1887

Abstract

A Gram-negative, aerobic, moderately halophilic bacterium, designated Set74, was isolated from brine of a salt concentrator at Ain Oulmene, Algeria. The strain grew optimally at 37–40 °C, at pH 6.5–7.0 and with 5–7.5 % (w/v) NaCl and used various organic compounds as sole carbon, nitrogen and energy sources. Ubiquinone 9 (Q-9) was the major lipoquinone. The main cellular fatty acids were C, Cω9, summed feature 7 (ECL 18.846; C cyclo ω10 and/or Cω6), C 3-OH, Cω9, C and C. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The G+C content of the genomic DNA was 57.4 mol%. The 16S rRNA gene sequence analysis indicated that strain Set74 was a member of the genus . The closest relatives of strain Set74 were NKSG1 (97.5 % 16S rRNA gene sequence similarity) and DD-M3 (97.4 %). DNA–DNA relatedness between strain Set74 and DSM 21262 and DSM 17924 was 45 and 37 %, respectively. On the basis of the phenotypic, chemotaxonomic and phylogenetic features, a novel species, sp. nov., is proposed. The type strain is Set74 ( = CECT 7499  = DSM 22359).

Funding
This study was supported by the:
  • Agencia Española de Cooperación Internacional, Spain (Award A/9572/07)
  • Junta de Andalucía, Spain (Award BIO-190)
  • Algerian Ministry of Higher Education and Scientific Research
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2011-09-01
2021-10-21
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