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Abstract

A Gram-negative, motile, non-spore-forming, rod-shaped strain, TF-27 (=KCCM 41648=JCM 11814), was isolated from a tidal flat in Korea. This organism grew well at 25–35 °C, with optimum growth at 30 °C. Strain TF-27 grew optimally in the presence of 2 % NaCl; it did not grow without NaCl or in the presence of >8 % NaCl. Strain TF-27 simultaneously contained both menaquinones and ubiquinones as isoprenoid quinones. The predominant menaquinone was MK-7 and the predominant ubiquinones were Q-7 and Q-8. The major fatty acids in strain TF-27 were iso-C (20·6 %) and iso-C 2-OH and/or C 7 (21·1 %). The DNA G+C content of strain TF-27 was 42 mol%. Phylogenetic analyses based on 16S rDNA sequences showed that strain TF-27 falls within the radiation of the cluster that is encompassed by the genus . Levels of 16S rDNA sequence similarity between strain TF-27 and the type strains of species were 93·2–96·8 %. On the basis of phenotypic properties and phylogenetic data, strain TF-27 should be placed in the genus as a novel species, for which the name sp. nov. is proposed.

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2004-03-01
2020-01-26
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References

  1. Aguirre, A. A., Balazs, G. H., Zimmerman, B. & Spraker, T. R. ( 1994; ). Evaluation of Hawaiian green turtles (Cheledonia mydas) for potential pathogens associated with fibropapillomas. J Wildl Dis 30, 8–15.[CrossRef]
    [Google Scholar]
  2. Anzai, Y., Kim, H., Park, J.-Y., Wakabayashi, H. & Oyaizu, H. ( 2000; ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef]
    [Google Scholar]
  3. Baumann, P. & Baumann, L. ( 1981; ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In The Prokaryotes. A Handbook on Habitats, Isolation, and Identification of Bacteria, pp. 1302–1331. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. Berlin: Springer.
  4. Boone, D. R., Castenholz, R. W. & Garrity, G. M. ( 2001; ). Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1. New York: Springer.
  5. Bowman, J. P., McCammon, S. A., Nichols, D. S., Skerratt, J. H., Rea, S. M., Nichols, P. D. & McMeekin, T. A. ( 1997; ). Shewanella gelidimarina sp. nov. and Shewanella frigidimarina sp. nov., novel Antarctic species with the ability to produce eicosapentaenoic acid (20 : 5ω3) and grow anaerobically by dissimilatory Fe(III) reduction. Int J Syst Bacteriol 47, 1040–1047.[CrossRef]
    [Google Scholar]
  6. Bozal, N., Montes, M. J., Tudela, E., Jiménez, F. & Guinea, J. ( 2002; ). Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. Int J Syst Evol Microbiol 52, 195–205.
    [Google Scholar]
  7. Brink, A. J., van Straten, A. & van Rensburg, A. J. ( 1995; ). Shewanella (Pseudomonas) putrefaciens bacteremia. Clin Infect Dis 20, 1327–1332.[CrossRef]
    [Google Scholar]
  8. Bruns, A., Rohde, M. & Berthe-Corti, L. ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef]
    [Google Scholar]
  9. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  10. Ivanova, E. P., Sawabe, T., Gorshkova, N. M., Svetashev, V. I., Mikhailov, V. V., Nicolau, D. V. & Christen, R. ( 2001; ). Shewanella japonica sp. nov. Int J Syst Evol Microbiol 51, 1027–1033.[CrossRef]
    [Google Scholar]
  11. Jorgensen, B. R. & Huß, H. H. ( 1989; ). Growth and activity of Shewanella putrefaciens isolated from spoiling fish. Int J Food Microbiol 9, 51–62.[CrossRef]
    [Google Scholar]
  12. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19, 161–206.
    [Google Scholar]
  13. Kostka, J. E., Stucki, J. W., Nealson, K. H. & Wu, J. ( 1996; ). Reduction of structural Fe(III) in smectite by a pure culture of Shewanella putrefaciens strain MR-1. Clays Clay Miner 44, 522–529.[CrossRef]
    [Google Scholar]
  14. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  15. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  16. Levring, T. ( 1946; ). Some culture experiments with Ulva and artificial seawater. Kungl Fysiogr Sallsk Lund Forh 16, 45–56.
    [Google Scholar]
  17. Lovley, D. R. & Phillips, E. J. P. ( 1988; ). Novel mode of microbial energy metabolism: organic carbon oxidation coupled to dissimilatory reduction of iron or manganese. Appl Environ Microbiol 54, 1472–1480.
    [Google Scholar]
  18. MacDonell, M. T. & Colwell, R. R. ( 1985; ). Phylogeny of the Vibrionaceae, and recommendation for two new genera, Listonella and Shewanella. Syst Appl Microbiol 6, 171–182.[CrossRef]
    [Google Scholar]
  19. Myers, C. R. & Nealson, K. H. ( 1988; ). Bacterial manganese reduction and growth with manganese oxide as the sole electron acceptor. Science 240, 1319–1321.[CrossRef]
    [Google Scholar]
  20. Nealson, K. H., Myers, C. R. & Wimpee, B. ( 1991; ). Isolation and identification of manganese reducing bacteria, and estimates of microbial manganese reducing potential in the Black Sea. Deep Sea Res 38, S907–S920.[CrossRef]
    [Google Scholar]
  21. Nogi, Y., Kato, C. & Horikoshi, K. ( 1998; ). Taxonomic studies of deep-sea barophilic Shewanella strains and description of Shewanella violacea sp. nov. Arch Microbiol 170, 331–338.[CrossRef]
    [Google Scholar]
  22. Nozue, H., Hayashi, T., Hashimoto, Y., Ezaki, T., Hamasaki, K., Ohwada, K. & Terawaki, Y. ( 1992; ). Isolation and characterization of Shewanella alga from human clinical specimens and emendation of the description of S. alga Simidu et al., 1990, 335. Int J Syst Bacteriol 42, 628–634.[CrossRef]
    [Google Scholar]
  23. Perry, K. A., Kostka, J. E., Luther, G. W., III & Nealson, K. H. ( 1993; ). Mediation of sulfur speciation by a Black Sea facultative anaerobe. Science 259, 801–803.[CrossRef]
    [Google Scholar]
  24. Petrovskis, E. A., Vogel, T. M. & Adriaens, P. ( 1994; ). Effects of electron acceptors and donors on transformation of tetrachloromethane by Shewanella putrefaciens MR-1. FEMS Microbiol Lett 121, 357–364.[CrossRef]
    [Google Scholar]
  25. Semple, K. M. & Westlake, D. W. S. ( 1987; ). Characterization of iron-reducing Alteromonas putrefaciens strains from oil field fluids. Can J Microbiol 33, 366–371.[CrossRef]
    [Google Scholar]
  26. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  27. Stackebrandt, E., Murray, R. G. E. & Trüper, H. G. ( 1988; ). Proteobacteria classis nov., a name for the phylogenetic taxon that includes the “purple bacteria and their relatives”. Int J Syst Bacteriol 38, 321–325.[CrossRef]
    [Google Scholar]
  28. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  29. Venkateswaran, K., Moser, D. P., Dollhopf, M. E. & 10 other authors ( 1999; ). Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol 49, 705–724.[CrossRef]
    [Google Scholar]
  30. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef]
    [Google Scholar]
  31. Yoon, J.-H., Lee, S. T. & Park, Y.-H. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef]
    [Google Scholar]
  32. Yoon, J.-H., Kim, I.-G., Shin, D.-Y., Kang, K. H. & Park, Y.-H. ( 2003; ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53, 53–57.[CrossRef]
    [Google Scholar]
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vol. , part 2, pp. 487 – 491

Neighbour-joining tree showing the phylogenetic position of strain TF-27 and representatives of other related taxa [PDF](170 KB)



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