of a unique biological group. Microbiol Rev 43:260–296
    [Google Scholar]
  3. Benson D. A., Boguski M. S., Lipman D. J., Ostell J., Ouellette B. F. F., Rapp B. A., Wheeler D. L. 1999; GenBank. Nucleic Acids Res 27:12–17 [CrossRef]
    [Google Scholar]
  4. Cord-Ruwisch R. 1985; A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. J Microbiol Methods 4:33–36 [CrossRef]
    [Google Scholar]
  5. Davydova-Charakhch'yan I. A., Mileeva A. N., Mityushina L. L., Belyaev S. S. 1992; Acetogenic bacteria from oil fields of Tataria and western Siberia. Microbiology (English translation of Mikrobiologiya) 61:208–216
    [Google Scholar]
  6. Fardeau M.-L., Cayol J.-L., Magot M., Ollivier B. 1993; H2 oxidation in the presence of thiosulfate by a Thermoanaerobacter strain isolated from an oil-producing well. FEMS Microbiol Lett 113:327–332 [CrossRef]
    [Google Scholar]
  7. Fardeau M.-L., Ollivier B., Patel B. K. C., Magot M., Thomas P., Rimbault A., Rocchiccioli F., Garcia J.-L. 1997; Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. Int J Syst Bacteriol 47:1013–1019 [CrossRef]
    [Google Scholar]
  8. Fardeau M.-L., Magot M., Patel B. K. C., Thomas P., Garcia J.-L., Ollivier B. 2000; Thermoanaerobacter subterraneus sp. nov., a novel thermophile isolated from oilfield water. Int J Syst Evol Microbiol 50:2141–2149 [CrossRef]
    [Google Scholar]
  9. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  10. Gevertz D., Telang A. J., Voordouw G., Jenneman G. E. 2000; Isolation and characterization of strains CVO and FWKO B, two novel nitrate-reducing, sulfide-oxidizing bacteria isolated from oil field brine. Appl Environ Microbiol 66:2491–2501 [CrossRef]
    [Google Scholar]
  11. Grassia G. S., McLean K. M., Glénat P., Bauld J., Sheehy A. J. 1996; A systematic survey for thermophilic fermentative bacteria and archaea in high temperature petroleum reservoirs. FEMS Microbiol Ecol 21:47–58 [CrossRef]
    [Google Scholar]
  12. Greene A. C., Patel B. K. C., Sheehy A. J. 1997; Deferribacter thermophilus gen. nov., sp. nov. a novel thermophilic manganese- and iron-reducing bacterium isolated from a petroleum reservoir. Int J Syst Bacteriol 47:505–509 [CrossRef]
    [Google Scholar]
  13. Hall T. A. 1999; bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  14. Hayashi N. R., Ishida T., Yokota A., Kodama T., Igarashi Y. 1999; Hydrogenophilus thermoluteolus gen. nov., sp. nov., a thermophilic, facultatively chemolithoautotrophic, hydrogen-oxidizing bacterium. Int J Syst Bacteriol 49:783–786 [CrossRef]
    [Google Scholar]
  15. Hungate R. E. 1969; A roll-tube method for the cultivation of strict anaerobes. Methods Microbiol 3B:117–132
    [Google Scholar]
  16. Huu N. B., Denner E. B. M., Ha D. T. C., Wanner G., Stan-Lotter H. 1999; Marinobacter aquaeolei sp. nov., a halophilic bacterium isolated from a Vietnamese oil-producing well. Int J Syst Bacteriol 49:367–375 [CrossRef]
    [Google Scholar]
  17. Ivanov M. V., Belayaev S. S., Zyakun A. M., Bondars V., Laurinivicius K. 1983; Microbiological methane formation in oil field development. Geokhimiya 11:1647–1654
    [Google Scholar]
  18. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  19. Knapp J. S., Clark V. L. 1984; Anaerobic growth of Neisseria gonorrhoeae coupled to nitrite reduction. Infect Immun 46:176–181
    [Google Scholar]
  20. Koussémon M., Combet-Blanc Y., Patel B. K. C., Cayol J.-L., Thomas P., Garcia J.-L., Ollivier B. 2001; Propionibacterium microaerophilum sp. nov., a microaerophilic bacterium isolated from olive mill wastewater. Int J Syst Evol Microbiol 51:1373–1382
    [Google Scholar]
  21. LaPara T. M., Nakatsu C. H., Pantea L., Alleman J. E. 2000; Phylogenetic analysis of bacterial communities in mesophilic and thermophilic bioreactors treating pharmaceutical wastewater. Appl Environ Microbiol 66:3951–3959 [CrossRef]
    [Google Scholar]
  22. Magot M., Ollivier B., Patel B. K. C. 2000; Microbiology of petroleum reservoirs. Antonie van Leeuwenhoek 77:103–116 [CrossRef]
    [Google Scholar]
  23. Maidak B. L., Cole J. R., Lilburn T. G. 7 other authors 2001; The RDP-II (Ribosomal Database Project). Nucleic Acids Res 29:173–174 [CrossRef]
    [Google Scholar]
  24. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  25. Miranda-Tello E., Fardeau M. L., Fernandez L., Ramirez F., Cayol J.-L., Thomas P., Garcia J.-L., Ollivier B. 2003; Desulfovibrio capillatus sp. nov., a novel sulfate-reducing bacterium isolated from an oil field separator located in the Gulf of Mexico. Anaerobe 9:97–103 [CrossRef]
    [Google Scholar]
  26. Mueller R. F., Nielsen P. H. 1996; Characterization of thermophilic consortia from two souring oil reservoirs. Appl Environ Microbiol 62:3083–3087
    [Google Scholar]
  27. Myhr S., Torsvik T. 2000; Denitrovibrio acetiphilus , a novel genus and species of dissimilatory nitrate-reducing bacterium isolated from an oil reservoir model column. Int J Syst Evol Microbiol 50:1611–1619 [CrossRef]
    [Google Scholar]
  28. Nilsen R. K., Beeder J., Thorstenson T., Torsvik T. 1996; Distribution of thermophilic marine sulfate reducers in North Sea oil field waters and oil reservoirs. Appl Environ Microbiol 62:1793–1798
    [Google Scholar]
  29. Ollivier B., Fardeau M.-L., Cayol J.-L., Magot M., Patel B. K. C., Prensier G., Garcia J.-L. 1998; Methanocalculus halotolerans gen. nov., sp. nov., isolated from an oil-producing well. Int J Syst Bacteriol 48:821–828 [CrossRef]
    [Google Scholar]
  30. Rees G. N., Patel B. K. C., Grassia G. S., Sheehy A. J. 1997; Anaerobaculum thermoterrenum gen. nov., sp. nov. a novel, thermophilic bacterium which ferments citrate. Int J Syst Bacteriol 47:150–154 [CrossRef]
    [Google Scholar]
  31. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  32. Telang A. J., Ebert S., Foght J. M., Westlake D. W. S., Jenneman G. E., Gevertz D., Voordouw G. 1997; Effect of nitrate injection on the microbial community in an oil field as monitored by reverse sample genome probing. Appl Environ Microbiol 63:1785–1793
    [Google Scholar]
  33. Voordouw G., Armstrong S. M., Reimer M. F., Fouts B., Telang A. J., Shen Y., Gevertz D. 1996; Characterization of 16S rRNA genes from oil field microbial communities indicates the presence of a variety of sulfate-reducing, fermentative, and sulfide-oxidizing bacteria. Appl Environ Microbiol 62:1623–1629
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02732-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02732-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error